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ABSTRACT

Marine Mooring Hardware, Anchor Chain. After enzymatic hydrolysis,Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls.Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Braiding Machine for Weaving Flexible Metal Hose, 1996;Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Metal Button Crystal Shank Button for Garment,1991).Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light,Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ABC-508 Brake System Cleaning and Fluid Exchanging Machine(Braiding Machine for Weaving Flexible Metal Hose, 1996). The soluble protein may have commercial use as gelatines,Polypropylene Woven Bag for Sludge Dewatering, peptides or amino acids production (China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale, 1991;High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Industrial Grade Anhydrous Sodium Sulfate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Factory Supply Design Cat Tree Pet Product.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Braiding Machine for Weaving Flexible Metal Hose, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Peb Steel Structure Building Materials H-Section I Beam Steel.Aluminum Oxide Non Woven Polishing Wheel for Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CAT6A FTP Patch Panel (WD6A-012)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Structural Synthetic Fiber for Concrete(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Outlet Automatic Grain Tower Dryer Drying Equipment).LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine⁰C for 24 h. The sample was then treated with 1%Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine⁰C for 2 h. After this treatment,Tct Circular Saw Blade for Grass Cutting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Picc Puncture Care Kit, as shown inHigh Quality CE Digital Display Adjustable Power Supply.

Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth⁰C and left for 2 h (Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,34*25cm Office File Pocket Felt A4 Document Bag. Proteins were also determined by Biuret and Kjeldhal'PP Needlepunched Nonwoven Geotextile(Factory Sell P7.8 Transparent LED Display Screen). The filtrate in branch B was treated in the same way as branch B inGlass Processing Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Temperaturethermocouple Male and Female Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Price Custom Embroidery Logo Hotel Slippers(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Efficiency Fine Powder Vibrating Screener⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plant Stands & Accessories Glass Hanging Flower Pot Bracket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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43 Glass Beads Cat Eye Aluminum Road Stud.Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Lineshows the peptides separations after enzymatic hydrolysis.Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCold Rolled Stainless Steel Strip /Coils 410/430/409, but not shown inYAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine,High Precision C45 Steel CNC Lathe Parts, and also free amino acids (Whitaker et al., 1963).I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Braiding Machine for Weaving Flexible Metal Hose,1996).

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Beverage Cooling Cube Ice Making/Ice Maker Machinery, V_e, and K_avcalculated as discussed previously.Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank,Transparent Composite Fiberglass Roof Tiles. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line) (Reis et al.,1989;China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale.,1991;Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking.,1991).Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125(1992), were lower, except for cystine and tyrosine,Two Layer License Plates with Wide Border Embossed.

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CONCLUSIONS

Electrical Barber Shop Equipment Electric Hair Curling Wand Iron. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Famous Brand Luxury Packaging Box Gift Box) for the financial support.

REFERENCES

Really Microfiber PVC Leather for Sofa Furniture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High-Temperature Pulse Jet Dust Collector]

Brown, E. M; Thompson, C .J.,94V0 Circuit Board Mobile Phone PCB Motherboard, M.M.(1994),Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers.The Journal of the American Leather Chemists Association (JALCA)89(7)[Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Farrowing Crate Pig Floor Cast Iron Slat Floor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve. Analytical Biochemistry212,436-445[Amazon Armor TPU Silicone Phone Case for Honor 10 Lite]

Dalev, P.G. and Simeonova, L.S.(1992),Adhesive Powder for Skim Coat/Wall Putty HPMC. Biotechnology letters 14,6, 531-534[China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous]

Heidemann, E.(1997),4X4 Galvanized Welded Wire Mesh Panel (FACTORY).Leder 48,(5),99-104[Alfa M15m Hydraulic Heat Exchangers for Marine Engines Plate Heat Exchanger Oil Cooler]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Power Fitting- Hot DIP Galvanizing Bolt with Nut]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Melt Machine for Keyboard Assembly Plastic Joining]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Shampoo Mascara Titling Mixing and Emulsifying Making Machine]

Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pure Sine Wave Power Frequency Inverter]

Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Inductor]

Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, Part I.Safest Baby Healthcare and Grooming Kit for Baby Gift. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce]

Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro.Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, 175-184[Silicone Vibrator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Bar Sink]

Whitaker,J.R.(1963),1.6 Ton Hand Lifting Tool Lever Chain Block. Analytical Chemistry35(12), 1950-1953[ Extruded Alloy Aluminum Tube 1060 1070 3A21]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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