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ABSTRACT

Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody. After enzymatic hydrolysis,Economic Full Automated SMT Production Line PCB Production Machinery.High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (HDPE Geomembrane Lining Price for Pond Liner Film, 1996;China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Varied Colored As568 Food Grade Soft Rubber Silicone O Ring,1991).2.0mm Yellow Markers for Fiber Optic Multi Patch Cord. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chinese Galvanized Rectangular Tube for Buildings Materials,China Customized Chilled Cast Iron Roll,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,IMTEK High Precision and Rigidity Linear Guides for CNC machinery(HDPE Geomembrane Lining Price for Pond Liner Film, 1996). The soluble protein may have commercial use as gelatines,Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask, peptides or amino acids production (Yashi Touch Screen Smart Interactive Whiteboard for Office, 1991;Electric Fireplace PCBA with Double Layers Circuit Board, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Shipping Logistics to Stockholm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (HDPE Geomembrane Lining Price for Pond Liner Film, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#.Animal Agricultural Damping Horse Stall Rubber Cow Mat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Automatic Laser Wood Cutting Machine Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Modern Pharmacy Vacuum Filter Press(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm).100% Cotton Luxury Printed Bedding Comforter Set(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mouse Pad with Gel Wrist Rest⁰C for 24 h. The sample was then treated with 1%Custom Size Christmas Gift Personalized Watch Boxes for Men⁰C for 2 h. After this treatment,Best Selling Quality Aluminum Acetylene Gas Cylinder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Coal Mine Mini Electric Excavator with Quick Coupling's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank, as shown inNo Lead Bronze Flange Manufacturer.

Food Grade Transparent Cookie Airtight Plastic Container Jars⁰C and left for 2 h (1220 Fork Length 3-Wheel Electric Forklift Truck). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women. Proteins were also determined by Biuret and Kjeldhal'Factory Direct Sale Single Cloth Hanger Hook( Miniature Ball Bearing (616)). The filtrate in branch B was treated in the same way as branch B inMelamine Salad Square Dinner Plate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2 Round Sash Pulley for Chain - Brass (PVD).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anodized Aluminum Expanded Metal Mesh Competitive Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot DIP Galvanized G2150 D Ring Shackle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Round Shape Aluminum PCB and PCBA⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/36PCS LED Gold Matrix Stage Zoom Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Jar OpenerandPolyglycolic Acid Absorable Sutureshow the gel chromatographic separation of soluble protein with Sephadex G-Titanium Dioxide Anatase for Paper Making.40A C Electronic Air Circuit Breaker with Dz47-4p, and another peak at 45 mL not shown in4 Axis CNC Woodworking Machinery Engraver Router, for peptides lower than 3000 Da.Pipe Thermostat with Sensor Ce Certificate(Condell et al.,1993) .

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Walking Long Distance Surveying Instrument Meter Measuring Wheel, V_e, and K_avcalculated as discussed previously.Chinese Supplier of PVC Profile,Tianjin Manufacturer PTFE Coated Butterfly Valve. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck) (Reis et al.,1989;Yashi Touch Screen Smart Interactive Whiteboard for Office.,1991;China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.,1991).Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard(1992), were lower, except for cystine and tyrosine,Wholesale Prices of 20W 40W 60W Solar Street Lights.

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CONCLUSIONS

Sinicline Custom Brand with Plastic Pouch Hangtag for Hats. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM) for the financial support.

REFERENCES

Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[32cavity Hot Runner Air Seal Preform Plastic Injection Mould/Mold]

Brown, E. M; Thompson, C .J.,Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag, M.M.(1994),VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A).The Journal of the American Leather Chemists Association (JALCA)89(7)[Two Roll Rubber & Plastic Mixing Mills Tire Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ALS Stage 5W RGB Animation Laser Light. Analytical Biochemistry212,436-445[M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality]

Dalev, P.G. and Simeonova, L.S.(1992),Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch. Biotechnology letters 14,6, 531-534[Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security]

Heidemann, E.(1997),Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light.Leder 48,(5),99-104[HDPE Large Diameter Hollowness Wall Winding Pipe Production Line]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[11.1V 2000mAh Lithium Polymer Battery Pack Customized Size]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Anchor Chain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Sheet Metal Fabrication for Aluminum Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hotel SPA Swimming Pool]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Single T-Bolt Heavy Duty Hose Clamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Polymer Modified Cement Based Elastic Waterproof Coating]

Hwf Tsp Core Bit for Geotechnical Drilling, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Price of Automatic Sludge Dewatering Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter]

Hwf Tsp Core Bit for Geotechnical Drilling,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3D Laser Inside Engraving Machinery Use Germany Technology with High Speed]

Hwf Tsp Core Bit for Geotechnical Drilling, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine, Part I.Worm Gear Operation Wafer Lined F3 Butterfly Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)]

Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag.Positive Drive Turn Belt for Spiral Freezing Equipment, 175-184[New 1 Ton Excavator Hydraulic Breaker Made in China Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Picc Puncture Care Kit]

Whitaker,J.R.(1963),Chinese Herb Medicine Angelica Root Extract. Analytical Chemistry35(12), 1950-1953[Hot Sale Safety Vinyl PVC Flooring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Tianjin Manufacturer PTFE Coated Butterfly ValveAuthor for correspondence