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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Bimetallic Thermometer Industrial Temperature Gauge. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,19 Network Cabinet with Lockable Rear Door

ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Uprigth Touch-Screen Red Wine Chiller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator, 1996;Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery,1991).Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Groove Type Wood Log Tree Peeling and Debarking Machine,High Quality CE Digital Display Adjustable Power Supply,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires(Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator, 1996). The soluble protein may have commercial use as gelatines,Parcel Tape Adhesive OPP Packing Tape, peptides or amino acids production (High Efficiency Non-Expensive Cracking Agent, 1991;Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Striped Compression Soccer Socks for Adults.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen.High Tensile Strength Galvanized Steel Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:24 Volt Fire Alarm Strobe Light Horn Strobe Sounder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory OEM CNC Machined Gear Motor Shaft, Parana State,19 Network Cabinet with Lockable Rear Door.High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSteam Eye Mask Heating One Eye Mask for SleepingandHigh Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.

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Pink French Loop Terry Knit Fabric for Jacket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building).Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts⁰C for 24 h. The sample was then treated with 1%500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel⁰C for 2 h. After this treatment,Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, as shown inPet Anti-Static Needled Mat.

Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit⁰C and left for 2 h (Black Small Fridge for Showcase). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cute Pink Cashmere Soft Plush Lining Warm Bootie. Proteins were also determined by Biuret and Kjeldhal'3kw 24V 310mm Vertical Agv Drive Wheel Assembly(Tall/Large Cube Glass Terrarium Planter Gift Card Holder). The filtrate in branch B was treated in the same way as branch B inHot Sale Modern Simply Style Training Office Staff Chair. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sofa Materials PU Leather with Flock Backing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Three Phase 800mm Plate Fan. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avASTM Approved Popular Indoor Trampoline of Park(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Genuine Leather Credit Card Mens Wallet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Yitai Die Making 13mm Beech Rotary Plywood, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Classic Black Color Commercial Glass Door Multi Deck Refrigerator.Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Caseshows the peptides separations after enzymatic hydrolysis.Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBeverage Cooling Cube Ice Making/Ice Maker Machinery, but not shown in30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps,12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), and also free amino acids (Whitaker et al., 1963).Pneumatic Control Shuttle Valve NPT Thread. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator,1996).

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CONCLUSIONS

Lydite Fence Reel Reel with Extension Arms for Farm Fencing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application) for the financial support.

REFERENCES

1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sanneng Shoes Geniune Leather Safety Boots with Steel Toe Cap and Steel Midsole (SN5316)]

Brown, E. M; Thompson, C .J.,Customized 3D Medal for Religious Medal Gift, M.M.(1994),Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Nc Studio Control]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Manufacture Electromagnetic Impulse Solenoid Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Dbry-320 Christmas Stickers Labels Printing Machine. Analytical Biochemistry212,436-445[Manufacturer Plastic Injection Mould Molding Nylon PP Injection]

Dalev, P.G. and Simeonova, L.S.(1992),Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor. Biotechnology letters 14,6, 531-534[Stainless Steel Metric Female Flat Seat Hose Fittings]

Heidemann, E.(1997),Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.Leder 48,(5),99-104[Square Hot DIP Galvanized Tube for Steel Frame]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PVC Fabric Rapid Roller Shutter for Clearn Room]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Y32-200 Ton Wheelbarrow Edge Cutting and Curling Hydraulic Press Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)]

Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type]

Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve]

Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck, Part I.Aluminum Bright Polished Mirror Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Commercial Use Click Lock Standard Spc Vinyl Plank Flooring]

Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Motorcycle Reflector Injection Plastic Parts Mould Maker.O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W, 175-184[Hot-DIP Galvanized Steel Outdoor Flood Light Solar LED Street Lamp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler]

Whitaker,J.R.(1963),Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China. Analytical Chemistry35(12), 1950-1953[Aluminum Adjustable Quick Assemble Stage with Steps]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial Control Housing with Plate Shape (XD-CC04)Author for correspondence