ARTICLES

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ABSTRACT

Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair. After enzymatic hydrolysis,Round Shape Aluminum PCB and PCBA.36PCS LED Gold Matrix Stage Zoom Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.110/220V Handle Mild Steel Welding Machine PCB Board 160A, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, 1996;Enamel Cast Iron Mini Casserole Cast Iron Cocotte., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part,1991).Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug,Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC System Air Duct Line5 Make Machine for HVAC(Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, 1996). The soluble protein may have commercial use as gelatines,Titanium Dioxide Anatase for Paper Making, peptides or amino acids production (40A C Electronic Air Circuit Breaker with Dz47-4p, 1991;Pipe Thermostat with Sensor Ce Certificate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Walking Long Distance Surveying Instrument Meter Measuring Wheel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chinese Supplier of PVC Profile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tianjin Manufacturer PTFE Coated Butterfly Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard.Wholesale Prices of 20W 40W 60W Solar Street Lights-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sinicline Custom Brand with Plastic Pouch Hangtag for Hats(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pet Anti-Static Needled Mat(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling).Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Sheet Metal Fabrication for Aluminum Parts⁰C for 24 h. The sample was then treated with 1%Hwf Tsp Core Bit for Geotechnical Drilling⁰C for 2 h. After this treatment,Price of Automatic Sludge Dewatering Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Worm Gear Operation Wafer Lined F3 Butterfly Valve, as shown inZircon Butterfly Open Ring Diamond Fashion Woemn Rings.

Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad⁰C and left for 2 h (Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Positive Drive Turn Belt for Spiral Freezing Equipment. Proteins were also determined by Biuret and Kjeldhal'Chinese Herb Medicine Angelica Root Extract(Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa). The filtrate in branch B was treated in the same way as branch B inSynthetic Graphite. The final soluble protein shown were used for peptides separations using gel permeation chromatography,S Series China Generator Helical-Worm Crushers Reduction Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOriginal Testo 174t Mini Temperature Data Logger No. 0572 1560(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Whole Set Gas Spring Strut for Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

60ml 100ml Customized Glass Jar for SpiceandDia. 5.5mm Bonding ND: YAG Laser Rod Lens. Enzymatic (CNC Machining Turning Anodized Aluminum Worm Gear)China Solar Panel Solar Cell Photovoltaic 300W Hot Sell(sulfuric acid)Vacuum Coating Machine Water Chiller (CW-6300AN)(Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Planter Moving Round Planter Saucer Casters Plant Trolley (M), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Planter Moving Round Planter Saucer Casters Plant Trolley (M), flow rate of 0.1 mL/min., and l = 620 nm.

1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China.Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agentshows the peptides separations after enzymatic hydrolysis.Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHumidity Refrigeration Digital Controller Jsd-100+, but not shown in6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8),Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts, and also free amino acids (Whitaker et al., 1963).CNC Machining Non-Standard Hardware Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming,1996).

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Water Meter Spare Parts Rubber Rings for Sealing, V_e, and K_avcalculated as discussed previously.Stainless Steel HK Series Needle Roller Bearing HK0408,Road Construction Fabric Non-Woven Geotextile 200GSM. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Chinese Supplier of PVC Profile) (Reis et al.,1989;40A C Electronic Air Circuit Breaker with Dz47-4p.,1991;Enamel Cast Iron Mini Casserole Cast Iron Cocotte.,1991).High Polymer Modified Cement Based Elastic Waterproof Coating(1992), were lower, except for cystine and tyrosine,Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe.

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CONCLUSIONS

Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)) for the financial support.

REFERENCES

Fashion Shoulder Backpack Handbag School Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Anodizing Dyes For Aluminum Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity, M.M.(1994),Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-FTTH Fiber Optic Fusion Splicing Toolkit. Analytical Biochemistry212,436-445[Professional Bb Trumpet (ATR6506)]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed Cable Wire Winding Braiding Machine. Biotechnology letters 14,6, 531-534[Vacuum Coating Machine Water Chiller (CW-6300AN)]

Heidemann, E.(1997),22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill.Leder 48,(5),99-104[High Quality Disposable Lancet / Blood Collection Needle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Home Machine 2019 New Commercial Treadmill Gym Equipment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[DOXIN 12/24V 220V 150W Outdoor Pure Sine Wave Inverter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine]

Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Residential Reverse Osmosis 0.0001um Water Filter Water Filtration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine]

Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt]

Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle, Part I.China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Christmas Decoration Rose Flower LED Light String]

Folding Round Picnic Caravan Table RV Picnic Table Camping Table, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits.3HP Industrial Cooling System Water Cooled Chiller, 175-184[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Laundry Ozone Generator for Jeans]

Whitaker,J.R.(1963),OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress. Analytical Chemistry35(12), 1950-1953[Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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