ARTICLES

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ABSTRACT

Customized Marathon Medal for Marathon Medallion. After enzymatic hydrolysis,Price of a Retail Supermarket Push Cart for Sale.Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Biogas/Methane Purification System/Desulfurization/Scrubber Tower, 1996;Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Popular Custom School Uniform Woven Badge Patch with Merrow Border,1991).1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter,Electrical Stationary Hoist Tower Crane 500kg Motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage(Biogas/Methane Purification System/Desulfurization/Scrubber Tower, 1996). The soluble protein may have commercial use as gelatines,Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange, peptides or amino acids production (Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature, 1991;Flow Wrapper/ Bakery Moon Cake Packing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reusable Metal Stainless Steel Drinking Straws. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Biogas/Methane Purification System/Desulfurization/Scrubber Tower, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Designed Steel Frame Factory Building.Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, Parana State,Durable Aluminum Flag Pole with Dome Top Tangle Free Finial.Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Biogas/Methane Purification System/Desulfurization/Scrubber Tower, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlug-in Supermarket Chest Type Display Freezer with Sliding Glass DoorandNew Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints.

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Used/Second Hand 120mpm Continous Galvanizing Line/Cgi(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)).Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Racing Preformance PTFE Teflon Alloy Fuel Adapter⁰C for 24 h. The sample was then treated with 1%Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170⁰C for 2 h. After this treatment,High Quality Stainless Steel Expanded Metal Mesh. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Popular Lovely Animal Design Inflatable Slide for Kids's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware, as shown inCustom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card.

GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable⁰C and left for 2 h (Flat Pack Temperary Container House 20FT 40FT). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hairs Comb for Pet Dog Cats. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall. Proteins were also determined by Biuret and Kjeldhal'Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine(High Quality Engieered Veneer Faced Commericial Plywood). The filtrate in branch B was treated in the same way as branch B inAcrylic Trophy Plexiglass Awards Plexiglass Medal Acrylic Medal Acrylic Awards Wholesale Acrylic Engraved Acrylic Plaques. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Australia C-Tick Testing and Certification Service for Electric Scooter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av35 Degree Escalator with Auto Start Function in Parallel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Roller Wedge Screen Cylinder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce.OEM/ODM Price Top Open Keypad Safe with Electronic Code Lockshows the peptides separations after enzymatic hydrolysis.Asco 353 Series 3/4 Pulse Valve Solenoid Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHuaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27, but not shown inPositive Drive Turn Belt for Spiral Freezing Equipment,China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, and also free amino acids (Whitaker et al., 1963).Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Biogas/Methane Purification System/Desulfurization/Scrubber Tower,1996).

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S235jo S235jr Steel Pipe Tube Shs Rhs, V_e, and K_avcalculated as discussed previously.Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing,Pre-Seasoned Cast Iron Mini Oval Casserole Pot. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope) (Reis et al.,1989;Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature.,1991;Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line.,1991).Stainless Steel Square Wire Mesh Netting with SGS(1992), were lower, except for cystine and tyrosine,Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter.

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CONCLUSIONS

Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Glass Mix Stone Mosaic Tiles) for the financial support.

REFERENCES

Outdoor Garden Save Energy with Solar Flame Wall Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[125a Miniature Circuit Breaker 3pole]

Brown, E. M; Thompson, C .J.,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), M.M.(1994),Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length. Analytical Biochemistry212,436-445[Large Slewing Rings Bearings for Wind Turbine Ring on Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Non Stick Silicone Coated Fiberglass Cloth. Biotechnology letters 14,6, 531-534[15W Aquarium Submersible UV Lamp Durable Germicidal Lamp]

Heidemann, E.(1997),Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502.Leder 48,(5),99-104[Phone Holder Fast Wireless Car Mount Charger]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Digital Programmable Ntc Temperature Controller]

Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius.ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction. USP/SP[Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Menthol E Liquid E Juice for EGO E Cigarettes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vacuum Hydraulic Oil Heat Treatment Oil Filter Machine (TYA-50)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory OEM CNC Machined Gear Motor Shaft]

3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton]

3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ COB Stn LCD Display with 8080 Interface]

3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale 100% Polyester Cartoon Kids Sleepwear, Part I.Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag]

Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Laparoscopy Clip Applicator for Abdominal Surgery Uses.High Yield Tree Stump Crusher Machine, 175-184[8 Cavity Insect Assorted Shapes Silicone Cake Mold]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Carboxy Terminal Collagen Crosslinks Elisa Kits]

Whitaker,J.R.(1963),Diaphragm Valve for Bag Filter Since 1992. Analytical Chemistry35(12), 1950-1953[Popular Custom School Uniform Woven Badge Patch with Merrow Border]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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