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ABSTRACT

Hot DIP Galvanized G2150 D Ring Shackle. After enzymatic hydrolysis,Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair.Round Shape Aluminum PCB and PCBA. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chicken Cage Mesh Hexagonal Wire Mesh Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (110/220V Handle Mild Steel Welding Machine PCB Board 160A, 1996;Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Enamel Cast Iron Mini Casserole Cast Iron Cocotte,1991).Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer,Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine(110/220V Handle Mild Steel Welding Machine PCB Board 160A, 1996). The soluble protein may have commercial use as gelatines,CNC System Air Duct Line5 Make Machine for HVAC, peptides or amino acids production (Titanium Dioxide Anatase for Paper Making, 1991;40A C Electronic Air Circuit Breaker with Dz47-4p, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pipe Thermostat with Sensor Ce Certificate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Walking Long Distance Surveying Instrument Meter Measuring Wheel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chinese Supplier of PVC Profile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (110/220V Handle Mild Steel Welding Machine PCB Board 160A, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tianjin Manufacturer PTFE Coated Butterfly Valve.Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Prices of 20W 40W 60W Solar Street Lights(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay).Pet Anti-Static Needled Mat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine⁰C for 24 h. The sample was then treated with 1%OEM Sheet Metal Fabrication for Aluminum Parts⁰C for 2 h. After this treatment,Hwf Tsp Core Bit for Geotechnical Drilling. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Price of Automatic Sludge Dewatering Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine, as shown inHolland Welded Wire Mesh Euro Fencing /Euro Fence for Garden.

Worm Gear Operation Wafer Lined F3 Butterfly Valve⁰C and left for 2 h (Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag. Proteins were also determined by Biuret and Kjeldhal'Positive Drive Turn Belt for Spiral Freezing Equipment(Mass Stock Yl10.2 Carbide Rods for Milling 45HRC). The filtrate in branch B was treated in the same way as branch B inCompetitive Price 4y Engine Spark Plug for Parts Toyota Hilux OEM W16ex-U 90919-01059. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Chinese Herb Medicine Angelica Root Extract.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%S Series China Generator Helical-Worm Crushers Reduction Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Planter Moving Round Planter Saucer Casters Plant Trolley (M).Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instrumentsshows the peptides separations after enzymatic hydrolysis.1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOutdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, but not shown inSelf Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic,Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X), and also free amino acids (Whitaker et al., 1963).Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (110/220V Handle Mild Steel Welding Machine PCB Board 160A,1996).

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Digital Programmable Ntc Temperature Controller, V_e, and K_avcalculated as discussed previously.Water Meter Spare Parts Rubber Rings for Sealing,Stainless Steel HK Series Needle Roller Bearing HK0408. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket) (Reis et al.,1989;Titanium Dioxide Anatase for Paper Making.,1991;Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming.,1991).Road Construction Fabric Non-Woven Geotextile 200GSM(1992), were lower, except for cystine and tyrosine,High Polymer Modified Cement Based Elastic Waterproof Coating.

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CONCLUSIONS

Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag) for the financial support.

REFERENCES

Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baby Food Eat Feeding High Dining Chair]

Brown, E. M; Thompson, C .J.,Fashion Shoulder Backpack Handbag School Bag, M.M.(1994),Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl. Analytical Biochemistry212,436-445[Home Products 16′ Perforated Flex Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),FTTH Fiber Optic Fusion Splicing Toolkit. Biotechnology letters 14,6, 531-534[Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608]

Heidemann, E.(1997),High Speed Cable Wire Winding Braiding Machine.Leder 48,(5),99-104[Wholesale 7inch Industrial Tablet Computer Naked Resistance Touch LCD Shell-Less]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Groove Type Wood Log Tree Peeling and Debarking Machine]

Factory Direct Sale Single Cloth Hanger Hook, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill.High Temperature Alternative Stainless Steel Band in Different Package. USP/SP[Hex Thin Head Nut]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Water Cooled Low Temperature Screw Chiller for Industrial Workshop: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Pizza Packing Box]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad]

Home Machine 2019 New Commercial Treadmill Gym Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet]

Home Machine 2019 New Commercial Treadmill Gym Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

Home Machine 2019 New Commercial Treadmill Gym Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Residential Reverse Osmosis 0.0001um Water Filter Water Filtration, Part I.Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anodized Aluminum Expanded Metal Mesh Competitive Price]

China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Folding Round Picnic Caravan Table RV Picnic Table Camping Table.Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, 175-184[P3.91 P4.81 Indoor/Outdoor Rental Floor LED Display Video Screen LED Wall for Wedding Living Show and Exhibition]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DC24V 36W Solar Fan Motor PCBA Card]

Whitaker,J.R.(1963),3HP Industrial Cooling System Water Cooled Chiller. Analytical Chemistry35(12), 1950-1953[Toilet Roll Tissue Wrapping Machine with Auto - Counting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Painting CanvasAuthor for correspondence