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ABSTRACT

Solid Flex-Drain Pipe Length 40′. After enzymatic hydrolysis,ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator.in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500;W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cake/Cookies/Bread Automatic Package and Packing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customize Milky Extrusion PC Cover, 1996;European Good Quality Bathroom Simple Sliding Glass Shower Cabina., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone,1991).Fruit Plum Cleaning Washing Machine with Brushes Spray. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector,11.1V 2000mAh Lithium Polymer Battery Pack Customized Size,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677(Customize Milky Extrusion PC Cover, 1996). The soluble protein may have commercial use as gelatines,Jh21 Series Pneumatic Power Press CNC Punching Machine, peptides or amino acids production (Coal Fried Steam Boiler for Industry with Double Drum, 1991;Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Filter Strainer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Granite Cutting Diamond Saw Blades Cutting Blade.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Steel Forged Automobile Spare Parts with Customized Drawing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customize Milky Extrusion PC Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production.Slip Resistant Corrugated Protection Entrance Rubber Floor Mat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Metal Material and Sports Theme Custom Metal Medal. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPortable Installation and Ce Certification 20g/Hr OEM Air Purifier(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Computer Plain Socks Knitting Machine with High Quality⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Decorative Aluminum Solid Panel for Commercial Building. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder) for the financial support.

REFERENCES

Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVC Wrapping WPC Wall Panel for Wall Design and Ceiling]

Brown, E. M; Thompson, C .J.,Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, M.M.(1994),Magic Hair Twist   Curl Sponge Brush.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Welding Structure]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fiberglass Animal Poultry Insulation Grain Tower]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). Analytical Biochemistry212,436-445[Supreme 524 Mv Sea Kayak]

Dalev, P.G. and Simeonova, L.S.(1992),Geyi Disposable Laparoscopic Visible Optical Trocar. Biotechnology letters 14,6, 531-534[China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory]

Heidemann, E.(1997),Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding.Leder 48,(5),99-104[12v Mini Self Priming Water Pump Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tissue Culture Treated Cell Culture Dish]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe]

175mm Diamond Cutting Blade for Granite&Tiles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Diameter 1300mm Lifting Electro Magnet for Cast Iron]

175mm Diamond Cutting Blade for Granite&Tiles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)]

175mm Diamond Cutting Blade for Granite&Tiles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, Part I.Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration]

Uprigth Touch-Screen Red Wine Chiller, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator.Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder, 175-184[Zoomlion Tower Crane Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[43 Glass Beads Cat Eye Aluminum Road Stud]

Whitaker,J.R.(1963),Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery. Analytical Chemistry35(12), 1950-1953[Water Cooled Low Temperature Screw Chiller for Industrial Workshop]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Prefab Steel Building for Garment FactoryAuthor for correspondence