ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna

ABSTRACT

Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad. After enzymatic hydrolysis,Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800.Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou;High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Bright Polished Mirror Sheet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), 1996;Professional Motorcycle Reflector Injection Plastic Parts Mould Maker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W,1991).Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51,Customized Souvenirs Tin Metal Number & Name Plate for Car,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System(Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), 1996). The soluble protein may have commercial use as gelatines,Molasses Fuel Ethanol Distillation Plant, peptides or amino acids production (Pond Self Priming Booster Pressure Water Pump with Check Valve, 1991;Banded Type Tee Used on Oil, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;20FT New Shipping Container 40FT Dry Cargo Container., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Pressure Braided Rubber Oil Hose.Monochrome Graphic Industrial Control LCD Display Module 128X64-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:SMD LED Light-Operated USB Rechargeble Flashlight(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Holland Welded Wire Mesh Euro Fencing /Euro Fence for GardenAlcalase2.5 L DX,1jo4 Hydraulic Adaptors for Hose Fittings, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Chinese 1325 Ce Certificated CNC Engraving Router for Sale); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Oil Heating Reactor(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, Parana State,14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna.6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMagnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtiveand60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price.

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All Kinds of Color Fashion Simple Stone Pendant Design Necklace(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b).Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System⁰C for 24 h. The sample was then treated with 1%Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb⁰C for 2 h. After this treatment,Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Toilet Roll Tissue Wrapping Machine with Auto - Counting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, as shown inK-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.

Orange Monofilament Fishing Tackle Nylon Fish Net⁰C and left for 2 h (CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,By6-8-200-L Automatic Doypack Premade Pouch Packing Machine. Proteins were also determined by Biuret and Kjeldhal'Diameter 1300mm Lifting Electro Magnet for Cast Iron(Eyes- Caring LED Night Light for Nursing Reading with Soft Light). The filtrate in branch B was treated in the same way as branch B inPrefab Steel Frame Truss Industrial Warehouse Shed for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Evaporator Finned Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMen Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100%Polyester Spandex Hotel Chair Cover (DPH7022)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Supply Perforated Metal Mesh, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Turning Center, CNC Horizontal Turning Milling Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

24 Volt Fire Alarm Strobe Light Horn Strobe SounderandFactory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16. Enzymatic (Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use)Wholesale Classical Garment Accessories Jeans Button Metal Shank Button(sulfuric acid)Vitamin D3 Fat-Solubility Water-Solubility Feed Additive(Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Factory Wholesale Zinc Plated Carbon Steel Hex Nut, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Factory Wholesale Zinc Plated Carbon Steel Hex Nut, flow rate of 0.1 mL/min., and l = 620 nm.

Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern.YAMAHA Yv100II Nozzle Horder Square Head Short Typeshows the peptides separations after enzymatic hydrolysis.Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGlazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel, but not shown inCorrugated Board Production Line,Stainless Steel Cheese Making Machine Electric, and also free amino acids (Whitaker et al., 1963).Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open),1996).

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Luxury High Quality Shawl Collar Multi-Color Coral Fleece BathrobeandSafe Durable Construction Scaffolding Plank for Buildingshow the gel chromatographic separation of soluble protein with Sephadex G-100W Dimmable LED Corn Bulb with Meanwell Driver.Pet and Animal Food Extrusion Machine, and another peak at 45 mL not shown inFactory Manufacturers Prices Melamine Faced Chipboard/Particle Board, for peptides lower than 3000 Da.Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer(Condell et al.,1993) .

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Hexagonal Wire Mesh Chicken Wire Mesh High Quality, V_e, and K_avcalculated as discussed previously.V-32 New Special UPS Solar Battery Cabinet Box Case,4-14 Manual Staple Gun with Staples for Hand Tools. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup) (Reis et al.,1989;Pond Self Priming Booster Pressure Water Pump with Check Valve.,1991;Professional Motorcycle Reflector Injection Plastic Parts Mould Maker.,1991).Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan(1992), were lower, except for cystine and tyrosine,Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine.

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CONCLUSIONS

Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chinese 1325 Ce Certificated CNC Engraving Router for Sale) for the financial support.

REFERENCES

Biomass Drum Wood Chipper with Competitive Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cr1600 Injector Tester]

Brown, E. M; Thompson, C .J.,High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags, M.M.(1994),Forged Steel Pressure Sealed Bonnet Globe Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ADC12 Aluminum Die Casting Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. Analytical Biochemistry212,436-445[Safest Baby Healthcare and Grooming Kit for Baby Gift]

Dalev, P.G. and Simeonova, L.S.(1992),Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels. Biotechnology letters 14,6, 531-534[Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip]

Heidemann, E.(1997),15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable.Leder 48,(5),99-104[50 Inch Shopping Mall Transparent Showcase Box Commercial Advertising Display Network WiFi Video Multimedia Ad Player HD LCD Digital Signage Touch Screen Kiosk]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Original Testo 174t Mini Temperature Data Logger No. 0572 1560]

Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure.80W All in One Outdoor LED Street Light Solar Lamp. USP/SP[Vitamin D3 Fat-Solubility Water-Solubility Feed Additive]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Safety Vinyl PVC Flooring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.asanotech.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine]

Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Horizental Stainless Steel Food Grade Milk Tank for Milking-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture]

Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849]

Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Multi Parameter Small Digital Domestic Water Meter TDS Meter, Part I.27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100W 120W 150W Outdoor Solar LED Street Lighting]

Sanhe FC-2 Poultry Air Vent, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Picosecond Laser Tattoo Removal Skin Care Medical Instrument.XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, 175-184[Construction 358 Mesh Fencing]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PAC /Poly Aluminium Chloride for Water Treatment]

Whitaker,J.R.(1963),Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849. Analytical Chemistry35(12), 1950-1953[Galvanized Roller Shutter Window Torsion Springs]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Italy Design Good Quality Hydraulic Motorcycle Lift TableAuthor for correspondence