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ABSTRACT

Pet Anti-Static Needled Mat. After enzymatic hydrolysis,Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine.OEM Sheet Metal Fabrication for Aluminum Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Worm Gear Operation Wafer Lined F3 Butterfly Valve, 1996;Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag,1991).Positive Drive Turn Belt for Spiral Freezing Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chinese Herb Medicine Angelica Root Extract,S Series China Generator Helical-Worm Crushers Reduction Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale(Worm Gear Operation Wafer Lined F3 Butterfly Valve, 1996). The soluble protein may have commercial use as gelatines,Original Testo 174t Mini Temperature Data Logger No. 0572 1560, peptides or amino acids production (Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water, 1991;Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Whole Set Gas Spring Strut for Furniture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dia. 5.5mm Bonding ND: YAG Laser Rod Lens. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Worm Gear Operation Wafer Lined F3 Butterfly Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Machining Turning Anodized Aluminum Worm Gear.China Solar Panel Solar Cell Photovoltaic 300W Hot Sell-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vacuum Coating Machine Water Chiller (CW-6300AN)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Galvanized Solar System Mounting Bracket Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)).Digital Programmable Ntc Temperature Controller(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Water Meter Spare Parts Rubber Rings for Sealing⁰C for 24 h. The sample was then treated with 1%Stainless Steel HK Series Needle Roller Bearing HK0408⁰C for 2 h. After this treatment,Road Construction Fabric Non-Woven Geotextile 200GSM. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Polymer Modified Cement Based Elastic Waterproof Coating's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe, as shown inJqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay.

Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset⁰C and left for 2 h (Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Shoulder Backpack Handbag School Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl(Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door). The filtrate in branch B was treated in the same way as branch B inWater and Oxygen Water Spray Jet Facial Skin Rejuvenation Peel Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,FTTH Fiber Optic Fusion Splicing Toolkit.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Speed Cable Wire Winding Braiding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Water Cooled Low Temperature Screw Chiller for Industrial Workshop⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Home Machine 2019 New Commercial Treadmill Gym Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Mass Stock Yl10.2 Carbide Rods for Milling 45HRCandResidential Reverse Osmosis 0.0001um Water Filter Water Filtration. Enzymatic (Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle)China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses(sulfuric acid)Folding Round Picnic Caravan Table RV Picnic Table Camping Table(Worm Gear Operation Wafer Lined F3 Butterfly Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, flow rate of 0.1 mL/min., and l = 620 nm.

3HP Industrial Cooling System Water Cooled Chiller.Size 000 Clear Pullulan Vegetarian Capsulesshows the peptides separations after enzymatic hydrolysis.OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEndoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments, but not shown inOutdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale,Wood Veneer Peeling and Clipping Combined Machine, and also free amino acids (Whitaker et al., 1963).Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Worm Gear Operation Wafer Lined F3 Butterfly Valve,1996).

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Sodium Bicarbonate Polifar

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CONCLUSIONS

Hex Thin Head Nut. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)) for the financial support.

REFERENCES

Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Multi-Function Super Sodium Humate]

Brown, E. M; Thompson, C .J.,Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap, M.M.(1994),Spare Parts for Caterpillar Construction Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[PRO Audio Loudspeaker PA Speaker System Line Array Speaker]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank. Analytical Biochemistry212,436-445[Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker]

Dalev, P.G. and Simeonova, L.S.(1992),50 Grams Ozone Producing Device for Makeup Toner Producing Plant. Biotechnology letters 14,6, 531-534[Home Products 16′ Perforated Flex Pipe]

Heidemann, E.(1997),Flat Pack Temperary Container House 20FT 40FT.Leder 48,(5),99-104[Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hex Thin Head Nut]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-FRP Water Tank GRP Fishing Tanks From Factory Supply: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automotive Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails]

Decorative House Stone Coated Metal Roof Tile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad]

Decorative House Stone Coated Metal Roof Tile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Decorative House Stone Coated Metal Roof Tile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder, Part I.Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use.Blue Steel Packing Strip, 175-184[Cascade K Series Fork Positioner]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anodized Aluminum Expanded Metal Mesh Competitive Price]

Whitaker,J.R.(1963),High Quality Powder and Wax Brush with Wooden Handle. Analytical Chemistry35(12), 1950-1953[DC24V 36W Solar Fan Motor PCBA Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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