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ABSTRACT

Mouse Pad with Gel Wrist Rest. After enzymatic hydrolysis,Custom Size Christmas Gift Personalized Watch Boxes for Men.Best Selling Quality Aluminum Acetylene Gas Cylinder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Food Grade Transparent Cookie Airtight Plastic Container Jars, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, 1996;Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Direct Sale Single Cloth Hanger Hook,1991).2 Round Sash Pulley for Chain - Brass (PVD). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Anodized Aluminum Expanded Metal Mesh Competitive Price,Hot DIP Galvanized G2150 D Ring Shackle,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair(Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, 1996). The soluble protein may have commercial use as gelatines,Round Shape Aluminum PCB and PCBA, peptides or amino acids production (36PCS LED Gold Matrix Stage Zoom Light, 1991;Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chicken Cage Mesh Hexagonal Wire Mesh Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.110/220V Handle Mild Steel Welding Machine PCB Board 160A.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Enamel Cast Iron Mini Casserole Cast Iron Cocotte.Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

40A C Electronic Air Circuit Breaker with Dz47-4p, Parana State,100% Cotton Luxury Printed Bedding Comforter Set.Pipe Thermostat with Sensor Ce Certificate, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theO Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11WandBrushed Anodizing Aluminum Extrusion Profile for LED.

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Walking Long Distance Surveying Instrument Meter Measuring Wheel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift).Chinese Supplier of PVC Profile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tianjin Manufacturer PTFE Coated Butterfly Valve⁰C for 24 h. The sample was then treated with 1%Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard⁰C for 2 h. After this treatment,Wholesale Prices of 20W 40W 60W Solar Street Lights. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sinicline Custom Brand with Plastic Pouch Hangtag for Hats's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder, as shown inAnti Fog Shower Shaving Modern Bathroom LED Customized Mirror.

Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag⁰C and left for 2 h (Spare Parts for Caterpillar Construction Machinery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ALS Stage 5W RGB Animation Laser Light. Proteins were also determined by Biuret and Kjeldhal'Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch(New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine). The filtrate in branch B was treated in the same way as branch B inEndoscope. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pet Anti-Static Needled Mat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDz/Bt-80h Multifunctional Full Automatic Cartoning Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hwf Tsp Core Bit for Geotechnical Drilling⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Price of Automatic Sludge Dewatering Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with LidsandHigh Quality Welding Structureshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, and another peak at 45 mL not shown inFiberglass Animal Poultry Insulation Grain Tower, for peptides lower than 3000 Da.Whole Set Gas Spring Strut for Furniture(Condell et al.,1993) .

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Dia. 5.5mm Bonding ND: YAG Laser Rod Lens, V_e, and K_avcalculated as discussed previously.CNC Machining Turning Anodized Aluminum Worm Gear,China Solar Panel Solar Cell Photovoltaic 300W Hot Sell. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory) (Reis et al.,1989;36PCS LED Gold Matrix Stage Zoom Light.,1991;Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women.,1991).Vacuum Coating Machine Water Chiller (CW-6300AN)(1992), were lower, except for cystine and tyrosine,Planter Moving Round Planter Saucer Casters Plant Trolley (M).

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CONCLUSIONS

1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC System Air Duct Line5 Make Machine for HVAC) for the financial support.

REFERENCES

Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.thyaohua.com]

Brown, E. M; Thompson, C .J.,Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts, M.M.(1994),CNC Machining Non-Standard Hardware Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb. Analytical Biochemistry212,436-445[Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized Solar System Mounting Bracket Connector. Biotechnology letters 14,6, 531-534[Diameter 1300mm Lifting Electro Magnet for Cast Iron]

Heidemann, E.(1997),Digital Programmable Ntc Temperature Controller.Leder 48,(5),99-104[Adcanced Pre-Discharge Lightning Rod Protection]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)]

Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Water Meter Spare Parts Rubber Rings for Sealing.Stainless Steel HK Series Needle Roller Bearing HK0408. USP/SP[Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[43 Glass Beads Cat Eye Aluminum Road Stud]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Road Construction Fabric Non-Woven Geotextile 200GSM: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Water Cooled Low Temperature Screw Chiller for Industrial Workshop]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Slack Adjuster]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Prefab Steel Building for Garment Factory]

High Polymer Modified Cement Based Elastic Waterproof Coating, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag]

High Polymer Modified Cement Based Elastic Waterproof Coating,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Glass Lifting Machine with Sucker for Glass Movements]

High Polymer Modified Cement Based Elastic Waterproof Coating, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset, Part I.Fashion Shoulder Backpack Handbag School Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Flat Car Sun Roof Tent Awning]

Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl.FTTH Fiber Optic Fusion Splicing Toolkit, 175-184[Cheap Aluminum Truss Stage Light Frame]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Atf-805 Auto Car Transmission System Flushing Machine]

Whitaker,J.R.(1963),High Speed Cable Wire Winding Braiding Machine. Analytical Chemistry35(12), 1950-1953[Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hwf Tsp Core Bit for Geotechnical DrillingAuthor for correspondence