ARTICLES

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ABSTRACT

Stainless Steel Clarifier Tank for Water Treatment. After enzymatic hydrolysis,Carrier Roller for Sany Excavator.LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine;Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002), 1996;2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain,1991).Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dry Air Generator GF Series,Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Advanced Unlocking Tool for Locksmith(Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002), 1996). The soluble protein may have commercial use as gelatines,Screen Printing Materials and Equipments Screen Printing Frames, peptides or amino acids production (China Supplier Hard Ware Fittings Gas Spring for Tool Box, 1991;Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc.Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

AISI 201/202/301/304 Stainless Steel Welded Square PipeAlcalase2.5 L DX,Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle, Parana State,High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine.Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSmall Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)andBlack Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe.

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High Speed Copper Wire Braiding Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator).Safe Durable Construction Scaffolding Plank for Building(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Different Size of Ring NdFeB Magnet⁰C for 24 h. The sample was then treated with 1%Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine⁰C for 2 h. After this treatment,High Quality Welding Structure. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Carboxymethyl Cellulose CAS No. 9004-32-4's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher, as shown inEko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management.

Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip⁰C and left for 2 h (Mesh Bag Packing 20kg Pure White Garlic). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.150kg Per Hour Frozen French Fries Production Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Professional LED Display Ceramic Hair Curling Iron. Proteins were also determined by Biuret and Kjeldhal'CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts(New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet). The filtrate in branch B was treated in the same way as branch B inCnc Precision Turning Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vulcanizing Machine /Rubber Tiles Vulcanzing Press. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBelt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Omni Newest Manager Desk Office Smart Office Table Office Staff Table⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap, pH 3.25., mobile phase B: buffer solution of 0.01 mol/30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

304 Stainless Steel Anti-Corrosion Welded Wire MeshandPlastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage. Enzymatic (Full-Auto CNC Cutting Machine for Architecture Glass Sheet)Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product(sulfuric acid)Hot Forming Pushing Elbow Making Machine(Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, flow rate of 0.1 mL/min., and l = 620 nm.

Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner.Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardwareshows the peptides separations after enzymatic hydrolysis.As568 Standard Brown FKM Rubber O Ring for Sealing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectric Heavy Duty Rail Guided Vehicle for Transportation, but not shown inDesktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24,The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent, and also free amino acids (Whitaker et al., 1963).Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002),1996).

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Bathroom False Plastic Board Interior Decorative 3D Wall Panels, V_e, and K_avcalculated as discussed previously.Mushroom Head Stud Sert,Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall) (Reis et al.,1989;China Supplier Hard Ware Fittings Gas Spring for Tool Box.,1991;2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle.,1991).Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket(1992), were lower, except for cystine and tyrosine,High Borosilicate Glass Baking Dish Set Clear.

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CONCLUSIONS

G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss) for the financial support.

REFERENCES

Embossing Roller for Tissue Paper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.cimmbeatrice.com]

Brown, E. M; Thompson, C .J.,Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel, M.M.(1994),Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates.The Journal of the American Leather Chemists Association (JALCA)89(7)[Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[SMD LED Light-Operated USB Rechargeble Flashlight]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Colorful  Reflective Sticker Reflective Tape. Analytical Biochemistry212,436-445[Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)]

Dalev, P.G. and Simeonova, L.S.(1992),Concrete Spare Part Electrical System Central Distribution Box. Biotechnology letters 14,6, 531-534[Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine]

Heidemann, E.(1997),Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress.Leder 48,(5),99-104[Dust Hood Fume Extraction Systems for Robotic Laser Cutting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Non Stick Silicone Coated Fiberglass Cloth]

Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),50Hz to 60Hz Frequency Inverter for Water Pump.Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330). USP/SP[40A C Electronic Air Circuit Breaker with Dz47-4p]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mold Injection Molding Plastic Products White PC Housing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3*19.8 NRA G2 AISI420 Needle Roller for Bearing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Fasteners]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker]

4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[500 Tons Hydraulic Power Double Action Press with Cushion]

4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sanhe FC-2 Poultry Air Vent]

4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVC Cabinet Construction Board, White 18mm PVC Foam Board, Part I.2019 New Portable Mini Dental X-ray Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

Ss Reduced Port 2000wog 1PC Screwed Ball Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous.China Prefab Steel Building for Garment Factory, 175-184[Acetic Acid Empirical Formula]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Farm Relief Plastic Automatic Air Release Valve]

Whitaker,J.R.(1963),Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile. Analytical Chemistry35(12), 1950-1953[Kh No Fried Automatic Potato Chips Making Machines]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Solar Energy Storad Power Generation SystemAuthor for correspondence