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ABSTRACT

Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh. After enzymatic hydrolysis,24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light.Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;What′s Kind of Machine for Making Organic Fertilizer Granules;Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Home and Hospital Electric Patient Lift, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, 1996;M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts,1991).DC 12V Power Relay for Smart House. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment,Medical Human Body Thermometer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.(Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, 1996). The soluble protein may have commercial use as gelatines,Greenhouse Fixing Film Profile Lock Channel and Spring Wire, peptides or amino acids production (30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, 1991;Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52.China Supplier Granular Coal Based Activated Carbon Filter Meida-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glass Cutting Table/CNC Glass Cutting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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ACP Aluminium Cmposite Panel Cladding Extrusions⁰C and left for 2 h (Flour Making Machine Wheat Maize Flour Mill Machinery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Custom Mens Heated Down Jacket. Proteins were also determined by Biuret and Kjeldhal'Rectification Triming Metallized Polyester Film Slitting Rewinding Machine(Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns). The filtrate in branch B was treated in the same way as branch B inAutomatic Laser Cutter Steel for Sheet Metal/Cabinet/Signs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Swim Shorts Grey Mens Board Shorts with Mesh Liner. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMultifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Marine Mooring Hardware, Anchor Chain, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking.Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machineryshows the peptides separations after enzymatic hydrolysis.Fashion Metal Button Crystal Shank Button for Garment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inExplosion-Proof Housing Sealing Dispensing Machine, but not shown inEasy Operational Cup Cake Filling Machine,Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), and also free amino acids (Whitaker et al., 1963).10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles,1996).

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CONCLUSIONS

Peb Steel Structure Building Materials H-Section I Beam Steel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)) for the financial support.

REFERENCES

Aluminum Oxide Non Woven Polishing Wheel for Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[20w Fiber Laser Marking Machine]

Brown, E. M; Thompson, C .J.,CAT6A FTP Patch Panel (WD6A-012), M.M.(1994),LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Machining Service High Precision CNC Mechanical Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant. Analytical Biochemistry212,436-445[500t Plywood Hot Press Woodworking Machine (12-25 layers)]

Dalev, P.G. and Simeonova, L.S.(1992),Famous Brand Luxury Packaging Box Gift Box. Biotechnology letters 14,6, 531-534[Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks]

Heidemann, E.(1997),High Qualified Medical Grade Thin Wall Stainless Steel Tube.Leder 48,(5),99-104[Autostereoscopic 3d Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Precision Customized OEM Auto Hardware Lathe CNC Machining Parts]

2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry.High Strength New 0.18mm Molybdenum Wire. USP/SP[Blue Steel Packing Strip]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Structural Synthetic Fiber for Concrete: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[10A MPPT Solar Controllers PV 60V for off-Grid]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Liquid Silicone Rubber Shoe Flowers Moulding Micro Soft Plastic PVC Injection Molding Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FTTH Fiber Optic Fusion Splicing Toolkit]

LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Safe Durable Scaffolding Trapdoor Plank for Construction]

LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Supplied High Strength Marine Anchor Chain]

LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine, Part I.Tct Circular Saw Blade for Grass Cutting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch]

Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Picc Puncture Care Kit.Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth, 175-184[Custom Acrylic Stand Design Dry Erase Marker & Eraser]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Small Cleaning Brush ESD Anti Static Brush ESD Brush Black]

Whitaker,J.R.(1963),AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. Analytical Chemistry35(12), 1950-1953[New R&D Wire Cable Feeding Winding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production LineAuthor for correspondence