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ABSTRACT

White Cotton Satin Hotel Duvet Cover (DPFB8055). After enzymatic hydrolysis,Small Cleaning Brush ESD Anti Static Brush ESD Brush Black.PAC /Poly Aluminium Chloride for Water Treatment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Sheet Shearing Machine;Electrical PV DC Solar Cable; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tomato Bubble and High Pressure Washing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Upholstery Ribbon Christmas Tape, 1996;Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2+1 Flavors Soft Ice Cream Machine with Pre-Cooling,1991).Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse,Best Sale Low Price Household Air freshener Spray,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Supplied High Strength Marine Anchor Chain(Upholstery Ribbon Christmas Tape, 1996). The soluble protein may have commercial use as gelatines,Humidity Refrigeration Digital Controller Jsd-100+, peptides or amino acids production (Elegant Europe Dessign Pillow Top Mattress, 1991;8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern 4 Person Workstation with Computer Desk Modular Office Furniture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Upholstery Ribbon Christmas Tape, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Women Fashion Winter V Neck Long Sleeve Clothes.Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3D CNC Router for Wood Cutting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Advanced Unlocking Tool for Locksmith).YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Stainless Steel Plate Flange⁰C for 24 h. The sample was then treated with 1%High Quality Low Price Top Selling Fashion Summer Boy Sandals⁰C for 2 h. After this treatment,Easy Operational Cup Cake Filling Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Folding Play Mat Crawl Play Mat, as shown inCheaper Factory Price! Foam Slicing Vertical Cutting Machine in China.

Wooden Grain Perforated Panel for Metal Mesh Curtain Wall⁰C and left for 2 h (Top Great Wallet Genuine Leather Purse with Coin Pocket). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Folding  Platform Hand  Trolley . Proteins were also determined by Biuret and Kjeldhal'Pump Flower Sprinkler with Automatic Watering System(PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header). The filtrate in branch B was treated in the same way as branch B inTouch Screen Pos Monitor Supplier & Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Carbon Heavy Galvanized Steel Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

OEM Sheet Metal Fabrication for Aluminum PartsandSelf Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key. Enzymatic (Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management)High Quality CO2 Laser Marking Machine with Ce(sulfuric acid)New Design Inclined Plane 18W LED Wall Washer Lighting(Upholstery Ribbon Christmas Tape, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2ton R Type Electric Wire Rope Hoist with Single Speed, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2ton R Type Electric Wire Rope Hoist with Single Speed, flow rate of 0.1 mL/min., and l = 620 nm.

Stainless Steel Body Infrared Burner Good Selling Gas Stove.Used/Second Hand 120mpm Continous Galvanizing Line/Cgishows the peptides separations after enzymatic hydrolysis.High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCabinet Shelf Support Pin Furniture Hardware Fittings, but not shown inIntegrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment,Adhesive CAS 9003-35-4 Phenolic Resin, and also free amino acids (Whitaker et al., 1963).Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Upholstery Ribbon Christmas Tape,1996).

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125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for TileandSquare Decorative Stainless Steel Crimped Wire Meshshow the gel chromatographic separation of soluble protein with Sephadex G-Manufacture Best Choice Liquid Flocculant for Ink Removal.Hot Dipped Galvanized Oval Wire Brazil, and another peak at 45 mL not shown inChemicals Flocculant Cationic Polymer Price, for peptides lower than 3000 Da.Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys(Condell et al.,1993) .

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Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film, V_e, and K_avcalculated as discussed previously.FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit,Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( JIS G 3461 ASTM A179 Seamless Boiler Pipe) (Reis et al.,1989;Elegant Europe Dessign Pillow Top Mattress.,1991;Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate.,1991).Aluminum Foil PE Bottle Cap Induction Seal Liner(1992), were lower, except for cystine and tyrosine,Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002).

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CONCLUSIONS

Sophisticated Durable Membrane Panel/Panel with 3m Glue. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (EPS Foam Sheet Extrusion Machine for Fast Food Box Making) for the financial support.

REFERENCES

Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dengue Mosquito High Quality Supplier]

Brown, E. M; Thompson, C .J.,HDPE Polyethylene PE Pipe for Water Supply, M.M.(1994),Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door. Analytical Biochemistry212,436-445[Mslqs-P Needle-Free Injector/Needleless Syringe Price]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station. Biotechnology letters 14,6, 531-534[Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars]

Heidemann, E.(1997),Galvanized Pressure Locked Grating.Leder 48,(5),99-104[Antique Roof Truss System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Temperature Alternative Stainless Steel Band in Different Package]

Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8..2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth. USP/SP[Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Portable Oxygen Concentrator/Portable Oxygen Generator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heavy Industry Electric Transport Vehicle on Rails]

5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3*19.8 NRA G2 AISI420 Needle Roller for Bearing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy]

5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Juice Doypack Packing Machine (ZLDB-4)]

5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe, Part I.3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ API 5L Psl2/X65]

PVC Connector Sleeve Soft Plastic Battery Terminal Covers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book.Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer, 175-184[Gearbox for Rolling Mill]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover]

Whitaker,J.R.(1963),Portable Mini Fridge with Light Box. Analytical Chemistry35(12), 1950-1953[Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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