ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Cold Rolled Stainless Steel Strip /Coils 410/430/409. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,FRP Water Tank GRP Fishing Tanks From Factory Supply

ABSTRACT

Decorative House Stone Coated Metal Roof Tile. After enzymatic hydrolysis,Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig.6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Blue Steel Packing Strip, 1996;High Quality Powder and Wax Brush with Wooden Handle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheap Price Paper Straws Individually Wrapped Drinking Straw,1991).ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF,Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Smelting and Heating Induction Machine (GW-HY187)(Blue Steel Packing Strip, 1996). The soluble protein may have commercial use as gelatines,Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem, peptides or amino acids production (Home Products 16′ Perforated Flex Pipe, 1991;1220 Fork Length 3-Wheel Electric Forklift Truck, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.FM USB Radio Audio Power Amplifier for Home Bar Club.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Blue Steel Packing Strip, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sell Mechanical Bar Screen for Water Treatment.Glow Powder for Luminous Coating-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:700 mm RP HP UHP Graphite Electrode Sold at Factory Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fused Quartz Powder 325mesh 500mesh 1250mesh. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avVarious Types of Plastic Hooks Mould From Manufacturers(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller) for the financial support.

REFERENCES

Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Lengthening Drilling Diamond Three-Piece Core Drill/Diamond Tool]

Brown, E. M; Thompson, C .J.,W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/, M.M.(1994),Cake/Cookies/Bread Automatic Package and Packing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reusable Metal Stainless Steel Drinking Straws]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customize Milky Extrusion PC Cover. Analytical Biochemistry212,436-445[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Dalev, P.G. and Simeonova, L.S.(1992),European Good Quality Bathroom Simple Sliding Glass Shower Cabina. Biotechnology letters 14,6, 531-534[ D1400]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-11.1V 2000mAh Lithium Polymer Battery Pack Customized Size: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Hv / Coating Material Grade CMC Medium Viscosity]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alumium Rotary Oil Fuel Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Efficiency Fine Powder Vibrating Screener]

Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Jh21 Series Pneumatic Power Press CNC Punching Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules]

Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Double Roller Rotary Wood Peeling Machine with CE for Sale]

Whitaker,J.R.(1963),Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production. Analytical Chemistry35(12), 1950-1953[Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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