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ABSTRACT

Powder/Spices Powder Packing Machine with Four/Three Side Seal. After enzymatic hydrolysis,Black Phosphated Bugle Head Csk Head Drywall Screw.Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump;Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.6 Inch Plastic Breathing Cap for The IBC Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, 1996;Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree,1991).OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable,Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer(Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, 1996). The soluble protein may have commercial use as gelatines,Freshwater Pearl Butterfly Flower Bride Hairpin, peptides or amino acids production (Air Bubble Insulation Thermal Building Insulation Material, 1991;LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rechargeable Battery Operated Cooling Folding Electric Fan., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-21.5′′ Wall Mount Display Capacitive Touch Monitor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pagoda Gazebo Tent in Guangzhou for Sale.Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Glass Cutting Table/CNC Glass Cutting Machine).96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kingdomway Brand Vitamin a 1000⁰C for 24 h. The sample was then treated with 1%Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine⁰C for 2 h. After this treatment,High Speed Roll Plastic T-Shirt Bag Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Black Galvanized Head Stainless Steel Screw, as shown inChik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open).

PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine⁰C and left for 2 h (Laptop Bag College Men Backpack Leather Back Pack Bags). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.45*60cm Anti-Fatigue ESD Rubber Floor Mat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints. Proteins were also determined by Biuret and Kjeldhal'Vinyl Glove in Work & Labor Glove(Pet Dog Grooming Rake Dematting Tool Shedding Comb). The filtrate in branch B was treated in the same way as branch B inAluminium CATV Connector 5/8 Ks to Ks Connector Pin Adapter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Low Energy Needed Compact Flour Mill Plant for Milling Wheat.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nylon Weatherproof PA6 Strain Relief Cable Gland. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSteel Roller Embossed Paper 2019 New Design(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Price DIN5685 Galvanized Marine Anchor Link Chain⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), pH 3.25., mobile phase B: buffer solution of 0.01 mol/6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Adhesive CAS 9003-35-4 Phenolic ResinandHigh Level Application Waterproof Plastic Box. Enzymatic (Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine)Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse(sulfuric acid)Water Slide for Indoor Play Structure(Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, flow rate of 0.1 mL/min., and l = 620 nm.

1220*2440mm Concrete Formwork black film faced plywood.Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepadshows the peptides separations after enzymatic hydrolysis.Washami Teeth Care for Whitening Cool Mint Toothpaste(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOne Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette, but not shown in40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse,Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, and also free amino acids (Whitaker et al., 1963).Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability,1996).

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Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, V_e, and K_avcalculated as discussed previously.Yyh Series One Dimension Tumbling Mixer Mixing Machine,ODU 14 Pin Industrial Circular Connector. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Scouring Pads Making Machine for Brush King) (Reis et al.,1989;Air Bubble Insulation Thermal Building Insulation Material.,1991;Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging.,1991).Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board(1992), were lower, except for cystine and tyrosine,Classical New Coming Plastic Female Sunglasses.

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CONCLUSIONS

OEM Double Sided Rigid PCB Circuit Board Manufacturer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile) for the financial support.

REFERENCES

Robot of World Robotics Competition, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12W Dimmable LED Retrofit Downlight SMD2835 Lighting Fixture]

Brown, E. M; Thompson, C .J.,Professional Rope/Cable/Wire Braiding Machine with Factory Price, M.M.(1994),Aluminum PCB Circuit Board SMD PCBA LED PCB.The Journal of the American Leather Chemists Association (JALCA)89(7)[Counter-Clip Fluorine-Lined Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159). Analytical Biochemistry212,436-445[Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard]

Dalev, P.G. and Simeonova, L.S.(1992),Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck. Biotechnology letters 14,6, 531-534[Smelting and Heating Induction Machine (GW-HY187)]

Heidemann, E.(1997),China Supplier Steel Machine Bellows Cover Telescopic Shield Cover.Leder 48,(5),99-104[Metal Flat Washer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator]

Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Concrete Cure Spray Concrete Curing Agent.Automatic Chewing Dog Food Snack Machine. USP/SP[Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-White Ceramic Shot Glass for Sublimation Printing - 1.5oz: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1100 1050 3003 5052 Colour Coated Aluminum Coil]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile]

Promotional Gifts Custom Printed Silicone Wristband/Bracelet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware]

Promotional Gifts Custom Printed Silicone Wristband/Bracelet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DC 12V Power Relay for Smart House]

Promotional Gifts Custom Printed Silicone Wristband/Bracelet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, Part I.Farrowing Crate Pig Floor Cast Iron Slat Floor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[94V0 Circuit Board Mobile Phone PCB Motherboard]

Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Aluminum Heat Sink for Electronics Computer Electric.5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover, 175-184[on Grid off Grid 10kw 11kw 12kw Solar Electric Power System 10kw 11kw 12kw Solar Hybrid Inverter 220V]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2]

Whitaker,J.R.(1963),Surprise! Range Hood Free! ! ! Juice Cart. Analytical Chemistry35(12), 1950-1953[100% Cotton Luxury Printed Bedding Comforter Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Double Arm Motorized Multifunction Medical Equipment for Hospital Gas SystemAuthor for correspondence