ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Nylon Weatherproof PA6 Strain Relief Cable Gland. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Steel Roller Embossed Paper 2019 New Design

ABSTRACT

Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil. After enzymatic hydrolysis,Factory Price DIN5685 Galvanized Marine Anchor Link Chain.Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck;High Level Application Waterproof Plastic Box; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, 1996;Water Slide for Indoor Play Structure., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder,1991).1220*2440mm Concrete Formwork black film faced plywood. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Washami Teeth Care for Whitening Cool Mint Toothpaste,Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine(Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, 1996). The soluble protein may have commercial use as gelatines,SHXJ1500 CNC Diamond Wire Stone Cutting Machine, peptides or amino acids production (Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag, 1991;2019 Foam Concrete Machine/Foamed Concrete Block Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Yyh Series One Dimension Tumbling Mixer Mixing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ODU 14 Pin Industrial Circular Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board.Classical New Coming Plastic Female Sunglasses-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Double Sided Rigid PCB Circuit Board Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, Parana State,Steel Roller Embossed Paper 2019 New Design.China Supplier Steel Machine Bellows Cover Telescopic Shield Cover, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber PartsandCopy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine.

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Concrete Cure Spray Concrete Curing Agent(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Air Bubble Insulation Thermal Building Insulation Material).Automatic Chewing Dog Food Snack Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%White Ceramic Shot Glass for Sublimation Printing - 1.5oz⁰C for 24 h. The sample was then treated with 1%Promotional Gifts Custom Printed Silicone Wristband/Bracelet⁰C for 2 h. After this treatment,Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Farrowing Crate Pig Floor Cast Iron Slat Floor, as shown inWholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer.

Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing⁰C and left for 2 h (Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Aluminum Heat Sink for Electronics Computer Electric. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover. Proteins were also determined by Biuret and Kjeldhal'Surprise! Range Hood Free! ! ! Juice Cart(Various Colors Nylon and Stainless Steel Kitchen Tools). The filtrate in branch B was treated in the same way as branch B inm.outdoorgamefactory.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Steam Eye Mask Heating One Eye Mask for Sleeping.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic French Fries Frying Machine with Low Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSolar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325)andHigh Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card. Enzymatic (T90 High Power Latching Relay Mini Power Relay)Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch(sulfuric acid)Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430(Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Polyglycolic Acid Absorable Suture, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Polyglycolic Acid Absorable Suture, flow rate of 0.1 mL/min., and l = 620 nm.

No. 1062 20PCS Tools.Factory Outlet Automatic Grain Tower Dryer Drying Equipmentshows the peptides separations after enzymatic hydrolysis.Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality CE Digital Display Adjustable Power Supply, but not shown inAuto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430,Aluminum Mould Shoe Mould Making CNC Milling Router Machine, and also free amino acids (Whitaker et al., 1963).High Pressure Fitting Quick Coupling Hydraulic Plug. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse,1996).

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White Cotton Satin Hotel Duvet Cover (DPFB8055)andCtg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Lineshow the gel chromatographic separation of soluble protein with Sephadex G-High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover.Refrigeration Digital Temperature Controller Stc-100A, and another peak at 45 mL not shown inCold Rolled Stainless Steel Strip /Coils 410/430/409, for peptides lower than 3000 Da.Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle(Condell et al.,1993) .

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Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies, V_e, and K_avcalculated as discussed previously.Black Color Hexagon Bolt with High Quality,China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home) (Reis et al.,1989;Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.,1991;Water Slide for Indoor Play Structure.,1991).Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price(1992), were lower, except for cystine and tyrosine,Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag.

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CONCLUSIONS

High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum PCB Circuit Board SMD PCBA LED PCB) for the financial support.

REFERENCES

Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3d Brick Self-adhesive Wallpaper]

Brown, E. M; Thompson, C .J.,Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery, M.M.(1994),Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042).The Journal of the American Leather Chemists Association (JALCA)89(7)[Multi-Functional Backpack Manual 52cc Brush Cutter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 New Portable Mini Dental X-ray Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM. Analytical Biochemistry212,436-445[Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line]

Dalev, P.G. and Simeonova, L.S.(1992),Single Stage High Speed Fruit and Vegetable Pulping Machine. Biotechnology letters 14,6, 531-534[Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products]

Heidemann, E.(1997),China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.Leder 48,(5),99-104[Metal Roof Panel Roll Forming Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vulcanizing Machine /Rubber Tiles Vulcanzing Press]

Low Energy Needed Compact Flour Mill Plant for Milling Wheat, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services.Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads. USP/SP[Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Farrowing Crate Pig Floor Cast Iron Slat Floor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Amazon Armor TPU Silicone Phone Case for Honor 10 Lite]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[SS316 Steel Strut U Channel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous]

Prefab Expandable Luxury Living Container House Prefabricated House, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Movable Partition Walls for Room Division, Hotel and Shopping Mall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes]

Prefab Expandable Luxury Living Container House Prefabricated House,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine]

Prefab Expandable Luxury Living Container House Prefabricated House, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Carboxy Terminal Collagen Crosslinks Elisa Kits, Part I.Various Colors Nylon and Stainless Steel Kitchen Tools. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Power Fitting- Hot DIP Galvanizing Bolt with Nut]

355nm UV Laser Marking Equipment for Crystal Materials, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Metal Separating Gold Brass Zippers for Sale.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 175-184[sunybiz.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture]

Whitaker,J.R.(1963),OEM Refrigeration Digital Thermometer. Analytical Chemistry35(12), 1950-1953[ Shampoo Mascara Titling Mixing and Emulsifying Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pure Sine Wave Power Frequency InverterAuthor for correspondence