ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Factory Direct Sale Single Cloth Hanger Hook. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2 Round Sash Pulley for Chain - Brass (PVD)

ABSTRACT

Anodized Aluminum Expanded Metal Mesh Competitive Price. After enzymatic hydrolysis,Hot DIP Galvanized G2150 D Ring Shackle.Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Round Shape Aluminum PCB and PCBA;36PCS LED Gold Matrix Stage Zoom Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chicken Cage Mesh Hexagonal Wire Mesh Machine, 1996;110/220V Handle Mild Steel Welding Machine PCB Board 160A., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming,1991).Enamel Cast Iron Mini Casserole Cast Iron Cocotte. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part,Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug(Chicken Cage Mesh Hexagonal Wire Mesh Machine, 1996). The soluble protein may have commercial use as gelatines,Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine, peptides or amino acids production (CNC System Air Duct Line5 Make Machine for HVAC, 1991;Titanium Dioxide Anatase for Paper Making, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;40A C Electronic Air Circuit Breaker with Dz47-4p., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pipe Thermostat with Sensor Ce Certificate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Walking Long Distance Surveying Instrument Meter Measuring Wheel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chicken Cage Mesh Hexagonal Wire Mesh Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chinese Supplier of PVC Profile.Tianjin Manufacturer PTFE Coated Butterfly Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A), Parana State,2 Round Sash Pulley for Chain - Brass (PVD).ALS Stage 5W RGB Animation Laser Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Chicken Cage Mesh Hexagonal Wire Mesh Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePalet Coil Nail Welding Machine/Nail Making MachineandFashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby.

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Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut).Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pet Anti-Static Needled Mat⁰C for 24 h. The sample was then treated with 1%Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine⁰C for 2 h. After this treatment,OEM Sheet Metal Fabrication for Aluminum Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hwf Tsp Core Bit for Geotechnical Drilling's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Price of Automatic Sludge Dewatering Machine, as shown inCar Badge Custom Emblem Auto Logo Sign for Advertising.

Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine⁰C and left for 2 h (5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Worm Gear Operation Wafer Lined F3 Butterfly Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad. Proteins were also determined by Biuret and Kjeldhal'Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag(Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts). The filtrate in branch B was treated in the same way as branch B inHospital Use First Aid Cabinet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Positive Drive Turn Belt for Spiral Freezing Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chinese Herb Medicine Angelica Root Extract. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avS Series China Generator Helical-Worm Crushers Reduction Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Original Testo 174t Mini Temperature Data Logger No. 0572 1560⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Vacuum Coating Machine Water Chiller (CW-6300AN).Food Grade Material 304/316 Double Diaphragm Pumpsshows the peptides separations after enzymatic hydrolysis.Planter Moving Round Planter Saucer Casters Plant Trolley (M)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPatent Product Solar Insect Killer Lamp, but not shown inPE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material,1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China, and also free amino acids (Whitaker et al., 1963).Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chicken Cage Mesh Hexagonal Wire Mesh Machine,1996).

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Automatic Professional LED Display Ceramic Hair Curling Ironand2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatmentshow the gel chromatographic separation of soluble protein with Sephadex G-Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts.CNC Machining Non-Standard Hardware Parts, and another peak at 45 mL not shown inWomen Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies, for peptides lower than 3000 Da.DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb(Condell et al.,1993) .

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Galvanized Solar System Mounting Bracket Connector, V_e, and K_avcalculated as discussed previously.Digital Programmable Ntc Temperature Controller,Water Meter Spare Parts Rubber Rings for Sealing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant) (Reis et al.,1989;CNC System Air Duct Line5 Make Machine for HVAC.,1991;110/220V Handle Mild Steel Welding Machine PCB Board 160A.,1991).Stainless Steel HK Series Needle Roller Bearing HK0408(1992), were lower, except for cystine and tyrosine,Road Construction Fabric Non-Woven Geotextile 200GSM.

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CONCLUSIONS

High Polymer Modified Cement Based Elastic Waterproof Coating. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset, M.M.(1994),Fashion Shoulder Backpack Handbag School Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Rope/Cable/Wire Braiding Machine with Factory Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity. Analytical Biochemistry212,436-445[Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl. Biotechnology letters 14,6, 531-534[Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts]

Heidemann, E.(1997),FTTH Fiber Optic Fusion Splicing Toolkit.Leder 48,(5),99-104[FITTINGS SERIES]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Long Distance Measurement Power Laser Digital Meter]

Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Speed Cable Wire Winding Braiding Machine.22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill. USP/SP[ Jar Blower]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[110/220V Handle Mild Steel Welding Machine PCB Board 160A]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Temperature Alternative Stainless Steel Band in Different Package: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hardware CNC Machinery Milling Part]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Unfolding Grocery Push Carts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058]

Water Cooled Low Temperature Screw Chiller for Industrial Workshop, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Home Machine 2019 New Commercial Treadmill Gym Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT]

Water Cooled Low Temperature Screw Chiller for Industrial Workshop,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Laundry Used Gas Water Heater with CE/Asme Certificate]

Water Cooled Low Temperature Screw Chiller for Industrial Workshop, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card, Part I.Residential Reverse Osmosis 0.0001um Water Filter Water Filtration. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle]

Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses.Folding Round Picnic Caravan Table RV Picnic Table Camping Table, 175-184[mop-x.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2.0mm Yellow Markers for Fiber Optic Multi Patch Cord]

Whitaker,J.R.(1963),Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits. Analytical Chemistry35(12), 1950-1953[Stand Hot and Cold Normal Water Dispenser with Refrigerator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ACP Aluminium Cmposite Panel Cladding ExtrusionsAuthor for correspondence