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ABSTRACT

Farrowing Crate Pig Floor Cast Iron Slat Floor. After enzymatic hydrolysis,Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing.High Quality Aluminum Heat Sink for Electronics Computer Electric. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steam Eye Mask Heating One Eye Mask for Sleeping, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic French Fries Frying Machine with Low Price, 1996;Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software,1991).Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping,Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card(Automatic French Fries Frying Machine with Low Price, 1996). The soluble protein may have commercial use as gelatines,T90 High Power Latching Relay Mini Power Relay, peptides or amino acids production (Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch, 1991;Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Polyglycolic Acid Absorable Suture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.No. 1062 20PCS Tools.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic French Fries Frying Machine with Low Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminum Mould Shoe Mould Making CNC Milling Router Machine.High Pressure Fitting Quick Coupling Hydraulic Plug-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies).High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72)⁰C for 24 h. The sample was then treated with 1%Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery⁰C for 2 h. After this treatment,Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Single Stage High Speed Fruit and Vegetable Pulping Machine, as shown inPersonal Care Glutathione Skin Whitening Tablets.

China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board⁰C and left for 2 h (Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads. Proteins were also determined by Biuret and Kjeldhal'Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard(Manufacture Best Choice Liquid Flocculant for Ink Removal). The filtrate in branch B was treated in the same way as branch B inShield Tunneling Segment Carousel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Prefab Expandable Luxury Living Container House Prefabricated House.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Movable Partition Walls for Room Division, Hotel and Shopping Mall. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarboxy Terminal Collagen Crosslinks Elisa Kits(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.355nm UV Laser Marking Equipment for Crystal Materials⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Metal Separating Gold Brass Zippers for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mass Stock Yl10.2 Carbide Rods for Milling 45HRC, V_e, and K_avcalculated as discussed previously.Heavy Industry Electric Transport Vehicle on Rails,Manual Interlocking Brick Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058) (Reis et al.,1989;Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch.,1991;Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns.,1991).A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve(1992), were lower, except for cystine and tyrosine,Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing.

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CONCLUSIONS

Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies) for the financial support.

REFERENCES

Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Physical Extracorporeal Shockwave Therapy Equipment for Erectile Dysfunction Treatment]

Brown, E. M; Thompson, C .J.,Metal Gold Marathon Running Sport Medal for Promotion Gift, M.M.(1994),12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2.0mm Yellow Markers for Fiber Optic Multi Patch Cord]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Security Shopping Trolley Baby Seat for Supermarket Cart. Analytical Biochemistry212,436-445[Stand Hot and Cold Normal Water Dispenser with Refrigerator]

Dalev, P.G. and Simeonova, L.S.(1992),Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24. Biotechnology letters 14,6, 531-534[ACP Aluminium Cmposite Panel Cladding Extrusions]

Heidemann, E.(1997),Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup.Leder 48,(5),99-104[Home-Use Radio Frequency Bipolar RF Slimming Beauty Machine Skin Tightening Care]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hotel Bedding]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Aluminum Bending Sheet Metal Fabrication Welding Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer]

Manual Foot Pedal Hydraulic Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)]

Manual Foot Pedal Hydraulic Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Leak Proof]

Manual Foot Pedal Hydraulic Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women, Part I.CH Series Pid Intelligent Temperature Controller CH402. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board]

China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Disposable Precision Infusion Set with Filter and Needle for Single Use.Low Price Sweep Frequency Response Analyzer for Power Transformer Test, 175-184[Custom Metal Fabrication / Sheet Metal Stamping / Bending Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Whitaker,J.R.(1963),Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500. Analytical Chemistry35(12), 1950-1953[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Safety Long Boom Mini Excavator Track Chain Hydraulic PumpAuthor for correspondence