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ABSTRACT

100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale. After enzymatic hydrolysis,Carbon Graphite Crucible.Decorative Aluminum Solid Panel for Commercial Building. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Magic Hair Twist   Curl Sponge Brush, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75), 1996;Geyi Disposable Laparoscopic Visible Optical Trocar., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding,1991).Bimetallic Thermometer Industrial Temperature Gauge. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,19 Network Cabinet with Lockable Rear Door,Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,175mm Diamond Cutting Blade for Granite&Tiles(Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75), 1996). The soluble protein may have commercial use as gelatines,Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, peptides or amino acids production (PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, 1991;Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Uprigth Touch-Screen Red Wine Chiller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery.Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Groove Type Wood Log Tree Peeling and Debarking Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, Parana State,Galvanized Roller Shutter Window Torsion Springs.Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the14dBi 470-790MHz Outdoor Digital UHF VHF TV AntennaandBluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring.

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Custom Striped Compression Soccer Socks for Adults(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Machining Non-Standard Hardware Parts).Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen⁰C for 24 h. The sample was then treated with 1%High Tensile Strength Galvanized Steel Wire⁰C for 2 h. After this treatment,24 Volt Fire Alarm Strobe Light Horn Strobe Sounder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Price PVC Coated Fiberglass Mesh Insect Window Screen, as shown inExport Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce.

Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application⁰C and left for 2 h (Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory OEM CNC Machined Gear Motor Shaft. Proteins were also determined by Biuret and Kjeldhal'High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage(Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line). The filtrate in branch B was treated in the same way as branch B inEmbossed Foil Aluminium Foil Jumbo Roll. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pink French Loop Terry Knit Fabric for Jacket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDie Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Three Phase 800mm Plate Fan.Classical New Coming Plastic Female Sunglassesshows the peptides separations after enzymatic hydrolysis.ASTM Approved Popular Indoor Trampoline of Park(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSx Sm Fiber Optic MPO to MPO Adapter with Flange Type, but not shown inRotary Vibrating Screen/Sieve/Sifter/Griddle Machine,Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building, and also free amino acids (Whitaker et al., 1963).Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75),1996).

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CONCLUSIONS

Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Parcel Tape Adhesive OPP Packing Tape) for the financial support.

REFERENCES

12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Pans Forming Machine]

Brown, E. M; Thompson, C .J.,Pneumatic Control Shuttle Valve NPT Thread, M.M.(1994),Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing.The Journal of the American Leather Chemists Association (JALCA)89(7)[2 Round Sash Pulley for Chain - Brass (PVD)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized 8 Layers Round LED Panel Aluminum PCB for LED Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-OEM 15PCS Electric Face Makeup Brush with Ce Certificate. Analytical Biochemistry212,436-445[33kv Dry Type Resin Casted Distribution Transformer]

Dalev, P.G. and Simeonova, L.S.(1992),New Styles Fashion Erotic Women Underwear Sexy Lingerie. Biotechnology letters 14,6, 531-534[Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Festival Ceramic Mug]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fly]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Diy 1000w Fiber Laser Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine]

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Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Refrigeration Digital Temperature Controller Stc-100A]

Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, Part I.Customized 3D Medal for Religious Medal Gift. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss]

Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dbry-320 Christmas Stickers Labels Printing Machine.Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, 175-184[m.jssintering.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank]

Whitaker,J.R.(1963),Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor. Analytical Chemistry35(12), 1950-1953[Good Prize Solar PV Mounting Panel System for Ground]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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