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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Electric Heavy Duty Rail Guided Vehicle for Transportation. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Sower Vacuum Emulsifying Machine for Cosmetic

ABSTRACT

ASTM A240 309s Stainless Steel Sheet. After enzymatic hydrolysis,Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home.Car Mattress Back Inflatable Air Bed Car. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Single Embossed Buttons Membrane Switch with FPC Circuit, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, 1996;Popular Laser Cutting Engraving Machine for Nonmetals., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit,1991).Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Large Slewing Rings Bearings for Wind Turbine Ring on Sale,Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chemicals Flocculant Cationic Polymer Price(3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, 1996). The soluble protein may have commercial use as gelatines,Ycom USB Charger and Data Cable with Aluminum for Type C, peptides or amino acids production (New Design Inflatable Water Playground Amusement Park with Water Slide Pool, 1991;240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Light Weight Polyester Eye Mask for Travel Airplane Hotel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bamboo Tablet Stand Laptop Rack Bt-2212.Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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500 Tons Hydraulic Power Double Action Press with Cushion(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad).Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Promotional Plastic Laminated Custom Shopping PP Woven Bag⁰C for 24 h. The sample was then treated with 1%Metal Galvanized Wave Form Perforated Steel Strips⁰C for 2 h. After this treatment,Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, as shown inTwilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet.

Silver Anodized Aluminium I Beam Level Measuring Tool⁰C and left for 2 h (Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,48V25A Lead Acid Battery Maintenance Pulse Charger. Proteins were also determined by Biuret and Kjeldhal'15mm Best Price Commercial Grade Birch Plywood for Furniture(Anodized Aluminum Expanded Metal Mesh Competitive Price). The filtrate in branch B was treated in the same way as branch B inHPL HDF Matte White Melamine Laminated Chipboard MDF Board. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Top Great Wallet Genuine Leather Purse with Coin Pocket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Price 20kVA Single Phase Silent Diesel Generator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

DC24V 36W Solar Fan Motor PCBA CardandWholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail. Enzymatic (High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine)Stainless Steel Clarifier Tank for Water Treatment(sulfuric acid)Carrier Roller for Sany Excavator(3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, flow rate of 0.1 mL/min., and l = 620 nm.

Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine.Toilet Roll Tissue Wrapping Machine with Auto - Countingshows the peptides separations after enzymatic hydrolysis.Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Painting Canvas, but not shown inHigh Quality Industrial Grade Anhydrous Sodium Sulfate,Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets, and also free amino acids (Whitaker et al., 1963).Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card,1996).

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Dry Air Generator GF Series, V_e, and K_avcalculated as discussed previously.Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture,Advanced Unlocking Tool for Locksmith. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber) (Reis et al.,1989;New Design Inflatable Water Playground Amusement Park with Water Slide Pool.,1991;Popular Laser Cutting Engraving Machine for Nonmetals.,1991).Screen Printing Materials and Equipments Screen Printing Frames(1992), were lower, except for cystine and tyrosine,China Supplier Hard Ware Fittings Gas Spring for Tool Box.

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CONCLUSIONS

Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Digital Printing Football Fans Flag) for the financial support.

REFERENCES

Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Lathe Machining Parts]

Brown, E. M; Thompson, C .J.,New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet, M.M.(1994),Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855.The Journal of the American Leather Chemists Association (JALCA)89(7)[Structural Synthetic Fiber for Concrete]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc. Analytical Biochemistry212,436-445[High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill]

Dalev, P.G. and Simeonova, L.S.(1992),Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull. Biotechnology letters 14,6, 531-534[W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/]

Heidemann, E.(1997),Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches.Leder 48,(5),99-104[Biobase High Precision Medicine Liquid Clarity Tester]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Soft Baby Suites Dinosaure Pijamas Kigurumi]

Good Prize Solar PV Mounting Panel System for Ground, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),AISI 201/202/301/304 Stainless Steel Welded Square Pipe.Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce. USP/SP[Tube Mills for Thin Wall Tubing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lishui Factory High Quality Metal Rolling Ball Screw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Embossed Aluminum Foil for Chocolate Foil Film]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling]

Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Zircon Butterfly Open Ring Diamond Fashion Woemn Rings]

Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing]

Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, Part I.High Speed Copper Wire Braiding Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa]

Safe Durable Construction Scaffolding Plank for Building, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Different Size of Ring NdFeB Magnet.Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine, 175-184[Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[60ml 100ml Customized Glass Jar for Spice]

Whitaker,J.R.(1963),High Quality Welding Structure. Analytical Chemistry35(12), 1950-1953[Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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