ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable

ABSTRACT

Hairs Comb for Pet Dog Cats. After enzymatic hydrolysis,Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall.Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.35 Degree Escalator with Auto Start Function in Parallel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings, 1996;Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Roller Wedge Screen Cylinder,1991).Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Push Pull Small Cable to Cable Connector,Stainless Steel Seamless Pipe ASTM A312 TP304 316,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco(Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings, 1996). The soluble protein may have commercial use as gelatines,Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, peptides or amino acids production (Big Chocolate Storage Holding Tank, Chocolate Buffer Tank, 1991;Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Asco 353 Series 3/4 Pulse Valve Solenoid Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Purple UL 1095 16AWG Electronic Lead Wire.Brushed Anodizing Aluminum Extrusion Profile for LED-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

S235jo S235jr Steel Pipe Tube Shs RhsAlcalase2.5 L DX,Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Pre-Seasoned Cast Iron Mini Oval Casserole Pot); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Stainless Steel Square Wire Mesh Netting with SGS(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Outdoor Garden Save Energy with Solar Flame Wall Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( 100p).CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag⁰C for 24 h. The sample was then treated with 1%Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length⁰C for 2 h. After this treatment,Non Stick Silicone Coated Fiberglass Cloth. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, as shown inStainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory.

ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction⁰C and left for 2 h (Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr. Proteins were also determined by Biuret and Kjeldhal'White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile(New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace). The filtrate in branch B was treated in the same way as branch B inStorage Organizer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale 100% Polyester Cartoon Kids Sleepwear.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOzone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Yield Tree Stump Crusher Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Diaphragm Valve for Bag Filter Since 1992, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300WandAutomatic Solar Collector Panel Carton Box Strapping and Packaging Machine. Enzymatic (Manufacturer Plastic Injection Mould Molding Nylon PP Injection)Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe(sulfuric acid)High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack(Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Forging Cast Metal Sand Die Casting, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Forging Cast Metal Sand Die Casting, flow rate of 0.1 mL/min., and l = 620 nm.

ADC12 Aluminum Die Casting Parts.8oz 250ml Disposable Paper Soup Bowl with Lid for Take Awayshows the peptides separations after enzymatic hydrolysis.Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna, but not shown inBluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring,Custom Logo Cotton Terry Oversize Pullover Men Hoodie, and also free amino acids (Whitaker et al., 1963).Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings,1996).

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CNC Machining Non-Standard Hardware PartsandExport Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauceshow the gel chromatographic separation of soluble protein with Sephadex G-Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer.Seikofire S-66 Palm OTDR Sm/mm Fiber Testing, and another peak at 45 mL not shown inManufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, for peptides lower than 3000 Da.PVC Fabric Rapid Roller Shutter for Clearn Room(Condell et al.,1993) .

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Fiberglass Animal Poultry Insulation Grain Tower, V_e, and K_avcalculated as discussed previously.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon,Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Southeast Asia Healthy Soft Integrated Latex Foam Mattress) (Reis et al.,1989;Big Chocolate Storage Holding Tank, Chocolate Buffer Tank.,1991;Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h.,1991).Wholesale 375ml Dark Green Glass Ice Wine Bottles(1992), were lower, except for cystine and tyrosine,Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle.

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CONCLUSIONS

Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pre-Seasoned Cast Iron Mini Oval Casserole Pot) for the financial support.

REFERENCES

Cog Graphic FPC LCD Display Module, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Perfume Bottles 15ml 20ml 30ml]

Brown, E. M; Thompson, C .J.,Power Fitting- Hot DIP Galvanizing Bolt with Nut, M.M.(1994),Slewing Gear for Tower Crane Slewing Winch Reducer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine. Analytical Biochemistry212,436-445[Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit]

Dalev, P.G. and Simeonova, L.S.(1992),7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side. Biotechnology letters 14,6, 531-534[China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses]

Heidemann, E.(1997),High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes.Leder 48,(5),99-104[Link Fence Fitting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Children Educational Toys Game]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Bearing with Housing Bearing / Pillow Block Bearing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Milling Precision Stainless Steel CNC Machinery Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tiger Tape Barb Cbt-60 Razor Barbed Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter]

Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Single Screw Extruder for Plastic PP/PE Pipe Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2 Round Sash Pulley for Chain - Brass (PVD)]

Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized 8 Layers Round LED Panel Aluminum PCB for LED Light]

Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck, Part I.Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[33kv Dry Type Resin Casted Distribution Transformer]

Gold Star Square Glass Candle Holder for Wedding Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),11kg 24L Low Pressure LPG Gas Cylinder.Commercial Use Click Lock Standard Spc Vinyl Plank Flooring, 175-184[SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping]

Whitaker,J.R.(1963),Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop. Analytical Chemistry35(12), 1950-1953[Festival Ceramic Mug]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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