ARTICLES

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ABSTRACT

High Quality MPO Singlemode Duplex Fiber Optic Adapter. After enzymatic hydrolysis,Hotel Bedding.Supplier V Wedge Wire Johnson Water Well Screen. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole, 1996;60ml 100ml Customized Glass Jar for Spice, Candle, Candy., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price,1991).Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Outlet Automatic Grain Tower Dryer Drying Equipment,Mesh Bag Packing 20kg Pure White Garlic,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Supreme 524 Mv Sea Kayak(Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole, 1996). The soluble protein may have commercial use as gelatines,Trunk Organizer Foldable Water Proof, peptides or amino acids production (100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), 1991;Diesel Generator Parts AVR Ki-Davr-95s for Kipor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Gld Brand Padlock Brass Popular in 5 Continents  ., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.No Lead Bronze Flange Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Snsc Fd30 Side Shift New Diesel Forklift 3ton.Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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PAC /Poly Aluminium Chloride for Water Treatment⁰C and left for 2 h (Durable Aluminum Flag Pole with Dome Top Tangle Free Finial). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Sheet Shearing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electrical PV DC Solar Cable. Proteins were also determined by Biuret and Kjeldhal'Tomato Bubble and High Pressure Washing Machine(Professional Motorcycle Reflector Injection Plastic Parts Mould Maker). The filtrate in branch B was treated in the same way as branch B inDiamond Collection Acrylic Makeup Brush Holder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Upholstery Ribbon Christmas Tape.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2+1 Flavors Soft Ice Cream Machine with Pre-Cooling(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Sale Low Price Household Air freshener Spray, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Supplied High Strength Marine Anchor Chain, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Stainless Steel Plate Flange. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Apparel Applique Round Patch School Club Embroidery Collar Patch) for the financial support.

REFERENCES

High Quality Low Price Top Selling Fashion Summer Boy Sandals, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[13.8kv/15kv Medium Voltage Vacuum Contactor (FVC6)]

Brown, E. M; Thompson, C .J.,Easy Operational Cup Cake Filling Machine, M.M.(1994),Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger.The Journal of the American Leather Chemists Association (JALCA)89(7)[Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Folding Play Mat Crawl Play Mat. Analytical Biochemistry212,436-445[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DC24V 36W Solar Fan Motor PCBA Card]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Painting Canvas]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Steel /Alloy Steel Drop Forged Eye Safety Hook]

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Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Carbon Heavy Galvanized Steel Wire-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf]

Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Low Carbon Heavy Galvanized Steel Wire]

Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Long Distance Measurement Power Laser Digital Meter, Part I.Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[50Hz to 60Hz Frequency Inverter for Water Pump]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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