ARTICLES

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ABSTRACT

AISI 201/202/301/304 Stainless Steel Welded Square Pipe. After enzymatic hydrolysis,Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce.Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger;PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Speed Copper Wire Braiding Making Machine, 1996;Safe Durable Construction Scaffolding Plank for Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Different Size of Ring NdFeB Magnet,1991).Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Welding Structure,Carboxymethyl Cellulose CAS No. 9004-32-4,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher(High Speed Copper Wire Braiding Making Machine, 1996). The soluble protein may have commercial use as gelatines,Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip, peptides or amino acids production (150kg Per Hour Frozen French Fries Production Line, 1991;Automatic Professional LED Display Ceramic Hair Curling Iron, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Vulcanizing Machine /Rubber Tiles Vulcanzing Press. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Speed Copper Wire Braiding Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low.Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Omni Newest Manager Desk Office Smart Office Table Office Staff Table(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Various Types of Plastic Hooks Mould From Manufacturers).Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%As568 Standard Brown FKM Rubber O Ring for Sealing⁰C for 24 h. The sample was then treated with 1%The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent⁰C for 2 h. After this treatment,Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)200W UFO LED High Bay Light Fixture for Interior Exterior Applications's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2), as shown inUnisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Borosilicate Glass Baking Dish Set Clear. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avG08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Colorful  Reflective Sticker Reflective Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage) for the financial support.

REFERENCES

Disposable PS Foam Fast Food Box Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Reach Driver Storage Travel Hanging Car Seat Organizer (AKB084)]

Brown, E. M; Thompson, C .J.,Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), M.M.(1994),Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP.The Journal of the American Leather Chemists Association (JALCA)89(7)[Promotional Gifts Custom Printed Silicone Wristband/Bracelet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Foska Good Quality Plastic Stapler Set with Staples. Analytical Biochemistry212,436-445[High Quality Stainless Steel Plate Flange]

Dalev, P.G. and Simeonova, L.S.(1992),Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck. Biotechnology letters 14,6, 531-534[Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)]

Heidemann, E.(1997),Hotel SPA Swimming Pool, Sport Room, Bathroom Towel.Leder 48,(5),99-104[Rotary Hydraulic Cylinder Rotary Actuator Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Supplier V Wedge Wire Johnson Water Well Screen]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10ml Round Clear Nail Polish Bottle with Brush Cap: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[8mm Reflective Heat Strengthed Ceramic Firt Glass Panels]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Paper Sticky Lint Remover Roller/Pet Hair Roller]

Full Automatic 4000b/h 500ml Plastic Jar Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen]

Full Automatic 4000b/h 500ml Plastic Jar Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets]

Full Automatic 4000b/h 500ml Plastic Jar Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine, Part I.PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Colorful 100% Cotton Hotel Face Towels]

Hermetic Sliding Lead Door, X Ray Room Door, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Laundry Used Gas Water Heater with CE/Asme Certificate.15W Aquarium Submersible UV Lamp Durable Germicidal Lamp, 175-184[Stainless Steel Square Pipe (304L 321 316)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches]

Whitaker,J.R.(1963),2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b. Analytical Chemistry35(12), 1950-1953[Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building MaterialAuthor for correspondence