ARTICLES

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ABSTRACT

A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve. After enzymatic hydrolysis,Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing.Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Security Shopping Trolley Baby Seat for Supermarket Cart, 1996;Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup,1991).Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine,Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manual Foot Pedal Hydraulic Pump(Security Shopping Trolley Baby Seat for Supermarket Cart, 1996). The soluble protein may have commercial use as gelatines,860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag, peptides or amino acids production (Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women, 1991;CH Series Pid Intelligent Temperature Controller CH402, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Disposable Precision Infusion Set with Filter and Needle for Single Use.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Low Price Sweep Frequency Response Analyzer for Power Transformer Test. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Security Shopping Trolley Baby Seat for Supermarket Cart, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500.Stainless Steel Mechanic Parts for Lock Accessories with MIM Process-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, Parana State,Manual Interlocking Brick Making Machine.High Quality MPO Singlemode Duplex Fiber Optic Adapter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Security Shopping Trolley Baby Seat for Supermarket Cart, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance PartsandWet Type Wire Drawing Machine for Carbon Steel and Stainless Steel.

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Hotel Bedding(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine).Supplier V Wedge Wire Johnson Water Well Screen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles⁰C for 24 h. The sample was then treated with 1%10A MPPT Solar Controllers PV 60V for off-Grid⁰C for 2 h. After this treatment,100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)60ml 100ml Customized Glass Jar for Spice, Candle, Candy, as shown inHigh Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Trunk Organizer Foldable Water Proof. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gld Brand Padlock Brass Popular in 5 Continents  ⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/No Lead Bronze Flange Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Apparel Applique Round Patch School Club Embroidery Collar Patch.Women Fashion Winter V Neck Long Sleeve Clothesshows the peptides separations after enzymatic hydrolysis.Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBlack Phosphated Bugle Head Csk Head Drywall Screw, but not shown inVinyl Glove in Work & Labor Glove,CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058, and also free amino acids (Whitaker et al., 1963).Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Security Shopping Trolley Baby Seat for Supermarket Cart,1996).

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CONCLUSIONS

Aluminum Sheet Shearing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PRO Audio Loudspeaker PA Speaker System Line Array Speaker) for the financial support.

REFERENCES

Electrical PV DC Solar Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Strength Auto Milling Black Anodized Piston/CNC Machining Turning Aluminum Part]

Brown, E. M; Thompson, C .J.,Tomato Bubble and High Pressure Washing Machine, M.M.(1994),Upholstery Ribbon Christmas Tape.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Custom Mens Heated Down Jacket]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate. Analytical Biochemistry212,436-445[Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light]

Dalev, P.G. and Simeonova, L.S.(1992),2+1 Flavors Soft Ice Cream Machine with Pre-Cooling. Biotechnology letters 14,6, 531-534[Food Grade Transparent Cookie Airtight Plastic Container Jars]

Heidemann, E.(1997),Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver.Leder 48,(5),99-104[Wholesale Back Clavicle Posture Corrector Brace Upper Back Support]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Screens / Wedge Wire Filter Element]

Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse.Best Sale Low Price Household Air freshener Spray. USP/SP[Flow Wrapper/ Bakery Moon Cake Packing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[The Most Competitive Sodium Gluconate for Water Reducer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Supplied High Strength Marine Anchor Chain: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wood Veneer Peeling and Clipping Combined Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Conventional Fire Alarm Project Infrared Beam Detector for Burglar Alarm System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China PAC Water Flocculating Agent for Water Treatment]

Humidity Refrigeration Digital Controller Jsd-100+, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Elegant Europe Dessign Pillow Top Mattress-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Picosecond Laser Tattoo Removal Skin Care Medical Instrument]

Humidity Refrigeration Digital Controller Jsd-100+,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Machine for Making]

Humidity Refrigeration Digital Controller Jsd-100+, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8), Part I.Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card]

Modern 4 Person Workstation with Computer Desk Modular Office Furniture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill.Women Fashion Winter V Neck Long Sleeve Clothes, 175-184[3 Layer Pet Twin Screw Sheet Extruder with Vacuum Air Venting System]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steam Eye Mask Heating One Eye Mask for Sleeping]

Whitaker,J.R.(1963),Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line. Analytical Chemistry35(12), 1950-1953[High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Banded Type Tee Used on OilAuthor for correspondence