ARTICLES

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ABSTRACT

Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization). After enzymatic hydrolysis,Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil.Marine Mooring Hardware, Anchor Chain. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.QJ-M Moving small manual brick making machine with low cost, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, 1996;Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Braiding Machine for Weaving Flexible Metal Hose,1991).Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Metal Button Crystal Shank Button for Garment,Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light(Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, 1996). The soluble protein may have commercial use as gelatines,Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, peptides or amino acids production (ABC-508 Brake System Cleaning and Fluid Exchanging Machine, 1991;Polypropylene Woven Bag for Sludge Dewatering, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Industrial Grade Anhydrous Sodium Sulfate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Factory Supply Design Cat Tree Pet Product.Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Peb Steel Structure Building Materials H-Section I Beam Steel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Famous Brand Luxury Packaging Box Gift Box, Parana State,Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control.High Qualified Medical Grade Thin Wall Stainless Steel Tube, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCeramic Edge Protection Profiles Decorative Ceramic Metal Tile TrimandFly.

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3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cable Tray with ′t′ Slots).High Strength New 0.18mm Molybdenum Wire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Structural Synthetic Fiber for Concrete⁰C for 24 h. The sample was then treated with 1%LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer⁰C for 2 h. After this treatment,Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tct Circular Saw Blade for Grass Cutting, as shown inRefrigeration Digital Temperature Controller Stc-100A.

Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet⁰C and left for 2 h (Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Picc Puncture Care Kit. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth. Proteins were also determined by Biuret and Kjeldhal'AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture(Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank). The filtrate in branch B was treated in the same way as branch B inVitamin H. The final soluble protein shown were used for peptides separations using gel permeation chromatography,34*25cm Office File Pocket Felt A4 Document Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PP Needlepunched Nonwoven Geotextile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Temperaturethermocouple Male and Female Plug(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Price Custom Embroidery Logo Hotel Slippers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Efficiency Fine Powder Vibrating Screener, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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UF Membrane Aqu160 with Competitive Price and High Quality.Color Change LED Restaurant Dinner Chair and Table Restaurant Furnitureshows the peptides separations after enzymatic hydrolysis.Atf-805 Auto Car Transmission System Flushing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCountersunk Head Self Drilling Screw Color Zinc Plated DIN7504r, but not shown inSweat Suit for Women Three Piece Tracksuit,43 Glass Beads Cat Eye Aluminum Road Stud, and also free amino acids (Whitaker et al., 1963).Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device,1996).

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CONCLUSIONS

Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine) for the financial support.

REFERENCES

Two Layer License Plates with Wide Border Embossed, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China digital scale weighs baggage suitcases]

Brown, E. M; Thompson, C .J.,Electrical Barber Shop Equipment Electric Hair Curling Wand Iron, M.M.(1994),Really Microfiber PVC Leather for Sofa Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Yield Tree Stump Crusher Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-94V0 Circuit Board Mobile Phone PCB Motherboard. Analytical Biochemistry212,436-445[Water Meter Spare Parts Rubber Rings for Sealing]

Dalev, P.G. and Simeonova, L.S.(1992),Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers. Biotechnology letters 14,6, 531-534[Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)]

Heidemann, E.(1997),PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve.Leder 48,(5),99-104[6fo 12fo Fiber Optic Patch Cord]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Construction Welding Rods Electrode E6013 E7018 E7016]

Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Adhesive Powder for Skim Coat/Wall Putty HPMC.4X4 Galvanized Welded Wire Mesh Panel (FACTORY). USP/SP[10ml Round Clear Nail Polish Bottle with Brush Cap]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cotton Yarn Computerized Lace Knitting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial SUS 304 Fruit Washing Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Machining Turning Anodized Aluminum Worm Gear]

Plastic Caps Making Machine / Injection Molding Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve]

Plastic Caps Making Machine / Injection Molding Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Graphic 128X128 Dots Cog LCD]

Plastic Caps Making Machine / Injection Molding Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge, Part I.Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor]

LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Safest Baby Healthcare and Grooming Kit for Baby Gift.Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, 175-184[Bale Gripper Loader Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale 25kw Grid Tied Home Solar Panel System Kit]

Whitaker,J.R.(1963),Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro. Analytical Chemistry35(12), 1950-1953[Manual Interlocking Brick Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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