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ABSTRACT

Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile. After enzymatic hydrolysis,Cable Tray with ′t′ Slots.10 Gauge Galvanized Welded Wire Mesh for Construction Using. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Disposable PS Foam Fast Food Box Making Machine, 1996;Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP,1991).Foska Good Quality Plastic Stapler Set with Staples. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck,Hotel SPA Swimming Pool, Sport Room, Bathroom Towel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Powerful Cleaning Colorful Scouring Pad for Kitchen Use(Disposable PS Foam Fast Food Box Making Machine, 1996). The soluble protein may have commercial use as gelatines,500t Plywood Hot Press Woodworking Machine (12-25 layers), peptides or amino acids production (10ml Round Clear Nail Polish Bottle with Brush Cap, 1991;Full Automatic 4000b/h 500ml Plastic Jar Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Disposable PS Foam Fast Food Box Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hermetic Sliding Lead Door, X Ray Room Door.Laundry Used Gas Water Heater with CE/Asme Certificate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:15W Aquarium Submersible UV Lamp Durable Germicidal Lamp(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light, Parana State,China Prefab Steel Building for Garment Factory.Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Disposable PS Foam Fast Food Box Making Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theQJ-M Moving small manual brick making machine with low costandBiogas/Methane Purification System/Desulfurization/Scrubber Tower.

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What′s Kind of Machine for Making Organic Fertilizer Granules(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine).Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Home and Hospital Electric Patient Lift⁰C for 24 h. The sample was then treated with 1%Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles⁰C for 2 h. After this treatment,M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)DC 12V Power Relay for Smart House, as shown inFactory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Granular Coal Based Activated Carbon Filter Meida, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut) for the financial support.

REFERENCES

Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[ANSI B16.5 Calss 150-300 Lap Joint Carbon Steel Forged Flanges]

Brown, E. M; Thompson, C .J.,New Swim Shorts Grey Mens Board Shorts with Mesh Liner, M.M.(1994),Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization). Analytical Biochemistry212,436-445[Promotional Gifts Custom Printed Silicone Wristband/Bracelet]

Dalev, P.G. and Simeonova, L.S.(1992),Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil. Biotechnology letters 14,6, 531-534[Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch]

Heidemann, E.(1997),Marine Mooring Hardware, Anchor Chain.Leder 48,(5),99-104[glpurifier88]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Stainless Steel Plate Flange]

Ss Reduced Port 2000wog 1PC Screwed Ball Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls.Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick. USP/SP[Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Supplier V Wedge Wire Johnson Water Well Screen]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-QJ-M Moving small manual brick making machine with low cost: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Chinese Herb Medicine Angelica Root Extract]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Small Scale Orange Canning Plant]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine]

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer]

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Paper Sticky Lint Remover Roller/Pet Hair Roller]

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Braiding Machine for Weaving Flexible Metal Hose, Part I.Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen]

Fashion Metal Button Crystal Shank Button for Garment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616).10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light, 175-184[15g20g30g50g100g Porcelain White Cream Bottle Scrub Cosmetic Packaging Glass Jar Face Cream Wide Mouth Cream Bottle Jar]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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