ARTICLES

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ABSTRACT

Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange. After enzymatic hydrolysis,Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature.Flow Wrapper/ Bakery Moon Cake Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine;Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reusable Metal Stainless Steel Drinking Straws, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Designed Steel Frame Factory Building, 1996;Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table,1991).Scouring Pads Making Machine for Brush King. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor,Glass Mix Stone Mosaic Tiles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine(Designed Steel Frame Factory Building, 1996). The soluble protein may have commercial use as gelatines,3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, peptides or amino acids production (Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, 1991;Used/Second Hand 120mpm Continous Galvanizing Line/Cgi, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Racing Preformance PTFE Teflon Alloy Fuel Adapter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Designed Steel Frame Factory Building, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Stainless Steel Expanded Metal Mesh.Popular Lovely Animal Design Inflatable Slide for Kids-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

GYXTW Sm 9/125 Armored Fiber Optic Outdoor CableAlcalase2.5 L DX,Hairs Comb for Pet Dog Cats, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Australia C-Tick Testing and Certification Service for Electric Scooter, Parana State,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Designed Steel Frame Factory Building, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting PartandHigh Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover.

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35 Degree Escalator with Auto Start Function in Parallel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Air Cooled Split Type Ducted Air Conditioner AC Unit).Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h⁰C for 24 h. The sample was then treated with 1%Roller Wedge Screen Cylinder⁰C for 2 h. After this treatment,Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Push Pull Small Cable to Cable Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Seamless Pipe ASTM A312 TP304 316, as shown inFull Automatic 4000b/h 500ml Plastic Jar Making Machine.

Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco⁰C and left for 2 h (Made in China Lqa Strap Banding Tool). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Big Chocolate Storage Holding Tank, Chocolate Buffer Tank. Proteins were also determined by Biuret and Kjeldhal'Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce(Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application). The filtrate in branch B was treated in the same way as branch B inBlack Leather Ergonomic Office Chair Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Asco 353 Series 3/4 Pulse Valve Solenoid Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Brushed Anodizing Aluminum Extrusion Profile for LED⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, pH 3.25., mobile phase B: buffer solution of 0.01 mol/S235jo S235jr Steel Pipe Tube Shs Rhs, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Modern Pharmacy Vacuum Filter PressandSeamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing. Enzymatic (Pre-Seasoned Cast Iron Mini Oval Casserole Pot)Stainless Steel Square Wire Mesh Netting with SGS(sulfuric acid)Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter(Designed Steel Frame Factory Building, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, flow rate of 0.1 mL/min., and l = 620 nm.

Outdoor Garden Save Energy with Solar Flame Wall Light.Folding Play Mat Crawl Play Matshows the peptides separations after enzymatic hydrolysis.CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDbry-320 Christmas Stickers Labels Printing Machine, but not shown inHigh Density Celuca Kitchen Cabinets PVC Foam Board,Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag, and also free amino acids (Whitaker et al., 1963).Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Designed Steel Frame Factory Building,1996).

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ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, V_e, and K_avcalculated as discussed previously.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge,3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Robot of World Robotics Competition) (Reis et al.,1989;Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net.,1991;Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate.,1991).White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile(1992), were lower, except for cystine and tyrosine,Hot Sale 100% Polyester Cartoon Kids Sleepwear.

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CONCLUSIONS

Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall) for the financial support.

REFERENCES

Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Beauty Luxury Matte Color Matte Lipstick]

Brown, E. M; Thompson, C .J.,Laparoscopy Clip Applicator for Abdominal Surgery Uses, M.M.(1994),High Yield Tree Stump Crusher Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Powder and Wax Brush with Wooden Handle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Diaphragm Valve for Bag Filter Since 1992. Analytical Biochemistry212,436-445[FRP Water Tank GRP Fishing Tanks From Factory Supply]

Dalev, P.G. and Simeonova, L.S.(1992),4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf. Biotechnology letters 14,6, 531-534[China Solar Panel Solar Cell Photovoltaic 300W Hot Sell]

Heidemann, E.(1997),Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine.Leder 48,(5),99-104[80W Portable Solar Panel Kit with Battery Clamp for Car]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855]

Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Manufacturer Plastic Injection Mould Molding Nylon PP Injection.Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe. USP/SP[Waterproof Covering Material Interior Wall Composite Panel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Precision Piston Ring Good Shape Ability High Quality Surface]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum PCB Circuit Board SMD PCBA LED PCB]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Solar Powered Agriculture Insects Pest Killer Lamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software]

Aluminum Forging Cast Metal Sand Die Casting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ADC12 Aluminum Die Casting Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping]

Aluminum Forging Cast Metal Sand Die Casting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Refrigeration Digital Thermometer]

Aluminum Forging Cast Metal Sand Die Casting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, Part I.Custom Logo Cotton Terry Oversize Pullover Men Hoodie. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Residence Use 220V 50Hz 12kw Portable silent Diesel Generator]

Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer.Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, 175-184[www.citystreetlighting.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Strength New 0.18mm Molybdenum Wire]

Whitaker,J.R.(1963),PVC Fabric Rapid Roller Shutter for Clearn Room. Analytical Chemistry35(12), 1950-1953[Lab Equipment Sr Paper Pulp Beating Freeness Test Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Disposable Precision Infusion Set with Filter and Needle for Single UseAuthor for correspondence