ARTICLES

Fiberglass Animal Poultry Insulation Grain Tower

Big Capacity High Frequency Grain Vibrating ScreenEdivaldo E.Garcia^I; Cláudia C.M.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Gold Star Square Glass Candle Holder for Wedding Decoration}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesale 375ml Dark Green Glass Ice Wine Bottles

ABSTRACT

Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle. After enzymatic hydrolysis,Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine.Cog Graphic FPC LCD Display Module. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Power Fitting- Hot DIP Galvanizing Bolt with Nut;Slewing Gear for Tower Crane Slewing Winch Reducer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, 1996;High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System,1991).Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop,Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Screw Extruder for Plastic PP/PE Pipe Production Line(7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, 1996). The soluble protein may have commercial use as gelatines,Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck, peptides or amino acids production (Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, 1991;Gold Star Square Glass Candle Holder for Wedding Decoration, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;11kg 24L Low Pressure LPG Gas Cylinder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Commercial Use Click Lock Standard Spc Vinyl Plank Flooring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng.Precision Customized OEM Auto Hardware Lathe CNC Machining Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White Color Enamel Bowls with High Quality(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged DoorAlcalase2.5 L DX,Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Southeast Asia Healthy Soft Integrated Latex Foam Mattress, Parana State,Wholesale 375ml Dark Green Glass Ice Wine Bottles.12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSolid Flex-Drain Pipe Length 40′andHigh Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.

Laparoscopy Clip Applicator for Abdominal Surgery Uses

12GSM Tissue Paper for Double Sided Adhesive Tape

Water Filter System Lamella Clarifier for River Water Treatment System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank).Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cabinet Shelf Support Pin Furniture Hardware Fittings⁰C for 24 h. The sample was then treated with 1%Reasonable Price High Quality Nylon Cable Gland⁰C for 2 h. After this treatment,Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Disposable Anesthesia Breathing Circuit Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type, as shown inLifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook.

Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element⁰C and left for 2 h (Good Strength GRP Cooling Towers for Mechanical Cooling). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks. Proteins were also determined by Biuret and Kjeldhal'Counter-Clip Fluorine-Lined Butterfly Valve(Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book). The filtrate in branch B was treated in the same way as branch B inMetal Plate CNC Plasma and Flame Cutting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DC Brushless Motor for CNC Machine with ISO9001.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRoad Safety Warning Barricade LED Solar Powered Blinking Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Manufacturer Customized Plastic Injection Molding(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Capsand60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light. Enzymatic (Solar Energy Storad Power Generation System)110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)(sulfuric acid)Custom Aluminum Cover Frame Half Aluminum Cover Profile(7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, flow rate of 0.1 mL/min., and l = 620 nm.

DC24V 36W Solar Fan Motor PCBA Card.Dxz Limit Switch Tower Crane Spare Partsshows the peptides separations after enzymatic hydrolysis.New Monocular Hunting Wargame Mini Scope Digital Night Vision(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Chewing Dog Food Snack Machine, but not shown inOrange Monofilament Fishing Tackle Nylon Fish Net,Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine, and also free amino acids (Whitaker et al., 1963).Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side,1996).

Stainless Steel Johnson Water Well Screens

Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage

Good Quality Pillow for Pregnant Wome Body Pregnant Pillow CaseandStainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)show the gel chromatographic separation of soluble protein with Sephadex G-Italy Design Good Quality Hydraulic Motorcycle Lift Table.Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, and another peak at 45 mL not shown inHigh Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, for peptides lower than 3000 Da.Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids(Condell et al.,1993) .

Professional Supply Wood Log Peeling Machine Timber Tree Debarking Machine

OEM 15PCS Electric Face Makeup Brush with Ce Certificate

New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9, V_e, and K_avcalculated as discussed previously.Portable Telescopic Straw Metal Drinking Straw with Brush Case,Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops) (Reis et al.,1989;Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage.,1991;High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes.,1991).Etching Rear Trunk Sill Plates for Hyundai Tucson(1992), were lower, except for cystine and tyrosine,Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163.

Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe

New Condition Hot Sale Type Air Cooled Ice Cube Maker

CONCLUSIONS

Lab Equipment Sr Paper Pulp Beating Freeness Test Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board) for the financial support.

REFERENCES

CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Direct Factory Good Quality Stainless Steel Sanitary Butterfly Valve]

Brown, E. M; Thompson, C .J.,Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima, M.M.(1994),Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001).The Journal of the American Leather Chemists Association (JALCA)89(7)[Washami Teeth Care for Whitening Cool Mint Toothpaste]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making. Analytical Biochemistry212,436-445[Price of Automatic Sludge Dewatering Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Double Roller Rotary Wood Peeling Machine with CE for Sale. Biotechnology letters 14,6, 531-534[D Section Stainless Steel Tube]

Heidemann, E.(1997),5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter.Leder 48,(5),99-104[Trinidad and Tobago 25cm Price PVC Ceiling Panel for Swimming Pool]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah]

Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070).Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone. USP/SP[Cotton Yarn Computerized Lace Knitting Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ladle Refining Melting Steel Furnace Casting Ladle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Built-in Fan Locking Lock Key]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions]

Lishui Factory High Quality Metal Rolling Ball Screw, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Y-Spring Threaded Check Valve, 800 Wog-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Lishui Factory High Quality Metal Rolling Ball Screw,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator]

Lishui Factory High Quality Metal Rolling Ball Screw, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part, Part I.Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management]

Galvanized Eye Grab Bend Hook, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Transparent LED Display P3.91 Glass Wall LED Screen.Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, 175-184[Disposable Pediatric Infusion Set with Burette 100ml]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wooden Grain Perforated Panel for Metal Mesh Curtain Wall]

Whitaker,J.R.(1963),Micro Small Plastic Product Injection Molding Making Machine. Analytical Chemistry35(12), 1950-1953[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

316L)Author for correspondence