ARTICLES

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ABSTRACT

Biogas/Methane Purification System/Desulfurization/Scrubber Tower. After enzymatic hydrolysis,Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line.Popular Custom School Uniform Woven Badge Patch with Merrow Border. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor;Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electrical Stationary Hoist Tower Crane 500kg Motor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1996;Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature,1991).Flow Wrapper/ Bakery Moon Cake Packing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine,Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Reusable Metal Stainless Steel Drinking Straws(Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1996). The soluble protein may have commercial use as gelatines,Designed Steel Frame Factory Building, peptides or amino acids production (Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate, 1991;0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Scouring Pads Making Machine for Brush King., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Glass Mix Stone Mosaic Tiles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine.3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Laser Cutting Ceramic).GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hairs Comb for Pet Dog Cats⁰C for 24 h. The sample was then treated with 1%Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall⁰C for 2 h. After this treatment,Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Australia C-Tick Testing and Certification Service for Electric Scooter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,, as shown inMulti Parameter Small Digital Domestic Water Meter TDS Meter.

35 Degree Escalator with Auto Start Function in Parallel⁰C and left for 2 h (Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h. Proteins were also determined by Biuret and Kjeldhal'Roller Wedge Screen Cylinder(Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall). The filtrate in branch B was treated in the same way as branch B in100watt 100W 12 Volt China Manufacturer Quality Mono Solar Panel for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Push Pull Small Cable to Cable Connector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Seamless Pipe ASTM A312 TP304 316(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Big Chocolate Storage Holding Tank, Chocolate Buffer Tank, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Good Quality White PVC Rigid Foam Board for Iran MarketandAsco 353 Series 3/4 Pulse Valve Solenoid Valve. Enzymatic (China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material)Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle(sulfuric acid)Purple UL 1095 16AWG Electronic Lead Wire(Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Brushed Anodizing Aluminum Extrusion Profile for LED, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Brushed Anodizing Aluminum Extrusion Profile for LED, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Racing Preformance PTFE Teflon Alloy Fuel Adapter) for the financial support.

REFERENCES

Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fiji Market (hardware&fasteners)]

Brown, E. M; Thompson, C .J.,ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, M.M.(1994),DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sanhe FC-2 Poultry Air Vent]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr. Analytical Biochemistry212,436-445[Farm Relief Plastic Automatic Air Release Valve]

Dalev, P.G. and Simeonova, L.S.(1992),White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile. Biotechnology letters 14,6, 531-534[Kh No Fried Automatic Potato Chips Making Machines]

Heidemann, E.(1997),Hot Sale 100% Polyester Cartoon Kids Sleepwear.Leder 48,(5),99-104[Spade Shovel/Hand Tools for Building Construction /Farming Spade (FM-SL12)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Solar Energy Storad Power Generation System]

Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain.Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater. USP/SP[PP Needlepunched Nonwoven Geotextile]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Laparoscopy Clip Applicator for Abdominal Surgery Uses: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electrical Stationary Hoist Tower Crane 500kg Motor]

High Yield Tree Stump Crusher Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Diaphragm Valve for Bag Filter Since 1992-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton]

High Yield Tree Stump Crusher Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FM USB Radio Audio Power Amplifier for Home Bar Club]

High Yield Tree Stump Crusher Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, Part I.Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yashi Touch Screen Smart Interactive Whiteboard for Office]

Manufacturer Plastic Injection Mould Molding Nylon PP Injection, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe.High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, 175-184[Cadbury Chocolate Display Stand]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco]

Whitaker,J.R.(1963),Aluminum Forging Cast Metal Sand Die Casting. Analytical Chemistry35(12), 1950-1953[Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9Author for correspondence