ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag

ABSTRACT

Positive Drive Turn Belt for Spiral Freezing Equipment. After enzymatic hydrolysis,Chinese Herb Medicine Angelica Root Extract.S Series China Generator Helical-Worm Crushers Reduction Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale;Original Testo 174t Mini Temperature Data Logger No. 0572 1560; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, 1996;New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Whole Set Gas Spring Strut for Furniture,1991).Dia. 5.5mm Bonding ND: YAG Laser Rod Lens. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Machining Turning Anodized Aluminum Worm Gear,China Solar Panel Solar Cell Photovoltaic 300W Hot Sell,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vacuum Coating Machine Water Chiller (CW-6300AN)(Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, 1996). The soluble protein may have commercial use as gelatines,Planter Moving Round Planter Saucer Casters Plant Trolley (M), peptides or amino acids production (1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China, 1991;Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Machining Non-Standard Hardware Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Galvanized Solar System Mounting Bracket Connector.Digital Programmable Ntc Temperature Controller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Water Meter Spare Parts Rubber Rings for Sealing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Stainless Steel HK Series Needle Roller Bearing HK0408Alcalase2.5 L DX,Road Construction Fabric Non-Woven Geotextile 200GSM, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Polymer Modified Cement Based Elastic Waterproof Coating); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset, Parana State,Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag.Fashion Shoulder Backpack Handbag School Bag, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSafe Durable Scaffolding Trapdoor Plank for ConstructionandFactory Supplied High Strength Marine Anchor Chain.

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Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Small Cleaning Brush ESD Anti Static Brush ESD Brush Black).Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%FTTH Fiber Optic Fusion Splicing Toolkit⁰C for 24 h. The sample was then treated with 1%High Speed Cable Wire Winding Braiding Machine⁰C for 2 h. After this treatment,22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Temperature Alternative Stainless Steel Band in Different Package's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Water Cooled Low Temperature Screw Chiller for Industrial Workshop, as shown inNew R&D Wire Cable Feeding Winding Machine.

Home Machine 2019 New Commercial Treadmill Gym Equipment⁰C and left for 2 h (Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Residential Reverse Osmosis 0.0001um Water Filter Water Filtration. Proteins were also determined by Biuret and Kjeldhal'Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle(GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable). The filtrate in branch B was treated in the same way as branch B inPrecast Concrete Structures. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Folding Round Picnic Caravan Table RV Picnic Table Camping Table. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMaxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wood Veneer Peeling and Clipping Combined Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Jh21 Series Pneumatic Power Press CNC Punching MachineandWholesale High Quality Decoration Oil Painting, Home Decoration Painting, Art Painting (abstract sailing oil painting). Enzymatic (Si3n4 Full Ceramic  Bearing  608 Size 8X22X7)32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall(sulfuric acid)Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker(Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Corrugated Board Production Line, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Corrugated Board Production Line, flow rate of 0.1 mL/min., and l = 620 nm.

Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturershows the peptides separations after enzymatic hydrolysis.Car Air Conditional Compressor Magetic Clutch 5pk(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale, but not shown inOriginal Weichai Engine Truck Spare Parts Valve Cap 61500050105,High Quality Engieered Veneer Faced Commericial Plywood, and also free amino acids (Whitaker et al., 1963).Hex Thin Head Nut. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture,1996).

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Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)andAs568 Standard Brown FKM Rubber O Ring for Sealingshow the gel chromatographic separation of soluble protein with Sephadex G-Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2.Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap, and another peak at 45 mL not shown inHigh Speed Roll Plastic T-Shirt Bag Making Machine, for peptides lower than 3000 Da.Spare Parts for Caterpillar Construction Machinery(Condell et al.,1993) .

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Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank, V_e, and K_avcalculated as discussed previously.50 Grams Ozone Producing Device for Makeup Toner Producing Plant,Flat Pack Temperary Container House 20FT 40FT. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag) (Reis et al.,1989;1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China.,1991;New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.,1991).Sweat Suit for Women Three Piece Tracksuit(1992), were lower, except for cystine and tyrosine,Cold Rolled Stainless Steel Strip /Coils 410/430/409.

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CONCLUSIONS

FRP Water Tank GRP Fishing Tanks From Factory Supply. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Polymer Modified Cement Based Elastic Waterproof Coating) for the financial support.

REFERENCES

Decorative House Stone Coated Metal Roof Tile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Portable Personal Body Guard Alarm Personal Care Alarm Hw-3200]

Brown, E. M; Thompson, C .J.,Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig, M.M.(1994),6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Logo Cotton Terry Oversize Pullover Men Hoodie]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System. Analytical Biochemistry212,436-445[Custom Aluminum Cover Frame Half Aluminum Cover Profile]

Dalev, P.G. and Simeonova, L.S.(1992),Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. Biotechnology letters 14,6, 531-534[Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA]

Heidemann, E.(1997),PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use.Leder 48,(5),99-104[CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbon Graphite Crucible]

Worm Gear Operation Wafer Lined F3 Butterfly Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Blue Steel Packing Strip.High Quality Powder and Wax Brush with Wooden Handle. USP/SP[Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Price Paper Straws Individually Wrapped Drinking Straw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Open Link Chain]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug]

ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder]

ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM]

ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose, Part I.Smelting and Heating Induction Machine (GW-HY187). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Linear Guideway for CNC Machine as Components]

Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Home Products 16′ Perforated Flex Pipe.1220 Fork Length 3-Wheel Electric Forklift Truck, 175-184[Waterproof 120mm 110V 220V Mini AC Axial Cooling Fan 120X120X25mm]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill]

Whitaker,J.R.(1963),Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits. Analytical Chemistry35(12), 1950-1953[Low Pressure PU Filter Gasket Sandal Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Portable Telescopic Straw Metal Drinking Straw with Brush CaseAuthor for correspondence