ARTICLES

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ABSTRACT

Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor. After enzymatic hydrolysis,Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC.Explosion-Proof Housing Sealing Dispensing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tube Mills for Thin Wall Tubing;Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Car Badge Custom Emblem Auto Logo Sign for Advertising, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt, 1996;Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006,1991).Rear Wheel Exchangeable Aluminum Manual Wheelchair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow,Graphic 128X128 Dots Cog LCD,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen(2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt, 1996). The soluble protein may have commercial use as gelatines,Tcn Sex Toy Vending Machines for Sale S800-10, peptides or amino acids production (Silver 8 Inch Double Thick Round Cake Board, 1991;High Precision Machining Auto Parts Metal Contact, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Paper Sticky Lint Remover Roller/Pet Hair Roller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Safe Durable Scaffolding Trapdoor Plank for Construction.China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Various Sublimation Blank Home Supply Fabric Bath Towel etc(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus, Parana State,Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale.Milling Precision Stainless Steel CNC Machinery Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the10 Gauge Galvanized Welded Wire Mesh for Construction UsingandHigh Tensile Strength Galvanized Steel Wire.

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U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V).Good Price Stand-up Zipper Doy Pouch Filling Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21⁰C for 24 h. The sample was then treated with 1%Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10⁰C for 2 h. After this treatment,360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Food Machine Cube Ice Machine for Commercial Use, as shown inCustom Made Fabrication Service Anodized Aluminium CNC Machining Parts.

Christmas Decoration Rose Flower LED Light String⁰C and left for 2 h (on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.D Section Stainless Steel Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mint Flavor 50ml Home Foam Toothpaste. Proteins were also determined by Biuret and Kjeldhal'125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile(Promotional Gifts Custom Printed Silicone Wristband/Bracelet). The filtrate in branch B was treated in the same way as branch B inPrimary School Uniform Designs Cable-Knit V-Neck Pullover for Boys Girls. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dxz Limit Switch Tower Crane Spare Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh-End Business Magnet Leather Ring Binder Notebook(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Eyes- Caring LED Night Light for Nursing Reading with Soft Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Bearing with Housing Bearing / Pillow Block Bearing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane SwitchandSteel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800. Enzymatic (The Most Competitive Sodium Gluconate for Water Reducer)Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles(sulfuric acid)Electric Magic Hot Hair Styling Curling Iron with Brushes(2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Melt Pressure Transducer (PT133), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Melt Pressure Transducer (PT133), flow rate of 0.1 mL/min., and l = 620 nm.

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Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding MachineandEnergy Saving Small Air Conitioner Air Source Heat Pump Manufacturershow the gel chromatographic separation of soluble protein with Sephadex G-Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662.Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, and another peak at 45 mL not shown inPaper Sticky Lint Remover Roller/Pet Hair Roller, for peptides lower than 3000 Da.Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck(Condell et al.,1993) .

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CONCLUSIONS

Hotel Steel Digital Room Safes with Large LED Display. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube) for the financial support.

REFERENCES

Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Screw Cap Cosmetic Aluminum Lid for Glass Plastic Bottle]

Brown, E. M; Thompson, C .J.,Biogas/Methane Purification System/Desulfurization/Scrubber Tower, M.M.(1994),Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Popular Custom School Uniform Woven Badge Patch with Merrow Border. Analytical Biochemistry212,436-445[Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone]

Dalev, P.G. and Simeonova, L.S.(1992),1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor. Biotechnology letters 14,6, 531-534[Pneumatic Control Shuttle Valve NPT Thread]

Heidemann, E.(1997),Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter.Leder 48,(5),99-104[Full Metal Large Printing Size Professional Dual Extruder 3D Printer Metal De Plus]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Fashion PVC Sequins Adjustable Women Brace Suspenders]

Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electrical Stationary Hoist Tower Crane 500kg Motor.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage. USP/SP[ Synergist for Soap; Water Softener]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Eye Steam Mask]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Jolink Black Masterbatch (2050c) for Cable/ Pipe/ Sheet Extrusion]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Carrier Roller for Sany Excavator]

Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flow Wrapper/ Bakery Moon Cake Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot DIP Galvanized G2150 D Ring Shackle]

Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminum Oxide Non Woven Polishing Wheel for Machine]

Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, Part I.Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Concrete Floor Grinding Disc Machine for Four Segments]

Reusable Metal Stainless Steel Drinking Straws, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Designed Steel Frame Factory Building.Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate, 175-184[Customized Plastic Products Components Auto Parts Machine Parts Plastic Gear Wheel Injection Moulding]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile]

Whitaker,J.R.(1963),0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table. Analytical Chemistry35(12), 1950-1953[Planter Moving Round Planter Saucer Casters Plant Trolley (M)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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