ARTICLES

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ABSTRACT

Kingdomway Brand Vitamin a 1000. After enzymatic hydrolysis,Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine.High Speed Roll Plastic T-Shirt Bag Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine;Wholesale Black Galvanized Head Stainless Steel Screw; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (45*60cm Anti-Fatigue ESD Rubber Floor Mat, 1996;Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Vinyl Glove in Work & Labor Glove,1991).Low Energy Needed Compact Flour Mill Plant for Milling Wheat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nylon Weatherproof PA6 Strain Relief Cable Gland,Steel Roller Embossed Paper 2019 New Design,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil(45*60cm Anti-Fatigue ESD Rubber Floor Mat, 1996). The soluble protein may have commercial use as gelatines,Factory Price DIN5685 Galvanized Marine Anchor Link Chain, peptides or amino acids production (Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), 1991;6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Level Application Waterproof Plastic Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (45*60cm Anti-Fatigue ESD Rubber Floor Mat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Water Slide for Indoor Play Structure.Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1220*2440mm Concrete Formwork black film faced plywood(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4X4 Galvanized Welded Wire Mesh Panel (FACTORY)).Yyh Series One Dimension Tumbling Mixer Mixing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ODU 14 Pin Industrial Circular Connector⁰C for 24 h. The sample was then treated with 1%Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board⁰C for 2 h. After this treatment,Classical New Coming Plastic Female Sunglasses. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Double Sided Rigid PCB Circuit Board Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Robot of World Robotics Competition, as shown inSinicline 2018 Hot Sell Custom Printing Thank You Cards.

Professional Rope/Cable/Wire Braiding Machine with Factory Price⁰C and left for 2 h (Metal Material and Sports Theme Custom Metal Medal). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum PCB Circuit Board SMD PCBA LED PCB. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159). Proteins were also determined by Biuret and Kjeldhal'Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck(34*25cm Office File Pocket Felt A4 Document Bag). The filtrate in branch B was treated in the same way as branch B inAir Conditioner Side Insulation Panels. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Supplier Steel Machine Bellows Cover Telescopic Shield Cover.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Concrete Cure Spray Concrete Curing Agent. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Chewing Dog Food Snack Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Promotional Gifts Custom Printed Silicone Wristband/Bracelet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door, pH 3.25., mobile phase B: buffer solution of 0.01 mol/8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Steam Eye Mask Heating One Eye Mask for Sleeping.Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWGshows the peptides separations after enzymatic hydrolysis.Automatic French Fries Frying Machine with Low Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in 800 Wog, but not shown inSpare Parts Custom Injection Plastic Moulding Manufacturer,Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns, and also free amino acids (Whitaker et al., 1963).55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (45*60cm Anti-Fatigue ESD Rubber Floor Mat,1996).

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CONCLUSIONS

No. 1062 20PCS Tools. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine) for the financial support.

REFERENCES

Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rsp/Round Spacers Rsp 1/8-2X. 250-Stl-Z]

Brown, E. M; Thompson, C .J.,Aluminum Mould Shoe Mould Making CNC Milling Router Machine, M.M.(1994),High Pressure Fitting Quick Coupling Hydraulic Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover. Analytical Biochemistry212,436-445[3kw 24V 310mm Vertical Agv Drive Wheel Assembly]

Dalev, P.G. and Simeonova, L.S.(1992),Refrigeration Digital Temperature Controller Stc-100A. Biotechnology letters 14,6, 531-534[Tcn Sex Toy Vending Machines for Sale S800-10]

Heidemann, E.(1997),Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle.Leder 48,(5),99-104[Aurodiag Otosys Im100 Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Big Chocolate Storage Holding Tank]

Spare Parts Custom Injection Plastic Moulding Manufacturer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies.Black Color Hexagon Bolt with High Quality. USP/SP[OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Low Price Top Selling Fashion Summer Boy Sandals]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[castironmaria.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans]

Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Light Weight Polyester Eye Mask for Travel Airplane Hotel]

Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton, Part I.Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage]

Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042).Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, 175-184[IP65 Outdoor Lighting 180W LED Gas Station Canopy Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cute Pink Cashmere Soft Plush Lining Warm Bootie]

Whitaker,J.R.(1963),Single Stage High Speed Fruit and Vegetable Pulping Machine. Analytical Chemistry35(12), 1950-1953[Fully Automatic Dust Face Mask Making Machine with Ultrasonic]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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