ARTICLES

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ABSTRACT

Picc Puncture Care Kit. After enzymatic hydrolysis,Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Temperaturethermocouple Male and Female Plug, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, 1996;Best Price Custom Embroidery Logo Hotel Slippers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent,1991).High Efficiency Fine Powder Vibrating Screener. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management,Plant Stands & Accessories Glass Hanging Flower Pot Bracket,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wooden Frame Stretched Canvas, Painting Canvas(34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, 1996). The soluble protein may have commercial use as gelatines,Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, peptides or amino acids production (SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, 1991;UF Membrane Aqu160 with Competitive Price and High Quality, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Atf-805 Auto Car Transmission System Flushing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.43 Glass Beads Cat Eye Aluminum Road Stud.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Precision C45 Steel CNC Lathe Parts.I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Transparent Composite Fiberglass Roof Tiles, Parana State,Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet.Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLd-Zg002 Special Corrosion and Scale Inhibitor for Chemical PlantandManufacture Best Choice Liquid Flocculant for Ink Removal.

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Two Layer License Plates with Wide Border Embossed(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door).Electrical Barber Shop Equipment Electric Hair Curling Wand Iron(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Really Microfiber PVC Leather for Sofa Furniture⁰C for 24 h. The sample was then treated with 1%94V0 Circuit Board Mobile Phone PCB Motherboard⁰C for 2 h. After this treatment,Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Adhesive Powder for Skim Coat/Wall Putty HPMC, as shown inProfessional Rope/Cable/Wire Braiding Machine with Factory Price.

4X4 Galvanized Welded Wire Mesh Panel (FACTORY)⁰C and left for 2 h (Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cotton Yarn Computerized Lace Knitting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Caps Making Machine / Injection Molding Machine. Proteins were also determined by Biuret and Kjeldhal'Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate(Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts). The filtrate in branch B was treated in the same way as branch B inm.cryspak.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Food Grade Material 304/316 Double Diaphragm Pumps. Jar Blowershows the peptides separations after enzymatic hydrolysis.Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in110/220V Handle Mild Steel Welding Machine PCB Board 160A, but not shown inHardware CNC Machinery Milling Part,FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator, and also free amino acids (Whitaker et al., 1963).High Power From Han′s GS 2000W Laser Cutter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts,1996).

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18V Lithium Battery Powered Cordless Drill, V_e, and K_avcalculated as discussed previously.High Density Rock Wool Wall Board with Thermal Insulation Performance,PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stand Hot and Cold Normal Water Dispenser with Refrigerator) (Reis et al.,1989;SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF.,1991;Best Price Custom Embroidery Logo Hotel Slippers.,1991).Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask(1992), were lower, except for cystine and tyrosine,China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery.

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CONCLUSIONS

China PAC Water Flocculating Agent for Water Treatment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Beverage Cooling Cube Ice Making/Ice Maker Machinery) for the financial support.

REFERENCES

Factory Price High Carbon Isostatic Graphite Electrodes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Small Batch CNC Machined and Plastic Camera Parts]

Brown, E. M; Thompson, C .J.,Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, M.M.(1994),Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film.The Journal of the American Leather Chemists Association (JALCA)89(7)[Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light. Analytical Biochemistry212,436-445[Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9. Biotechnology letters 14,6, 531-534[China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer]

Heidemann, E.(1997),Powder/Spices Powder Packing Machine with Four/Three Side Seal.Leder 48,(5),99-104[PVC Foam Sheet 20mm Thickness PVC Board Building Materials]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)]

Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Black Phosphated Bugle Head Csk Head Drywall Screw.Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine. USP/SP[ Leak Proof]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Simple Wiring Kit Cable Wire Harness Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6 Inch Plastic Breathing Cap for The IBC Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, Part I.Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange]

Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part.Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, 175-184[cnuneed.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart]

Whitaker,J.R.(1963),Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars. Analytical Chemistry35(12), 1950-1953[1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine PartsAuthor for correspondence