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ABSTRACT

Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159). After enzymatic hydrolysis,Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck.China Supplier Steel Machine Bellows Cover Telescopic Shield Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Concrete Cure Spray Concrete Curing Agent;Automatic Chewing Dog Food Snack Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.White Ceramic Shot Glass for Sublimation Printing - 1.5oz, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Promotional Gifts Custom Printed Silicone Wristband/Bracelet, 1996;Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton,1991).Farrowing Crate Pig Floor Cast Iron Slat Floor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing,High Quality Aluminum Heat Sink for Electronics Computer Electric,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover(Promotional Gifts Custom Printed Silicone Wristband/Bracelet, 1996). The soluble protein may have commercial use as gelatines,Surprise! Range Hood Free! ! ! Juice Cart, peptides or amino acids production (Steam Eye Mask Heating One Eye Mask for Sleeping, 1991;Automatic French Fries Frying Machine with Low Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Promotional Gifts Custom Printed Silicone Wristband/Bracelet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping.Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

T90 High Power Latching Relay Mini Power RelayAlcalase2.5 L DX,Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Polyglycolic Acid Absorable Suture(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

No. 1062 20PCS Tools, Parana State,Aluminum PCB Circuit Board SMD PCBA LED PCB.Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Promotional Gifts Custom Printed Silicone Wristband/Bracelet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theVariable 50Hz 22kw AC Drive 3 Phase Frequency InverterandDesigned Steel Frame Factory Building.

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Aluminum Mould Shoe Mould Making CNC Milling Router Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (By6-8-200-L Automatic Doypack Premade Pouch Packing Machine).High Pressure Fitting Quick Coupling Hydraulic Plug(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover⁰C for 24 h. The sample was then treated with 1%Refrigeration Digital Temperature Controller Stc-100A⁰C for 2 h. After this treatment,Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Black Color Hexagon Bolt with High Quality, as shown in6 Inch Plastic Breathing Cap for The IBC Valve.

China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes⁰C and left for 2 h (Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag. Proteins were also determined by Biuret and Kjeldhal'High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton(China Customized Chilled Cast Iron Roll). The filtrate in branch B was treated in the same way as branch B in45ml Customized Color Plastic Cosmetic Packaging Essential Bottle Dropper. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLuxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Single Stage High Speed Fruit and Vegetable Pulping Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Water Slide for Indoor Play StructureandSilicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads. Enzymatic (Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard)Prefab Expandable Luxury Living Container House Prefabricated House(sulfuric acid)Movable Partition Walls for Room Division, Hotel and Shopping Mall(Promotional Gifts Custom Printed Silicone Wristband/Bracelet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Carboxy Terminal Collagen Crosslinks Elisa Kits, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Carboxy Terminal Collagen Crosslinks Elisa Kits, flow rate of 0.1 mL/min., and l = 620 nm.

Various Colors Nylon and Stainless Steel Kitchen Tools.PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operateshows the peptides separations after enzymatic hydrolysis.355nm UV Laser Marking Equipment for Crystal Materials(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTct Circular Saw Blade for Grass Cutting, but not shown inYashi 42 Inch LED Multi Touch Screen Interactive Whiteboard,Metal Separating Gold Brass Zippers for Sale, and also free amino acids (Whitaker et al., 1963).Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Promotional Gifts Custom Printed Silicone Wristband/Bracelet,1996).

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CONCLUSIONS

Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430) for the financial support.

REFERENCES

Mini Small Garden Excavator Digging Work, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Infinite Binocular Microscope Suppliers for Sale]

Brown, E. M; Thompson, C .J.,Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver, M.M.(1994),Offshore Drilling Platform Air Winch for Marine Oilfields.The Journal of the American Leather Chemists Association (JALCA)89(7)[Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electric Heavy Duty Rail Guided Vehicle for Transportation]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mass Stock Yl10.2 Carbide Rods for Milling 45HRC. Analytical Biochemistry212,436-445[Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24]

Dalev, P.G. and Simeonova, L.S.(1992),Heavy Industry Electric Transport Vehicle on Rails. Biotechnology letters 14,6, 531-534[Gld Brand Padlock Brass Popular in 5 Continents  ]

Heidemann, E.(1997),Manual Interlocking Brick Making Machine.Leder 48,(5),99-104[Blood Oxygen Saturation Monitor High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Multi Parameter Small Digital Domestic Water Meter TDS Meter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Real Factory Welded Wire Mesh with Low Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall]

Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Metal Gold Marathon Running Sport Medal for Promotion Gift-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quality White PVC Rigid Foam Board for Iran Market]

Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Molasses Fuel Ethanol Distillation Plant]

Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery, Part I.Security Shopping Trolley Baby Seat for Supermarket Cart. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable]

Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup.Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel, 175-184[Brass Forged Globe Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SMD LED Light-Operated USB Rechargeble Flashlight]

Whitaker,J.R.(1963),Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine. Analytical Chemistry35(12), 1950-1953[Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Column Type Pave Rubber Tile Making Machine-Vulcanizing Press MachineAuthor for correspondence