ARTICLES

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ABSTRACT

Multi Color Spade Made of Steel and Panting. After enzymatic hydrolysis,Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality.Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Condition Hot Sale Type Air Cooled Ice Cube Maker;Stainless Steel Hinge Pin, Locking Hinge Pin; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4 Way Stretch Running Gym Shorts Unique Women Short Pants, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (VHDCI Connector, 100p, Solder 0.8mm Pitch, 1996;6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Must 4kw 2HP Pump Power Inverter Ags Bts Available,1991).K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling,Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100W 120W 150W Outdoor Solar LED Street Lighting(VHDCI Connector, 100p, Solder 0.8mm Pitch, 1996). The soluble protein may have commercial use as gelatines,Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), peptides or amino acids production (Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody, 1991;Economic Full Automated SMT Production Line PCB Production Machinery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (VHDCI Connector, 100p, Solder 0.8mm Pitch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600.HDPE Geomembrane Lining Price for Pond Liner Film-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

IMTEK High Precision and Rigidity Linear Guides for CNC machinery, Parana State,Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover.Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (VHDCI Connector, 100p, Solder 0.8mm Pitch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent TrapsandMagic Hair Twist   Curl Sponge Brush.

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Yashi Touch Screen Smart Interactive Whiteboard for Office(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter).Electric Fireplace PCBA with Double Layers Circuit Board(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Shipping Logistics to Stockholm⁰C for 24 h. The sample was then treated with 1%Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting⁰C for 2 h. After this treatment,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Animal Agricultural Damping Horse Stall Rubber Cow Mat, as shown inAsco 353 Series 3/4 Pulse Valve Solenoid Valve.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom-Made Cotton Satin Towels for Adults with Thick Bath Towel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100% Cotton Luxury Printed Bedding Comforter Set⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mouse Pad with Gel Wrist Rest, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Size Christmas Gift Personalized Watch Boxes for Men, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Funny Newest Design for Children Theme Outdoor Playground ParkandBest Selling Quality Aluminum Acetylene Gas Cylinder. Enzymatic (Coal Mine Mini Electric Excavator with Quick Coupling)2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank(sulfuric acid)Food Grade Transparent Cookie Airtight Plastic Container Jars(VHDCI Connector, 100p, Solder 0.8mm Pitch, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, flow rate of 0.1 mL/min., and l = 620 nm.

Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women.Braiding Machine for Weaving Flexible Metal Hoseshows the peptides separations after enzymatic hydrolysis.Factory Direct Sale Single Cloth Hanger Hook(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Nc Studio Control, but not shown inProfessional Manufacture Electromagnetic Impulse Solenoid Valve,2 Round Sash Pulley for Chain - Brass (PVD), and also free amino acids (Whitaker et al., 1963).Anodized Aluminum Expanded Metal Mesh Competitive Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (VHDCI Connector, 100p, Solder 0.8mm Pitch,1996).

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36PCS LED Gold Matrix Stage Zoom Light, V_e, and K_avcalculated as discussed previously.Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China,Chicken Cage Mesh Hexagonal Wire Mesh Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa) (Reis et al.,1989;Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody.,1991;6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube.,1991).110/220V Handle Mild Steel Welding Machine PCB Board 160A(1992), were lower, except for cystine and tyrosine,Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming.

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CONCLUSIONS

Enamel Cast Iron Mini Casserole Cast Iron Cocotte. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chinese Galvanized Rectangular Tube for Buildings Materials) for the financial support.

REFERENCES

Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[9 - 9v Battery Clip]

Brown, E. M; Thompson, C .J.,Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer, M.M.(1994),Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Commercial Use Click Lock Standard Spc Vinyl Plank Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine. Analytical Biochemistry212,436-445[Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler]

Dalev, P.G. and Simeonova, L.S.(1992),CNC System Air Duct Line5 Make Machine for HVAC. Biotechnology letters 14,6, 531-534[Aluminum Adjustable Quick Assemble Stage with Steps]

Heidemann, E.(1997),Titanium Dioxide Anatase for Paper Making.Leder 48,(5),99-104[150kw High Power Electric Vehicle DC EV Connector GB/T 20234 Standard]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Control Housing with Plate Shape (XD-CC04)]

Stainless Steel Metric Female Flat Seat Hose Fittings, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),40A C Electronic Air Circuit Breaker with Dz47-4p.Pipe Thermostat with Sensor Ce Certificate. USP/SP[ DIN914]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Car Air Conditional Compressor Magetic Clutch 5pk]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Walking Long Distance Surveying Instrument Meter Measuring Wheel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[45*60cm Anti-Fatigue ESD Rubber Floor Mat]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[18inch Rectangle Melamine Polka DOT Tray]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys]

Chinese Supplier of PVC Profile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tianjin Manufacturer PTFE Coated Butterfly Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale 100% Polyester Cartoon Kids Sleepwear]

Chinese Supplier of PVC Profile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[15mm Best Price Commercial Grade Birch Plywood for Furniture]

Chinese Supplier of PVC Profile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard, Part I.Wholesale Prices of 20W 40W 60W Solar Street Lights. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine]

Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder.Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag, 175-184[Scaffolding Galvanized Steel Planks/Metal Deck for Construction]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility]

Whitaker,J.R.(1963),VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A). Analytical Chemistry35(12), 1950-1953[201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ASTM A240 309s Stainless Steel SheetAuthor for correspondence