ARTICLES

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ABSTRACT

Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800. After enzymatic hydrolysis,The Most Competitive Sodium Gluconate for Water Reducer.Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electric Magic Hot Hair Styling Curling Iron with Brushes;Melt Pressure Transducer (PT133); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag, 1996;Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game,1991).Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord,Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Durable Aluminum Flag Pole with Dome Top Tangle Free Finial(Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag, 1996). The soluble protein may have commercial use as gelatines,Customized Marathon Medal for Marathon Medallion, peptides or amino acids production (Price of a Retail Supermarket Push Cart for Sale, 1991;Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lady Kids Child Yoga Clothes Gym Fitness Sports Wear., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hotel Steel Digital Room Safes with Large LED Display.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Biogas/Methane Purification System/Desulfurization/Scrubber Tower.Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Popular Custom School Uniform Woven Badge Patch with Merrow Border(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Flow Wrapper/ Bakery Moon Cake Packing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf).Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh⁰C for 24 h. The sample was then treated with 1%Reusable Metal Stainless Steel Drinking Straws⁰C for 2 h. After this treatment,Designed Steel Frame Factory Building. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table, as shown inLow Carbon Heavy Galvanized Steel Wire.

Scouring Pads Making Machine for Brush King⁰C and left for 2 h (50Hz to 60Hz Frequency Inverter for Water Pump). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Glass Mix Stone Mosaic Tiles. Proteins were also determined by Biuret and Kjeldhal'Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine(Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad). The filtrate in branch B was treated in the same way as branch B inSilicone Coated Heat Protective Hydraulic Hose Guard. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUsed/Second Hand 120mpm Continous Galvanizing Line/Cgi(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Racing Preformance PTFE Teflon Alloy Fuel Adapter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Stainless Steel Expanded Metal Mesh, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Big Chocolate Storage Holding Tank, Chocolate Buffer Tank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electrical Stationary Hoist Tower Crane 500kg Motor) for the financial support.

REFERENCES

Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Easy Application 1k Crystal Pearl Colors Car Paint]

Brown, E. M; Thompson, C .J.,Asco 353 Series 3/4 Pulse Valve Solenoid Valve, M.M.(1994),China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material.The Journal of the American Leather Chemists Association (JALCA)89(7)[Zircon Butterfly Open Ring Diamond Fashion Woemn Rings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle. Analytical Biochemistry212,436-445[Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa]

Dalev, P.G. and Simeonova, L.S.(1992),Purple UL 1095 16AWG Electronic Lead Wire. Biotechnology letters 14,6, 531-534[60ml 100ml Customized Glass Jar for Spice]

Heidemann, E.(1997),Brushed Anodizing Aluminum Extrusion Profile for LED.Leder 48,(5),99-104[Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent]

New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps.S235jo S235jr Steel Pipe Tube Shs Rhs. USP/SP[Humidity Refrigeration Digital Controller Jsd-100+]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nylon Weatherproof PA6 Strain Relief Cable Gland]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Galvanized Steel Oval Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Bright Polished Mirror Sheet]

Pre-Seasoned Cast Iron Mini Oval Casserole Pot, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Square Wire Mesh Netting with SGS-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core]

Pre-Seasoned Cast Iron Mini Oval Casserole Pot,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electrical Barber Shop Equipment Electric Hair Curling Wand Iron]

Pre-Seasoned Cast Iron Mini Oval Casserole Pot, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, Part I.Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[HDPE Polyethylene PE Pipe for Water Supply]

Outdoor Garden Save Energy with Solar Flame Wall Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag, 175-184[Anti Metal Rfid Tag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chinese Supplier of PVC Profile]

Whitaker,J.R.(1963),Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length. Analytical Chemistry35(12), 1950-1953[Non Rechargeable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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