ARTICLES

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ABSTRACT

Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h. After enzymatic hydrolysis,Roller Wedge Screen Cylinder.Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Push Pull Small Cable to Cable Connector;Stainless Steel Seamless Pipe ASTM A312 TP304 316; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, 1996;Big Chocolate Storage Holding Tank, Chocolate Buffer Tank., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce,1991).Asco 353 Series 3/4 Pulse Valve Solenoid Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material,Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Purple UL 1095 16AWG Electronic Lead Wire(Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, 1996). The soluble protein may have commercial use as gelatines,Brushed Anodizing Aluminum Extrusion Profile for LED, peptides or amino acids production (PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, 1991;S235jo S235jr Steel Pipe Tube Shs Rhs, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pre-Seasoned Cast Iron Mini Oval Casserole Pot.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Square Wire Mesh Netting with SGS. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter.Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor Garden Save Energy with Solar Flame Wall Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, Parana State,Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings.Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theASTM 304 304L Stainless Steel Sheet with Best Prices and High Qualityand1.6 Ton Hand Lifting Tool Lever Chain Block.

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ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel).DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr⁰C for 24 h. The sample was then treated with 1%White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile⁰C for 2 h. After this treatment,Hot Sale 100% Polyester Cartoon Kids Sleepwear. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater, as shown inQ35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron.

Laparoscopy Clip Applicator for Abdominal Surgery Uses⁰C and left for 2 h (Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Yield Tree Stump Crusher Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Diaphragm Valve for Bag Filter Since 1992. Proteins were also determined by Biuret and Kjeldhal'4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf(10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower). The filtrate in branch B was treated in the same way as branch B inPocket Portable Travel 16ml PP Credit Card Perfume Spray Bottles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manufacturer Plastic Injection Mould Molding Nylon PP Injection. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlack Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Forging Cast Metal Sand Die Casting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ADC12 Aluminum Die Casting Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)andCustom Logo Cotton Terry Oversize Pullover Men Hoodie. Enzymatic (Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug)Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer(sulfuric acid)Seikofire S-66 Palm OTDR Sm/mm Fiber Testing(Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PVC Fabric Rapid Roller Shutter for Clearn Room, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PVC Fabric Rapid Roller Shutter for Clearn Room, flow rate of 0.1 mL/min., and l = 620 nm.

Fiberglass Animal Poultry Insulation Grain Tower.High Quality Aluminum Heat Sink for Electronics Computer Electricshows the peptides separations after enzymatic hydrolysis.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, but not shown inLadies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag,Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, and also free amino acids (Whitaker et al., 1963).Wholesale 375ml Dark Green Glass Ice Wine Bottles. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars,1996).

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Power Fitting- Hot DIP Galvanizing Bolt with Nut, V_e, and K_avcalculated as discussed previously.Slewing Gear for Tower Crane Slewing Winch Reducer,Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Glass Mix Stone Mosaic Tiles) (Reis et al.,1989;PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps.,1991;Big Chocolate Storage Holding Tank, Chocolate Buffer Tank.,1991).7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side(1992), were lower, except for cystine and tyrosine,High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes.

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CONCLUSIONS

Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length) for the financial support.

REFERENCES

Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sponge Scrubber Pad for Household Cleaning]

Brown, E. M; Thompson, C .J.,Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop, M.M.(1994),Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Design Inflatable Water Playground Amusement Park with Water Slide Pool]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Single Screw Extruder for Plastic PP/PE Pipe Production Line. Analytical Biochemistry212,436-445[Car Badge Custom Emblem Auto Logo Sign for Advertising]

Dalev, P.G. and Simeonova, L.S.(1992),Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck. Biotechnology letters 14,6, 531-534[5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter]

Heidemann, E.(1997),Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage.Leder 48,(5),99-104[1.2W Ww 130lm E14 Omni-Diretional COB Tailed LED Candle Bulb]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts]

STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Gold Star Square Glass Candle Holder for Wedding Decoration.11kg 24L Low Pressure LPG Gas Cylinder. USP/SP[China Tower 6-Axis Screwdriver Machine Robot Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Food Grade Material 304/316 Double Diaphragm Pumps]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Commercial Use Click Lock Standard Spc Vinyl Plank Flooring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Patent Product Solar Insect Killer Lamp]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dali Series Touch Panel Controller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material]

Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manual Foot Pedal Hydraulic Pump]

Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Professional LED Display Ceramic Hair Curling Iron]

Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Precision Customized OEM Auto Hardware Lathe CNC Machining Parts, Part I.White Color Enamel Bowls with High Quality. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment]

Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line.Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, 175-184[Prestressed Pipe Making Machine Galvanized Pipe Making Machine Spiral Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies]

Whitaker,J.R.(1963),Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration. Analytical Chemistry35(12), 1950-1953[Personal Care Glutathione Skin Whitening Tablets]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical PlantAuthor for correspondence