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ABSTRACT

High Quality CE Digital Display Adjustable Power Supply. After enzymatic hydrolysis,Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires.Parcel Tape Adhesive OPP Packing Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Striped Compression Soccer Socks for Adults, 1996;Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen,1991).High Tensile Strength Galvanized Steel Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,24 Volt Fire Alarm Strobe Light Horn Strobe Sounder,from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Price PVC Coated Fiberglass Mesh Insect Window Screen(Custom Striped Compression Soccer Socks for Adults, 1996). The soluble protein may have commercial use as gelatines,Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, peptides or amino acids production (Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor, 1991;Factory OEM CNC Machined Gear Motor Shaft, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pink French Loop Terry Knit Fabric for Jacket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Striped Compression Soccer Socks for Adults, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts.500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Sofa Materials PU Leather with Flock Backing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Positive Drive Turn Belt for Spiral Freezing Equipment).Three Phase 800mm Plate Fan(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ASTM Approved Popular Indoor Trampoline of Park⁰C for 24 h. The sample was then treated with 1%Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building⁰C for 2 h. After this treatment,Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Genuine Leather Credit Card Mens Wallet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yitai Die Making 13mm Beech Rotary Plywood, as shown inYitai Die Making 13mm Beech Rotary Plywood.

Gear Rolling Machine Zh28/25 Spline Hobbing Machine⁰C and left for 2 h (Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale. Proteins were also determined by Biuret and Kjeldhal'Partial Thread Steel/Iron Hex Head Self Tapping Screw(PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle). The filtrate in branch B was treated in the same way as branch B inhigh grade best LVL for construction. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flat Car Sun Roof Tent Awning.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNeutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pneumatic Control Shuttle Valve NPT Thread⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM 15PCS Electric Face Makeup Brush with Ce Certificate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing MachineandMotorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Blockshow the gel chromatographic separation of soluble protein with Sephadex G-Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules.Dbry-320 Christmas Stickers Labels Printing Machine, and another peak at 45 mL not shown inLarge Slewing Rings Bearings for Wind Turbine Ring on Sale, for peptides lower than 3000 Da.Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor(Condell et al.,1993) .

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CONCLUSIONS

High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Aluminum Bright Polished Mirror Sheet, M.M.(1994),Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open).The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Menthol E Liquid E Juice for EGO E Cigarettes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory OEM CNC Machined Gear Motor Shaft]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Motorcycle Reflector Injection Plastic Parts Mould Maker. Analytical Biochemistry212,436-445[High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton]

Dalev, P.G. and Simeonova, L.S.(1992),O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W. Biotechnology letters 14,6, 531-534[ COB Stn LCD Display with 8080 Interface]

Heidemann, E.(1997),Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China.Leder 48,(5),99-104[CNC Cutting Alloy Wheel Repair Lathe Car Wheel Rim Equipment Repair Rim Straightening Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Popular Custom School Uniform Woven Badge Patch with Merrow Border]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pump Flower Sprinkler with Automatic Watering System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Reliable Supplier Decorative Nut And Bolt - Hex nut - Jiangyu ]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rear Wheel Exchangeable Aluminum Manual Wheelchair]

Molasses Fuel Ethanol Distillation Plant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pond Self Priming Booster Pressure Water Pump with Check Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Body Infrared Burner Good Selling Gas Stove]

Molasses Fuel Ethanol Distillation Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags]

Molasses Fuel Ethanol Distillation Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Banded Type Tee Used on Oil, Part I.20FT New Shipping Container 40FT Dry Cargo Container. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Socket Flange]

High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone.High Pressure Braided Rubber Oil Hose, 175-184[Aluminum Die Casting Automobile Parts Custom Auto Heatsink Aluminum Die Casting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Disposable Sterile Twist Top Blood Lancet]

Whitaker,J.R.(1963),Monochrome Graphic Industrial Control LCD Display Module 128X64. Analytical Chemistry35(12), 1950-1953[Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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