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ABSTRACT

PVC Fabric Rapid Roller Shutter for Clearn Room. After enzymatic hydrolysis,Fiberglass Animal Poultry Insulation Grain Tower.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, 1996;Cog Graphic FPC LCD Display Module., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Power Fitting- Hot DIP Galvanizing Bolt with Nut,1991).Slewing Gear for Tower Crane Slewing Winch Reducer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine,7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes(Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, 1996). The soluble protein may have commercial use as gelatines,Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System, peptides or amino acids production (Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8), 1991;Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Single Screw Extruder for Plastic PP/PE Pipe Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage.Gold Star Square Glass Candle Holder for Wedding Decoration-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:11kg 24L Low Pressure LPG Gas Cylinder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cabinet Shelf Support Pin Furniture Hardware Fittings⁰C and left for 2 h (500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reasonable Price High Quality Nylon Cable Gland. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan. Proteins were also determined by Biuret and Kjeldhal'Disposable Anesthesia Breathing Circuit Tube( Laser Cutting Ceramic). The filtrate in branch B was treated in the same way as branch B inTractor Mounted Small Ear Corn/Maize Combine Harvester Prices. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Counter-Clip Fluorine-Lined Butterfly Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/DC Brushless Motor for CNC Machine with ISO9001, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng) for the financial support.

REFERENCES

Portable Telescopic Straw Metal Drinking Straw with Brush Case, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Top Selling Fused Silica Powder Sio2 99.9%]

Brown, E. M; Thompson, C .J.,Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware, M.M.(1994),Etching Rear Trunk Sill Plates for Hyundai Tucson.The Journal of the American Leather Chemists Association (JALCA)89(7)[3*19.8 NRA G2 AISI420 Needle Roller for Bearing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163. Analytical Biochemistry212,436-445[500 Tons Hydraulic Power Double Action Press with Cushion]

Dalev, P.G. and Simeonova, L.S.(1992),Lab Equipment Sr Paper Pulp Beating Freeness Test Machine. Biotechnology letters 14,6, 531-534[Sanhe FC-2 Poultry Air Vent]

Heidemann, E.(1997),CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy.Leder 48,(5),99-104[High Speed Low Rpm 16mm Micro DC Gear Motor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Kh No Fried Automatic Potato Chips Making Machines]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Solar Energy Storad Power Generation System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Anon Agricultural Wheel Transplanter Riding Rice Transplanter-Walking Type 6 Rows]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PP Needlepunched Nonwoven Geotextile]

Double Roller Rotary Wood Peeling Machine with CE for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Double Roller Rotary Wood Peeling Machine with CE for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)]

Double Roller Rotary Wood Peeling Machine with CE for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), Part I.Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electrical Stationary Hoist Tower Crane 500kg Motor]

Ladle Refining Melting Steel Furnace Casting Ladle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lishui Factory High Quality Metal Rolling Ball Screw.Y-Spring Threaded Check Valve, 800 Wog, 175-184[Pen Type LC Mu One Click Fiber Optic 1.25mm Connector Cleaner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton]

Whitaker,J.R.(1963),OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part. Analytical Chemistry35(12), 1950-1953[FM USB Radio Audio Power Amplifier for Home Bar Club]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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