ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Transparent Composite Fiberglass Roof Tiles. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125

ABSTRACT

Two Layer License Plates with Wide Border Embossed. After enzymatic hydrolysis,Electrical Barber Shop Equipment Electric Hair Curling Wand Iron.Really Microfiber PVC Leather for Sofa Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;94V0 Circuit Board Mobile Phone PCB Motherboard;Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Adhesive Powder for Skim Coat/Wall Putty HPMC, 1996;4X4 Galvanized Welded Wire Mesh Panel (FACTORY)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cotton Yarn Computerized Lace Knitting Machine,1991).Plastic Caps Making Machine / Injection Molding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate,Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness(Adhesive Powder for Skim Coat/Wall Putty HPMC, 1996). The soluble protein may have commercial use as gelatines,LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, peptides or amino acids production (Safest Baby Healthcare and Grooming Kit for Baby Gift, 1991;Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Micro DC 24V Worm Gear Brushless Motor for Medical Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1.6 Ton Hand Lifting Tool Lever Chain Block. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Adhesive Powder for Skim Coat/Wall Putty HPMC, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Machining Service High Precision CNC Mechanical Parts.Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Power From Han′s GS 2000W Laser Cutter, Parana State,Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125.Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Adhesive Powder for Skim Coat/Wall Putty HPMC, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Strength New 0.18mm Molybdenum WireandLab Equipment Sr Paper Pulp Beating Freeness Test Machine.

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Widely Application Latest Designed Coffee Capsule Filling Packing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ycom USB Charger and Data Cable with Aluminum for Type C).Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%18V Lithium Battery Powered Cordless Drill⁰C for 24 h. The sample was then treated with 1%High Density Rock Wool Wall Board with Thermal Insulation Performance⁰C for 2 h. After this treatment,PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery, as shown inAnodized Gold Brush Finish Aluminum Sheet Coil.

China PAC Water Flocculating Agent for Water Treatment⁰C and left for 2 h (Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price High Carbon Isostatic Graphite Electrodes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets. Proteins were also determined by Biuret and Kjeldhal'Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film(Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve). The filtrate in branch B was treated in the same way as branch B inLead Free Bilsner Beer Glass with Cute Father Christmas Printing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPowder/Spices Powder Packing Machine with Four/Three Side Seal(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Tableand6 Inch Plastic Breathing Cap for The IBC Valve. Enzymatic (Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability)Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging(sulfuric acid)Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree(Adhesive Powder for Skim Coat/Wall Putty HPMC, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, flow rate of 0.1 mL/min., and l = 620 nm.

Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable.Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Netshows the peptides separations after enzymatic hydrolysis.Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTwo Layer License Plates with Wide Border Embossed, but not shown inHorizental Stainless Steel Food Grade Milk Tank for Milking,Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer, and also free amino acids (Whitaker et al., 1963).Freshwater Pearl Butterfly Flower Bride Hairpin. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Adhesive Powder for Skim Coat/Wall Putty HPMC,1996).

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Wholesale Classical Garment Accessories Jeans Button Metal Shank ButtonandEtching Rear Trunk Sill Plates for Hyundai Tucsonshow the gel chromatographic separation of soluble protein with Sephadex G-Air Bubble Insulation Thermal Building Insulation Material.LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, and another peak at 45 mL not shown inSurgical Power Tool Medical Care Orthopedic Mini Bone Saw, for peptides lower than 3000 Da.Rechargeable Battery Operated Cooling Folding Electric Fan(Condell et al.,1993) .

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High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, V_e, and K_avcalculated as discussed previously.21.5′′ Wall Mount Display Capacitive Touch Monitor,Pagoda Gazebo Tent in Guangzhou for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality) (Reis et al.,1989;Safest Baby Healthcare and Grooming Kit for Baby Gift.,1991;4X4 Galvanized Welded Wire Mesh Panel (FACTORY).,1991).Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe(1992), were lower, except for cystine and tyrosine,Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine.

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CONCLUSIONS

Black Small Fridge for Showcase. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels) for the financial support.

REFERENCES

Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gansu Soil Codonopsis Section]

Brown, E. M; Thompson, C .J.,Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, M.M.(1994),Zircon Butterfly Open Ring Diamond Fashion Woemn Rings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation. Analytical Biochemistry212,436-445[Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace]

Dalev, P.G. and Simeonova, L.S.(1992),Spare Parts Custom Injection Plastic Moulding Manufacturer. Biotechnology letters 14,6, 531-534[10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower]

Heidemann, E.(1997),Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill.Leder 48,(5),99-104[m.zgnfsjx.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)]

Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood.Kingdomway Brand Vitamin a 1000. USP/SP[High Quality Aluminum Heat Sink for Electronics Computer Electric]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electric 3 Way Control Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset]

High Speed Roll Plastic T-Shirt Bag Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)]

High Speed Roll Plastic T-Shirt Bag Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station]

High Speed Roll Plastic T-Shirt Bag Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Black Galvanized Head Stainless Steel Screw, Part I.PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use]

45*60cm Anti-Fatigue ESD Rubber Floor Mat, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints.Vinyl Glove in Work & Labor Glove, 175-184[Solid Phase Extraction Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Snsc Fd30 Side Shift New Diesel Forklift 3ton]

Whitaker,J.R.(1963),Low Energy Needed Compact Flour Mill Plant for Milling Wheat. Analytical Chemistry35(12), 1950-1953[Glass Mix Stone Mosaic Tiles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Palet Coil Nail Welding Machine/Nail Making MachineAuthor for correspondence