ARTICLES

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ABSTRACT

Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder. After enzymatic hydrolysis,Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52.China Supplier Granular Coal Based Activated Carbon Filter Meida. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Glass Cutting Table/CNC Glass Cutting Machine;12 Bottles Portable Home Ues Thermoelectric Wine Chiller; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), 1996;Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening,1991).Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe,Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve(Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), 1996). The soluble protein may have commercial use as gelatines,Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron, peptides or amino acids production (Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, 1991;High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hanwha SMT Chip Shooter LED Pick and Place Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ACP Aluminium Cmposite Panel Cladding Extrusions.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Custom Mens Heated Down Jacket.Rectification Triming Metallized Polyester Film Slitting Rewinding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Loquat Juice and Loquat Syrup Production Machinery Export Worldwide).QJ-M Moving small manual brick making machine with low cost(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device⁰C for 24 h. The sample was then treated with 1%Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set⁰C for 2 h. After this treatment,Braiding Machine for Weaving Flexible Metal Hose. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Metal Button Crystal Shank Button for Garment, as shown in12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery.

Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616)⁰C and left for 2 h (Reasonable Price High Quality Nylon Cable Gland). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching. Proteins were also determined by Biuret and Kjeldhal'ABC-508 Brake System Cleaning and Fluid Exchanging Machine(VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)). The filtrate in branch B was treated in the same way as branch B inCmc For Drilling Fluid. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Polypropylene Woven Bag for Sludge Dewatering.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Professional Factory Supply Design Cat Tree Pet Product⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Peb Steel Structure Building Materials H-Section I Beam Steel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Folding Round Picnic Caravan Table RV Picnic Table Camping TableandAluminum Oxide Non Woven Polishing Wheel for Machine. Enzymatic (CAT6A FTP Patch Panel (WD6A-012))LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine(sulfuric acid)Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant(Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Famous Brand Luxury Packaging Box Gift Box, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Famous Brand Luxury Packaging Box Gift Box, flow rate of 0.1 mL/min., and l = 620 nm.

High Qualified Medical Grade Thin Wall Stainless Steel Tube.Walking Long Distance Surveying Instrument Meter Measuring Wheelshows the peptides separations after enzymatic hydrolysis.3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMicro DC 24V Worm Gear Brushless Motor for Medical Equipment, but not shown inCarboxymethyl Cellulose CAS No. 9004-32-4,High Strength New 0.18mm Molybdenum Wire, and also free amino acids (Whitaker et al., 1963).Structural Synthetic Fiber for Concrete. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321),1996).

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Tct Circular Saw Blade for Grass Cutting, V_e, and K_avcalculated as discussed previously.Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet,Picc Puncture Care Kit. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service) (Reis et al.,1989;Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch.,1991;Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap.,1991).Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth(1992), were lower, except for cystine and tyrosine,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.

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CONCLUSIONS

34*25cm Office File Pocket Felt A4 Document Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)) for the financial support.

REFERENCES

PP Needlepunched Nonwoven Geotextile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Metal Forming Tools]

Brown, E. M; Thompson, C .J.,High Temperaturethermocouple Male and Female Plug, M.M.(1994),34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Made in China Lqa Strap Banding Tool]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Price Custom Embroidery Logo Hotel Slippers. Analytical Biochemistry212,436-445[China Modern Pharmacy Vacuum Filter Press]

Dalev, P.G. and Simeonova, L.S.(1992),Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent. Biotechnology letters 14,6, 531-534[Folding Play Mat Crawl Play Mat]

Heidemann, E.(1997),High Efficiency Fine Powder Vibrating Screener.Leder 48,(5),99-104[2018 New Type Cordless Drill 18V Li-Battery Power Drill and Power Impact]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dbry-320 Christmas Stickers Labels Printing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fiber Optic Sc APC Fast Connector for FTTH Drop Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wooden Frame Stretched Canvas, Painting Canvas: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[20ft/40ft Container Homes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Foil PE Bottle Cap Induction Seal Liner]

Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Racing Preformance PTFE Teflon Alloy Fuel Adapter]

Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length]

Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:UF Membrane Aqu160 with Competitive Price and High Quality, Part I.Atf-805 Auto Car Transmission System Flushing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Robot of World Robotics Competition]

43 Glass Beads Cat Eye Aluminum Road Stud, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041).High Precision C45 Steel CNC Lathe Parts, 175-184[Two Layer Inner Tube Tire Curing Press Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2mm Mirror Glass/Coated Glass for LED]

Whitaker,J.R.(1963),I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max. Analytical Chemistry35(12), 1950-1953[Food Machine Cube Ice Machine for Commercial Use]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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