ARTICLES

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ABSTRACT

Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring. After enzymatic hydrolysis,48V25A Lead Acid Battery Maintenance Pulse Charger.15mm Best Price Commercial Grade Birch Plywood for Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Top Great Wallet Genuine Leather Purse with Coin Pocket;Good Price 20kVA Single Phase Silent Diesel Generator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals, 1996;Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service,1991).Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail,High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Clarifier Tank for Water Treatment(Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals, 1996). The soluble protein may have commercial use as gelatines,Carrier Roller for Sany Excavator, peptides or amino acids production (LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, 1991;Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle.High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable).New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855⁰C for 24 h. The sample was then treated with 1%CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc⁰C for 2 h. After this treatment,Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)AISI 201/202/301/304 Stainless Steel Welded Square Pipe, as shown inCNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts.

Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce⁰C and left for 2 h (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger. Proteins were also determined by Biuret and Kjeldhal'PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle(High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage). The filtrate in branch B was treated in the same way as branch B in1000l Alcohol Column Still. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Speed Copper Wire Braiding Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSafe Durable Construction Scaffolding Plank for Building(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Different Size of Ring NdFeB Magnet(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Welding Structure, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Carboxymethyl Cellulose CAS No. 9004-32-4, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer.from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwardershows the peptides separations after enzymatic hydrolysis.Vulcanizing Machine /Rubber Tiles Vulcanzing Press(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Speed Cable Wire Winding Braiding Machine, but not shown inCustom Size Christmas Gift Personalized Watch Boxes for Men,Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low, and also free amino acids (Whitaker et al., 1963).Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals,1996).

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Advanced Unlocking Tool for Locksmith) for the financial support.

REFERENCES

As568 Standard Brown FKM Rubber O Ring for Sealing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Horizontal Double Ribbon Mixer for Dry Powder/Wood Plastic Mixing Machine/20 Ton Horizontal High Speed]

Brown, E. M; Thompson, C .J.,The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent, M.M.(1994),Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminum Mould Shoe Mould Making CNC Milling Router Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-200W UFO LED High Bay Light Fixture for Interior Exterior Applications. Analytical Biochemistry212,436-445[Low Energy Needed Compact Flour Mill Plant for Milling Wheat]

Dalev, P.G. and Simeonova, L.S.(1992),China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2). Biotechnology letters 14,6, 531-534[Sower Vacuum Emulsifying Machine for Cosmetic]

Heidemann, E.(1997),13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder.Leder 48,(5),99-104[Double Deck High Quality Aluminum Pontoon Boat for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability]

Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bathroom False Plastic Board Interior Decorative 3D Wall Panels.Mushroom Head Stud Sert. USP/SP[Best Quality Bending Metal Mould with All Kinds Metal Materials]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2 Ton Air Cooled Water Chiller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Borosilicate Glass Baking Dish Set Clear-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding]

Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Shoulder Backpack Handbag School Bag]

Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, Part I.Embossing Roller for Tissue Paper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope]

Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates.Colorful  Reflective Sticker Reflective Tape, 175-184[Aramid Fabric Workwear]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Security Shopping Trolley Baby Seat for Supermarket Cart]

Whitaker,J.R.(1963),Concrete Spare Part Electrical System Central Distribution Box. Analytical Chemistry35(12), 1950-1953[Silver Anodized Aluminium I Beam Level Measuring Tool]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal MeshAuthor for correspondence