ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Whole Set Gas Spring Strut for Furniture. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Dia. 5.5mm Bonding ND: YAG Laser Rod Lens

ABSTRACT

CNC Machining Turning Anodized Aluminum Worm Gear. After enzymatic hydrolysis,China Solar Panel Solar Cell Photovoltaic 300W Hot Sell.Vacuum Coating Machine Water Chiller (CW-6300AN). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Planter Moving Round Planter Saucer Casters Plant Trolley (M);1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts, 1996;CNC Machining Non-Standard Hardware Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb,1991).Galvanized Solar System Mounting Bracket Connector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Digital Programmable Ntc Temperature Controller,Water Meter Spare Parts Rubber Rings for Sealing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel HK Series Needle Roller Bearing HK0408(Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts, 1996). The soluble protein may have commercial use as gelatines,Road Construction Fabric Non-Woven Geotextile 200GSM, peptides or amino acids production (High Polymer Modified Cement Based Elastic Waterproof Coating, 1991;Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Shoulder Backpack Handbag School Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl.FTTH Fiber Optic Fusion Splicing Toolkit-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Speed Cable Wire Winding Braiding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll MillAlcalase2.5 L DX,High Temperature Alternative Stainless Steel Band in Different Package, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Water Cooled Low Temperature Screw Chiller for Industrial Workshop); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Home Machine 2019 New Commercial Treadmill Gym Equipment(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card, Parana State,Dia. 5.5mm Bonding ND: YAG Laser Rod Lens.Residential Reverse Osmosis 0.0001um Water Filter Water Filtration, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCar Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan AltimaandRoller Wedge Screen Cylinder.

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Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women).China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Folding Round Picnic Caravan Table RV Picnic Table Camping Table⁰C for 24 h. The sample was then treated with 1%Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits⁰C for 2 h. After this treatment,3HP Industrial Cooling System Water Cooled Chiller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wood Veneer Peeling and Clipping Combined Machine, as shown inElbow Forming Machine Gorelocker HVAC Duct Making Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Corrugated Board Production Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMagnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Engieered Veneer Faced Commericial Plywood⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hex Thin Head Nut, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Nuts

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CONCLUSIONS

ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Water Cooled Low Temperature Screw Chiller for Industrial Workshop) for the financial support.

REFERENCES

Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Goods Lift Manufacturer]

Brown, E. M; Thompson, C .J.,Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose, M.M.(1994),Smelting and Heating Induction Machine (GW-HY187).The Journal of the American Leather Chemists Association (JALCA)89(7)[China Customized Chilled Cast Iron Roll]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Water Slide for Indoor Play Structure]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem. Analytical Biochemistry212,436-445[PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate]

Dalev, P.G. and Simeonova, L.S.(1992),Home Products 16′ Perforated Flex Pipe. Biotechnology letters 14,6, 531-534[Tct Circular Saw Blade for Grass Cutting]

Heidemann, E.(1997),1220 Fork Length 3-Wheel Electric Forklift Truck.Leder 48,(5),99-104[Abs Wing Nut Hose Clamp]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Gold Marathon Running Sport Medal for Promotion Gift]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbide Engraving Tools Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator]

Hot Sell Mechanical Bar Screen for Water Treatment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Glow Powder for Luminous Coating-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management]

Hot Sell Mechanical Bar Screen for Water Treatment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mesh Bag Packing 20kg Pure White Garlic]

Hot Sell Mechanical Bar Screen for Water Treatment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:700 mm RP HP UHP Graphite Electrode Sold at Factory Price, Part I.Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet]

Union Metal Steel Building and Construction Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller.450ml Sublimation Stainless Steel Milk Bottle (Colorful), 175-184[Jis 42mm Stainless Steel Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[304 Stainless Steel Anti-Corrosion Welded Wire Mesh]

Whitaker,J.R.(1963),Lower Noise Colled Rolled Ball Screw. Analytical Chemistry35(12), 1950-1953[Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Electric Heavy Duty Rail Guided Vehicle for TransportationAuthor for correspondence