ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Chinese Galvanized Rectangular Tube for Buildings Materials. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Customized Chilled Cast Iron Roll

ABSTRACT

IMTEK High Precision and Rigidity Linear Guides for CNC machinery. After enzymatic hydrolysis,Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask.Yashi Touch Screen Smart Interactive Whiteboard for Office. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electric Fireplace PCBA with Double Layers Circuit Board;China Shipping Logistics to Stockholm; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1996;Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Animal Agricultural Damping Horse Stall Rubber Cow Mat,1991).CNC Automatic Laser Wood Cutting Machine Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Yc Pure White Nylon Powder for 3D Printing,Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM(Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1996). The soluble protein may have commercial use as gelatines,High Quality Liquid Spouted Stand up Pouch with Custom Logo, peptides or amino acids production (Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container, 1991;Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Modern Pharmacy Vacuum Filter Press., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100% Cotton Luxury Printed Bedding Comforter Set.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mouse Pad with Gel Wrist Rest. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Size Christmas Gift Personalized Watch Boxes for Men.Best Selling Quality Aluminum Acetylene Gas Cylinder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Coal Mine Mini Electric Excavator with Quick Coupling(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Direct Sale Single Cloth Hanger Hook, Parana State,China Customized Chilled Cast Iron Roll.2 Round Sash Pulley for Chain - Brass (PVD), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine PartsandCE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).

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Anodized Aluminum Expanded Metal Mesh Competitive Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden).Hot DIP Galvanized G2150 D Ring Shackle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair⁰C for 24 h. The sample was then treated with 1%Round Shape Aluminum PCB and PCBA⁰C for 2 h. After this treatment,36PCS LED Gold Matrix Stage Zoom Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chicken Cage Mesh Hexagonal Wire Mesh Machine, as shown inCustomized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door.

110/220V Handle Mild Steel Welding Machine PCB Board 160A⁰C and left for 2 h (Mass Stock Yl10.2 Carbide Rods for Milling 45HRC). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Enamel Cast Iron Mini Casserole Cast Iron Cocotte. Proteins were also determined by Biuret and Kjeldhal'Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part(Size 000 Clear Pullulan Vegetarian Capsules). The filtrate in branch B was treated in the same way as branch B inHigh Hardness Nylon Sheet with Wear-Resistance. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Cashew Processing Cashew Nuts Shelling Peeling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Titanium Dioxide Anatase for Paper Making⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/40A C Electronic Air Circuit Breaker with Dz47-4p, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pipe Thermostat with Sensor Ce Certificate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sinicline Custom Brand with Plastic Pouch Hangtag for Hats.Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Saleshows the peptides separations after enzymatic hydrolysis.Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSelf Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, but not shown inFerrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh,Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag, and also free amino acids (Whitaker et al., 1963).VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings,1996).

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Pet Anti-Static Needled Mat, V_e, and K_avcalculated as discussed previously.Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine,OEM Sheet Metal Fabrication for Aluminum Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Professional Factory Supply Design Cat Tree Pet Product) (Reis et al.,1989;Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container.,1991;Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#.,1991).Hwf Tsp Core Bit for Geotechnical Drilling(1992), were lower, except for cystine and tyrosine,Price of Automatic Sludge Dewatering Machine.

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CONCLUSIONS

Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory) for the financial support.

REFERENCES

Worm Gear Operation Wafer Lined F3 Butterfly Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hq25 2X2500W RMS 60V-265V Professional Power Amplifier]

Brown, E. M; Thompson, C .J.,Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad, M.M.(1994),Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Home Products 16′ Perforated Flex Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Positive Drive Turn Belt for Spiral Freezing Equipment. Analytical Biochemistry212,436-445[Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608]

Dalev, P.G. and Simeonova, L.S.(1992),Chinese Herb Medicine Angelica Root Extract. Biotechnology letters 14,6, 531-534[Groove Type Wood Log Tree Peeling and Debarking Machine]

Heidemann, E.(1997),S Series China Generator Helical-Worm Crushers Reduction Box.Leder 48,(5),99-104[www.mybarberchairs.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hex Thin Head Nut]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[SAA Approved Australian Standard Two or Three Core 0.75mm2 Black Plug Power Cord]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Anodized Aluminum Expanded Metal Mesh Competitive Price]

Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Whole Set Gas Spring Strut for Furniture, Part I.Dia. 5.5mm Bonding ND: YAG Laser Rod Lens. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DC24V 36W Solar Fan Motor PCBA Card]

CNC Machining Turning Anodized Aluminum Worm Gear, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Solar Panel Solar Cell Photovoltaic 300W Hot Sell.Vacuum Coating Machine Water Chiller (CW-6300AN), 175-184[Hydraulic Cylinder For Agriculture Trailer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Toilet Roll Tissue Wrapping Machine with Auto - Counting]

Whitaker,J.R.(1963),Planter Moving Round Planter Saucer Casters Plant Trolley (M). Analytical Chemistry35(12), 1950-1953[ Painting Canvas]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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