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ABSTRACT

Single Screw Extruder for Plastic PP/PE Pipe Production Line. After enzymatic hydrolysis,Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck.Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Commercial Use Click Lock Standard Spc Vinyl Plank Flooring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, 1996;High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Precision Customized OEM Auto Hardware Lathe CNC Machining Parts,1991).White Color Enamel Bowls with High Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door,Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board(Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, 1996). The soluble protein may have commercial use as gelatines,Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration, peptides or amino acids production (Southeast Asia Healthy Soft Integrated Latex Foam Mattress, 1991;12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Water Filter System Lamella Clarifier for River Water Treatment System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cabinet Shelf Support Pin Furniture Hardware Fittings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Reasonable Price High Quality Nylon Cable Gland.Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Disposable Anesthesia Breathing Circuit Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Speed Cable Wire Winding Braiding Machine).Road Safety Warning Barricade LED Solar Powered Blinking Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manufacturer Customized Plastic Injection Molding⁰C for 24 h. The sample was then treated with 1%Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)⁰C for 2 h. After this treatment,Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light, as shown inCustom Size Christmas Gift Personalized Watch Boxes for Men.

Solar Energy Storad Power Generation System⁰C and left for 2 h (IMTEK High Precision and Rigidity Linear Guides for CNC machinery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Aluminum Cover Frame Half Aluminum Cover Profile. Proteins were also determined by Biuret and Kjeldhal'Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility(18V Lithium Battery Powered Cordless Drill). The filtrate in branch B was treated in the same way as branch B inBlack Color Coating High Density Fiber Cement Board 8mm Outdoor and Indoor Wall Panel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DC24V 36W Solar Fan Motor PCBA Card.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Monocular Hunting Wargame Mini Scope Digital Night Vision. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Italy Design Good Quality Hydraulic Motorcycle Lift Table⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Lab Equipment Sr Paper Pulp Beating Freeness Test Machine.Forged Steel Pressure Sealed Bonnet Globe Valveshows the peptides separations after enzymatic hydrolysis.CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness, but not shown inHigh Power From Han′s GS 2000W Laser Cutter,Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima, and also free amino acids (Whitaker et al., 1963).Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop,1996).

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CONCLUSIONS

OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126) for the financial support.

REFERENCES

Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Machinery Spare Parts Strengthened Ladder for Tower Crane]

Brown, E. M; Thompson, C .J.,Galvanized Eye Grab Bend Hook, M.M.(1994),Transparent LED Display P3.91 Glass Wall LED Screen.The Journal of the American Leather Chemists Association (JALCA)89(7)[SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Surgical Power Tool Medical Care Orthopedic Mini Bone Saw. Analytical Biochemistry212,436-445[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Dalev, P.G. and Simeonova, L.S.(1992),Micro Small Plastic Product Injection Molding Making Machine. Biotechnology letters 14,6, 531-534[Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding]

Heidemann, E.(1997),Glass Lifting Machine with Sucker for Glass Movements.Leder 48,(5),99-104[Acrylic or Plastic Material Transparent Business Cards (HW-802)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fashion Shoulder Backpack Handbag School Bag]

Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Anodized Gold Brush Finish Aluminum Sheet Coil.Popular Industrial Sewing Quilting Machine With CO. USP/SP[Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Security Shopping Trolley Baby Seat for Supermarket Cart]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Silver Anodized Aluminium I Beam Level Measuring Tool]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Book Tissue Box Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh]

Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pure Sine Wave Power Frequency Inverter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rechargeable Battery Operated Cooling Folding Electric Fan]

Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop]

Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, Part I.Colorful 100% Cotton Hotel Face Towels. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly]

High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),33kv Dry Type Resin Casted Distribution Transformer.Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27, 175-184[Cycjet UV Laser Marking Machine for Carton and Plastic Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sw Type BOPP Packaging Adhesive Tape Coating Machine]

Whitaker,J.R.(1963),Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate. Analytical Chemistry35(12), 1950-1953[Upholstery Ribbon Christmas Tape]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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