ARTICLES

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^IICNPq/RHAE/biotechnology-500t Plywood Hot Press Woodworking Machine (12-25 layers). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,10ml Round Clear Nail Polish Bottle with Brush Cap

ABSTRACT

Full Automatic 4000b/h 500ml Plastic Jar Making Machine. After enzymatic hydrolysis,Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine.5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Laundry Used Gas Water Heater with CE/Asme Certificate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (15W Aquarium Submersible UV Lamp Durable Germicidal Lamp, 1996;2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Accept OEM New Products Team Various Colors Gold Ribbon,1991).Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh,24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair(15W Aquarium Submersible UV Lamp Durable Germicidal Lamp, 1996). The soluble protein may have commercial use as gelatines,What′s Kind of Machine for Making Organic Fertilizer Granules, peptides or amino acids production (Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, 1991;Home and Hospital Electric Patient Lift, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (15W Aquarium Submersible UV Lamp Durable Germicidal Lamp, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DC 12V Power Relay for Smart House.2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Medical Human Body Thermometer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table).Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Supplier Granular Coal Based Activated Carbon Filter Meida⁰C for 24 h. The sample was then treated with 1%Glass Cutting Table/CNC Glass Cutting Machine⁰C for 2 h. After this treatment,12 Bottles Portable Home Ues Thermoelectric Wine Chiller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), as shown inChampagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net.

Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap⁰C and left for 2 h (Two Layer License Plates with Wide Border Embossed). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle. Proteins were also determined by Biuret and Kjeldhal'Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe(Horizental Stainless Steel Food Grade Milk Tank for Milking). The filtrate in branch B was treated in the same way as branch B inSt Fiber Optic Connector Cable Adapter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQ35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hanwha SMT Chip Shooter LED Pick and Place Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ACP Aluminium Cmposite Panel Cladding Extrusions, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control.Wholesale Classical Garment Accessories Jeans Button Metal Shank Buttonshows the peptides separations after enzymatic hydrolysis.Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEtching Rear Trunk Sill Plates for Hyundai Tucson, but not shown inSurgical Power Tool Medical Care Orthopedic Mini Bone Saw,Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, and also free amino acids (Whitaker et al., 1963).Marine Mooring Hardware, Anchor Chain. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (15W Aquarium Submersible UV Lamp Durable Germicidal Lamp,1996).

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CONCLUSIONS

Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products) for the financial support.

REFERENCES

10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mini Mason Jar Shot Glass]

Brown, E. M; Thompson, C .J.,Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, M.M.(1994),ABC-508 Brake System Cleaning and Fluid Exchanging Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Aluminum Heat Sink for Electronics Computer Electric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polypropylene Woven Bag for Sludge Dewatering. Analytical Biochemistry212,436-445[Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale]

Dalev, P.G. and Simeonova, L.S.(1992),China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale. Biotechnology letters 14,6, 531-534[Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag]

Heidemann, E.(1997),High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing.Leder 48,(5),99-104[Pivot Door Sliding Hinge]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset]

Powerful Cleaning Colorful Scouring Pad for Kitchen Use, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Industrial Grade Anhydrous Sodium Sulfate.Professional Factory Supply Design Cat Tree Pet Product. USP/SP[Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Powder Coated Expanded Mesh]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Snsc Fd30 Side Shift New Diesel Forklift 3ton]

Peb Steel Structure Building Materials H-Section I Beam Steel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Oxide Non Woven Polishing Wheel for Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Glass Mix Stone Mosaic Tiles]

Peb Steel Structure Building Materials H-Section I Beam Steel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Palet Coil Nail Welding Machine/Nail Making Machine]

Peb Steel Structure Building Materials H-Section I Beam Steel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CAT6A FTP Patch Panel (WD6A-012), Part I.LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby]

Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Famous Brand Luxury Packaging Box Gift Box.High Qualified Medical Grade Thin Wall Stainless Steel Tube, 175-184[Black Powder Dispersed Plugging Agent]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Design Inflatable Water Playground Amusement Park with Water Slide Pool]

Whitaker,J.R.(1963),3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry. Analytical Chemistry35(12), 1950-1953[Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Car Badge Custom Emblem Auto Logo Sign for AdvertisingAuthor for correspondence