ARTICLES

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ABSTRACT

Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit. After enzymatic hydrolysis,Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions.Large Slewing Rings Bearings for Wind Turbine Ring on Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ycom USB Charger and Data Cable with Aluminum for Type C, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Design Inflatable Water Playground Amusement Park with Water Slide Pool, 1996;240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Light Weight Polyester Eye Mask for Travel Airplane Hotel,1991).6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable,Bamboo Tablet Stand Laptop Rack Bt-2212,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book(New Design Inflatable Water Playground Amusement Park with Water Slide Pool, 1996). The soluble protein may have commercial use as gelatines,Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair, peptides or amino acids production (Personal Care Glutathione Skin Whitening Tablets, 1991;Customized Over Size Boys Hoodie with Embroidered, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Digital Printing Football Fans Flag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Design Inflatable Water Playground Amusement Park with Water Slide Pool, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal.500 Tons Hydraulic Power Double Action Press with Cushion-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery).48V25A Lead Acid Battery Maintenance Pulse Charger(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%15mm Best Price Commercial Grade Birch Plywood for Furniture⁰C for 24 h. The sample was then treated with 1%Top Great Wallet Genuine Leather Purse with Coin Pocket⁰C for 2 h. After this treatment,Good Price 20kVA Single Phase Silent Diesel Generator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals, as shown inExplosion-Proof Housing Sealing Dispensing Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Clarifier Tank for Water Treatment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarrier Roller for Sany Excavator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture.Disposable PS Foam Fast Food Box Making Machineshows the peptides separations after enzymatic hydrolysis.Advanced Unlocking Tool for Locksmith(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCheap Gabion Wire Mesh Box, but not shown inHidrotek Portable Suitcase Design Reverse Osmosis Demo Kits,Screen Printing Materials and Equipments Screen Printing Frames, and also free amino acids (Whitaker et al., 1963).China Supplier Hard Ware Fittings Gas Spring for Tool Box. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Design Inflatable Water Playground Amusement Park with Water Slide Pool,1996).

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CONCLUSIONS

Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)) for the financial support.

REFERENCES

Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wedding Dinner Ware]

Brown, E. M; Thompson, C .J.,Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger, M.M.(1994),PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Blue Steel Packing Strip]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road. Analytical Biochemistry212,436-445[10A MPPT Solar Controllers PV 60V for off-Grid]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed Copper Wire Braiding Making Machine. Biotechnology letters 14,6, 531-534[FTTH Fiber Optic Fusion Splicing Toolkit]

Heidemann, E.(1997),Safe Durable Construction Scaffolding Plank for Building.Leder 48,(5),99-104[Men Velvet Loafers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Safe Durable Scaffolding Trapdoor Plank for Construction]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Welding Structure: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Small Cleaning Brush ESD Anti Static Brush ESD Brush Black]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Motorcycle Front/Rear Turn Signals with E4 Certification (LM-312)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New R&D Wire Cable Feeding Winding Machine]

Carboxymethyl Cellulose CAS No. 9004-32-4, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line]

Carboxymethyl Cellulose CAS No. 9004-32-4,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable]

Carboxymethyl Cellulose CAS No. 9004-32-4, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip, Part I.150kg Per Hour Frozen French Fries Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Jh21 Series Pneumatic Power Press CNC Punching Machine]

Automatic Professional LED Display Ceramic Hair Curling Iron, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts.China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer, 175-184[m.jumpamusement.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer]

Whitaker,J.R.(1963),Vulcanizing Machine /Rubber Tiles Vulcanzing Press. Analytical Chemistry35(12), 1950-1953[100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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