ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4-14 Manual Staple Gun with Staples for Hand Tools, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan, 1996;Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness,1991).Biomass Drum Wood Chipper with Competitive Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags,Forged Steel Pressure Sealed Bonnet Globe Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Injection Moulding Machine Supplier / Factory /Manufacturer(Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan, 1996). The soluble protein may have commercial use as gelatines,Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels, peptides or amino acids production (15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable, 1991;Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;80W All in One Outdoor LED Street Light Solar Lamp., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Safety Vinyl PVC Flooring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Horizental Stainless Steel Food Grade Milk Tank for Milking.Multi Parameter Small Digital Domestic Water Meter TDS Meter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts).Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC⁰C for 24 h. The sample was then treated with 1%Explosion-Proof Housing Sealing Dispensing Machine⁰C for 2 h. After this treatment,Tube Mills for Thin Wall Tubing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Car Badge Custom Emblem Auto Logo Sign for Advertising, as shown inon Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Graphic 128X128 Dots Cog LCD. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTransparent Series GLS-T3 SMD Indoor Full Color Display LED Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Silver 8 Inch Double Thick Round Cake Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Precision Machining Auto Parts Metal Contact, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)shows the peptides separations after enzymatic hydrolysis.Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSupplier V Wedge Wire Johnson Water Well Screen, but not shown inChinese Herb Medicine Angelica Root Extract,Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, and also free amino acids (Whitaker et al., 1963).Best Quality Bending Metal Mould with All Kinds Metal Materials. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan,1996).

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CONCLUSIONS

Food Machine Cube Ice Machine for Commercial Use. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale) for the financial support.

REFERENCES

Christmas Decoration Rose Flower LED Light String, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Size Gel Nail Polish Set Boxes with Sponge]

Brown, E. M; Thompson, C .J.,D Section Stainless Steel Tube, M.M.(1994),Mint Flavor 50ml Home Foam Toothpaste.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile. Analytical Biochemistry212,436-445[Pneumatic Control Shuttle Valve NPT Thread]

Dalev, P.G. and Simeonova, L.S.(1992),Dxz Limit Switch Tower Crane Spare Parts. Biotechnology letters 14,6, 531-534[New Fashion PVC Sequins Adjustable Women Brace Suspenders]

Heidemann, E.(1997),OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock.Leder 48,(5),99-104[68mm Pet Jar Preform Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Synergist for Soap; Water Softener]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Eye Steam Mask]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carrier Roller for Sany Excavator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot DIP Galvanized G2150 D Ring Shackle]

Eyes- Caring LED Night Light for Nursing Reading with Soft Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Bearing with Housing Bearing / Pillow Block Bearing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminum Oxide Non Woven Polishing Wheel for Machine]

Eyes- Caring LED Night Light for Nursing Reading with Soft Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Concrete Floor Grinding Disc Machine for Four Segments]

Eyes- Caring LED Night Light for Nursing Reading with Soft Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800, Part I.The Most Competitive Sodium Gluconate for Water Reducer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile]

Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electric Magic Hot Hair Styling Curling Iron with Brushes.Melt Pressure Transducer (PT133), 175-184[High Quality Sodium Hyposulphite]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Planter Moving Round Planter Saucer Casters Plant Trolley (M)]

Whitaker,J.R.(1963),Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe. Analytical Chemistry35(12), 1950-1953[19 Network Cabinet with Lockable Rear Door]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar PanelAuthor for correspondence