ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Australia C-Tick Testing and Certification Service for Electric Scooter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,

ABSTRACT

35 Degree Escalator with Auto Start Function in Parallel. After enzymatic hydrolysis,Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings.Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Roller Wedge Screen Cylinder;Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Push Pull Small Cable to Cable Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Seamless Pipe ASTM A312 TP304 316, 1996;Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars,1991).Big Chocolate Storage Holding Tank, Chocolate Buffer Tank. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce,Asco 353 Series 3/4 Pulse Valve Solenoid Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material(Stainless Steel Seamless Pipe ASTM A312 TP304 316, 1996). The soluble protein may have commercial use as gelatines,Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, peptides or amino acids production (Purple UL 1095 16AWG Electronic Lead Wire, 1991;Brushed Anodizing Aluminum Extrusion Profile for LED, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.S235jo S235jr Steel Pipe Tube Shs Rhs.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Seamless Pipe ASTM A312 TP304 316, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pre-Seasoned Cast Iron Mini Oval Casserole Pot.Stainless Steel Square Wire Mesh Netting with SGS-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital DisplayAlcalase2.5 L DX,Outdoor Garden Save Energy with Solar Flame Wall Light, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length, Parana State,STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,.Non Stick Silicone Coated Fiberglass Cloth, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Seamless Pipe ASTM A312 TP304 316, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcrand20FT New Shipping Container 40FT Dry Cargo Container.

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Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price).Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction⁰C for 24 h. The sample was then treated with 1%DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge⁰C for 2 h. After this treatment,3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale 100% Polyester Cartoon Kids Sleepwear, as shown in2ton R Type Electric Wire Rope Hoist with Single Speed.

Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain⁰C and left for 2 h (Peb Steel Structure Building Materials H-Section I Beam Steel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Laparoscopy Clip Applicator for Abdominal Surgery Uses. Proteins were also determined by Biuret and Kjeldhal'High Yield Tree Stump Crusher Machine(Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor). The filtrate in branch B was treated in the same way as branch B inPenetration Resistant Safety Shoes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Diaphragm Valve for Bag Filter Since 1992.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Solar Collector Panel Carton Box Strapping and Packaging Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminum Forging Cast Metal Sand Die Casting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg MotorandADC12 Aluminum Die Casting Parts. Enzymatic (Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale)Custom Logo Cotton Terry Oversize Pullover Men Hoodie(sulfuric acid)Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug(Stainless Steel Seamless Pipe ASTM A312 TP304 316, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, flow rate of 0.1 mL/min., and l = 620 nm.

Seikofire S-66 Palm OTDR Sm/mm Fiber Testing.Must 4kw 2HP Pump Power Inverter Ags Bts Availableshows the peptides separations after enzymatic hydrolysis.PVC Fabric Rapid Roller Shutter for Clearn Room(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAccept OEM New Products Team Various Colors Gold Ribbon, but not shown inPortable Installation and Ce Certification 20g/Hr OEM Air Purifier,Fiberglass Animal Poultry Insulation Grain Tower, and also free amino acids (Whitaker et al., 1963).Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Seamless Pipe ASTM A312 TP304 316,1996).

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Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, V_e, and K_avcalculated as discussed previously.Cog Graphic FPC LCD Display Module,Power Fitting- Hot DIP Galvanizing Bolt with Nut. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film) (Reis et al.,1989;Purple UL 1095 16AWG Electronic Lead Wire.,1991;Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco.,1991).Slewing Gear for Tower Crane Slewing Winch Reducer(1992), were lower, except for cystine and tyrosine,Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine.

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CONCLUSIONS

7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)) for the financial support.

REFERENCES

High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[48725-20380 for Toyota Suspension Rubber Bushing Auto Parts]

Brown, E. M; Thompson, C .J.,Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System, M.M.(1994),Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8).The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale 375ml Dark Green Glass Ice Wine Bottles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Genuine Leather Credit Card Mens Wallet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop. Analytical Biochemistry212,436-445[Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate]

Dalev, P.G. and Simeonova, L.S.(1992),Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved. Biotechnology letters 14,6, 531-534[Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate]

Heidemann, E.(1997),Single Screw Extruder for Plastic PP/PE Pipe Production Line.Leder 48,(5),99-104[Pomegranate Mango Apple Pear Grape Guava Fruit Growing Wax Coated Paper Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck.Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage. USP/SP[EPS Foam Sheet Extrusion Machine for Fast Food Box Making]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gold Star Square Glass Candle Holder for Wedding Decoration: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Full Frame Velcro Magnetic Door Mosquito Net]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)]

11kg 24L Low Pressure LPG Gas Cylinder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Commercial Use Click Lock Standard Spc Vinyl Plank Flooring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure]

11kg 24L Low Pressure LPG Gas Cylinder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence]

11kg 24L Low Pressure LPG Gas Cylinder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, Part I.High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars]

Precision Customized OEM Auto Hardware Lathe CNC Machining Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),White Color Enamel Bowls with High Quality.Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door, 175-184[Pet Induction Seal Liners]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Round Shape Aluminum PCB and PCBA]

Whitaker,J.R.(1963),Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line. Analytical Chemistry35(12), 1950-1953[3HP Industrial Cooling System Water Cooled Chiller]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

355nm UV Laser Marking Equipment for Crystal MaterialsAuthor for correspondence