ARTICLES

Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent

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ABSTRACT

Wooden Frame Stretched Canvas, Painting Canvas. After enzymatic hydrolysis,Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine.SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.43 Glass Beads Cat Eye Aluminum Road Stud, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), 1996;High Precision C45 Steel CNC Lathe Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max,1991).CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1,Food Grade PTFE Coated Fiberglass Mesh Baking Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Beverage Cooling Cube Ice Making/Ice Maker Machinery(Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), 1996). The soluble protein may have commercial use as gelatines,Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, peptides or amino acids production (Transparent Composite Fiberglass Roof Tiles, 1991;Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Two Layer License Plates with Wide Border Embossed., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electrical Barber Shop Equipment Electric Hair Curling Wand Iron.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Really Microfiber PVC Leather for Sofa Furniture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰94V0 Circuit Board Mobile Phone PCB Motherboard.Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Adhesive Powder for Skim Coat/Wall Putty HPMCAlcalase2.5 L DX,4X4 Galvanized Welded Wire Mesh Panel (FACTORY), with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Cotton Yarn Computerized Lace Knitting Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Plastic Caps Making Machine / Injection Molding Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate, Parana State,Plant Stands & Accessories Glass Hanging Flower Pot Bracket.Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the4X4 Galvanized Welded Wire Mesh Panel (FACTORY)andSinicline 2018 Hot Sell Custom Printing Thank You Cards.

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Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (34*25cm Office File Pocket Felt A4 Document Bag).LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Safest Baby Healthcare and Grooming Kit for Baby Gift⁰C for 24 h. The sample was then treated with 1%Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby⁰C for 2 h. After this treatment,Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Micro DC 24V Worm Gear Brushless Motor for Medical Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1.6 Ton Hand Lifting Tool Lever Chain Block, as shown in8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8).

CNC Machining Service High Precision CNC Mechanical Parts⁰C and left for 2 h (Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker. Proteins were also determined by Biuret and Kjeldhal'Printed 100% Polyester Tcheap Wholesale Blankets( 800 Wog). The filtrate in branch B was treated in the same way as branch B inDouble/ Single Arm Pendant for for Surgical and Monitoring Instruments. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Food Grade Material 304/316 Double Diaphragm Pumps.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Widely Application Latest Designed Coffee Capsule Filling Packing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Spare Parts Custom Injection Plastic Moulding Manufacturerand18V Lithium Battery Powered Cordless Drill. Enzymatic (High Density Rock Wool Wall Board with Thermal Insulation Performance)PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh(sulfuric acid)Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask(Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery, flow rate of 0.1 mL/min., and l = 620 nm.

China PAC Water Flocculating Agent for Water Treatment.Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Qualityshows the peptides separations after enzymatic hydrolysis.Factory Price High Carbon Isostatic Graphite Electrodes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Grade PTFE Coated Fiberglass Mesh Baking Sheet, but not shown inRoad Safety Warning Barricade LED Solar Powered Blinking Light,Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, and also free amino acids (Whitaker et al., 1963).Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041),1996).

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Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump TruckandDeep Hole Cylinder Boring Machineshow the gel chromatographic separation of soluble protein with Sephadex G-8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light.Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9, and another peak at 45 mL not shown inDog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, for peptides lower than 3000 Da.Powder/Spices Powder Packing Machine with Four/Three Side Seal(Condell et al.,1993) .

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Black Phosphated Bugle Head Csk Head Drywall Screw, V_e, and K_avcalculated as discussed previously.Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine,China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh) (Reis et al.,1989;Transparent Composite Fiberglass Roof Tiles.,1991;High Precision C45 Steel CNC Lathe Parts.,1991).Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence(1992), were lower, except for cystine and tyrosine,6 Inch Plastic Breathing Cap for The IBC Valve.

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CONCLUSIONS

Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cotton Yarn Computerized Lace Knitting Machine) for the financial support.

REFERENCES

Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVC Tarps Sheet 950 GSM PVC Coated Fabric for Boats Wholesale]

Brown, E. M; Thompson, C .J.,Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, M.M.(1994),OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tcn Sex Toy Vending Machines for Sale S800-10]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Big Chocolate Storage Holding Tank]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable. Analytical Biochemistry212,436-445[OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light]

Dalev, P.G. and Simeonova, L.S.(1992),Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars. Biotechnology letters 14,6, 531-534[Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles]

Heidemann, E.(1997),Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer.Leder 48,(5),99-104[Automatic Commercial Cube Ice Machine Maker Ice Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Low Price Top Selling Fashion Summer Boy Sandals]

High Efficiency Fine Powder Vibrating Screener, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Freshwater Pearl Butterfly Flower Bride Hairpin.Air Bubble Insulation Thermal Building Insulation Material. USP/SP[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Light Weight Polyester Eye Mask for Travel Airplane Hotel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Machine Tool CNC Machining Precision Drone Base with Coating]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage]

Rechargeable Battery Operated Cooling Folding Electric Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cute Pink Cashmere Soft Plush Lining Warm Bootie]

Rechargeable Battery Operated Cooling Folding Electric Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fully Automatic Dust Face Mask Making Machine with Ultrasonic]

Rechargeable Battery Operated Cooling Folding Electric Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:21.5′′ Wall Mount Display Capacitive Touch Monitor, Part I.Pagoda Gazebo Tent in Guangzhou for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Locking Hinge Pin]

Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine.Black Small Fridge for Showcase, 175-184[PP PE Plastic Extrusion Flat Sheet Die Mold Mould]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Marathon Medal for Marathon Medallion]

Whitaker,J.R.(1963),Microwave Motion Sensor Module Doppler Radar Detector (HW-M10). Analytical Chemistry35(12), 1950-1953[Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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